Dual Labeled Probes for all type of qPCR instruments
Dual Labeled Probes are designed to increase the efficiency and specificity of quantitative PCR and allow multiplexing.
Product specifications:
- Available synthesis scales: 0.01 - 1.0 µmol
- Probe lengths: 5-40 bases (wobbles non-defined ratio possible)
- HPLC purified by default
- TAT: 3–5 working days (1.0 µmol scales takes longer)
- Delivery format: dried or liquid at selected concentration
- QC report incl. MALDI-TOF MS spectra free of charge
A ready-to-use qPCR probe dilution buffer (10 mM Tris-HCl; 1 mM EDTA; pH 8) is provided along with all probes (1 ml for up to 3 probes).
Available dye-quencher combination:
| 5' Reporter |
Abs [nm] |
Em [nm] |
3' Quencher |
Price-Cat. |
| FAM |
495 |
520 |
TAM, BHQ1, BBQ650, TQ2 |
1 |
| TET |
521 |
536 |
TAM, BHQ1 |
1 |
| JOE |
520 |
548 |
TAM, BHQ1, TQ2 |
1 |
| Yakima Yellow |
530 |
549 |
BHQ1, Eclip, TQ2 |
2 |
| HEX |
535 |
556 |
TAM, BHQ1, BHQ2, TQ2, TQ3 |
1 |
| Cyanine3 |
552 |
570 |
BHQ1, BHQ2 |
1 |
| ROX |
575 |
602 |
BHQ2, TQ3 |
1 |
| Texas Red |
583 |
603 |
BHQ2, TQ3 |
1 |
| ATTO 647N |
644 |
669 |
BHQ2, BBQ650, TQ3 |
2 |
| Cyanine5 |
649 |
670 |
BHQ2, BBQ650, TQ3 |
1 |
120Q
Principle of dual labeled probes
Dual labeled probes used in quantitative real-time PCR systems take advantage of the 5' -> 3' exonuclease activity of Taq polymerase.
Dual labeled probes contain a 5' fluorescent reporter and a 3' quencher which anneals between the PCR primers.
During the extension phase of PCR, the 5' -> 3' exonuclease activity of Taq polymerase cleaves the fluorescent reporter from the probe. The amount of free reporter accumulates as the number of PCR cycles increases. The fluorescent signal from the free reporter is measured in real time and allows the quantification of the target sequence.