Primers and probes recommended by the CDC & WHO
Eurofins Genomics can provide the primer and probe sequences recommended by the CDC and the WHO to researchers working on the coronavirus.
The sequences were publically provided by the CDC here and the WHO here. Researchers can order these primers and probes to create a panel in their lab, following the panel instructions from the CDC.
These materials are provided for research purposes only.
CDC Approved Sequences*
| Name |
Description |
Sequence (5' -> 3') |
Label |
Working Conc.
|
| 2019-nCoV_N1-F |
2019-nCoV_N1
Forward Primer
|
GAC CCC AAA ATC AGC GAA AT |
- |
20 µM |
| 2019-nCoV_N1-R |
2019-nCoV_N1
Reverse Primer
|
TCT GGT TAC TGC CAG TTG AAT CTG |
- |
20 µM |
| 2019-nCoV_N1-P |
2019-nCoV_N1
Probe
|
FAM-ACC CCG CAT TAC GTT TGG TGG ACC-BHQ1 |
FAM,
BHQ-1 |
5 µM |
| 2019-nCoV_N2-F |
2019-nCoV_N2
Forward Primer
|
TTA CAA ACA TTG GCC GCA AA |
- |
20 µM |
| 2019-nCoV_N2-R |
2019-nCoV_N2
Reverse Primer
|
GCG CGA CAT TCC GAA GAA |
- |
20 µM |
| 2019-nCoV_N2-P |
2019-nCoV_N2
Probe
|
FAM-ACA ATT TGC CCC CAG CGC TTC AG-BHQ1 |
FAM,
BHQ-1 |
5 µM |
| 2019-nCoV_N3-F |
2019-nCoV_N3
Forward Primer
|
GGG AGC CTT GAA TAC ACC AAA A |
- |
20 µM |
| 2019-nCoV_N3-R |
2019-nCoV_N3
Reverse Primer
|
TGT AGC ACG ATT GCA GCA TTG |
- |
20 µM |
| 2019-nCoV_N3-P |
2019-nCoV_N3 Probe
|
FAM-AYC ACA TTG GCA CCC GCA ATC CTG-BHQ1 |
FAM,
BHQ-1 |
5 µM |
| RP-F |
RNAse P
Forward Primer
|
AGA TTT GGA CCT GCG AGC G |
- |
20 µM |
| RP-R |
RNAse P
Reverse Primer
|
GAG CGG CTG TCT CCA CAA GT |
- |
20 µM |
RP-P
|
RNAse P
Probe
|
FAM – TTC TGA CCT GAA GGC TCT GCG CG – BHQ-1 |
FAM,
BHQ-1 |
5 µM |
>> Order Now
WHO Approved Sequences
|
Assay
|
Name |
Sequence (5' -> 3') |
Comment
|
|
RdRP
gene |
RdRP_SARSr-F2 |
GTGARATGGTCATGTGTGGCGG |
Use 600 nM per reaction |
|
| |
RdRP_SARSr-R1 |
CARATGTTAAASACACTATTAGCATA |
use 800 nM per reaction |
| |
RdRP_SARSr-P2 |
FAM-CAGGTGGAACCTCATCAGGAGATGC-BBQ |
Specific for 2019-nCoV,
will not detect SARS-CoVuse
100 nM per reaction
and mix with P1 |
| |
RdRP_SARSr-P1 |
FAM-CCAGGTGGWACRTCATCMGGTGATGC-BBQ |
Pan Sarbeco-Probe,
will detect 2019-nCoV, SARS-CoV
and bat-SARS-related CoVsuse
100 nM per reaction and mix with P2 |
| |
|
|
|
| E gene |
E_Sarbeco_F1 |
ACAGGTACGTTAATAGTTAATAGCGT |
Use 400 nM per reaction |
| |
E_Sarbeco_R2 |
ATATTGCAGCAGTACGCACACA |
Use 400 nM per reaction |
| |
E_Sarbeco_P1 |
FAM-ACACTAGCCATCCTTACTGCGCTTCG-BBQ |
Use 200 nM per reaction |
Ordering
We recommend to use the qPCR Assay order page to order the optimised and validated primer and probe sequences.
To order all recommended RdRP gene primers and probes, please use our Dual Labelled Probe and qPCR Primer order page.
Publications
- (2020) 2019 Novel Coronavirus (2019-nCoV), Wuhan, China. [Online] US Centers for Disease Control and Prevention. [Accessed 28 Jan 2020].
- (2020) 2019-Novel coronavirus (2019-nCoV) real-time rRT-PCR panel primers and probes. US Centers for Disease Control and Prevention. [Accessed 28 Jan 2020].
- (2020) Real-time RT-PCR panel for detection 2019-novel coronavirus. US Centers for Disease Control and Prevention. [Accessed 28 Jan 2020].
- (2020) Emergency use authorization. [Online] US Centers for Disease Control and Prevention. [Accessed 28 Jan 2020].
Note
In order to prevent any cross-contamination of primers and probes with control plasmids, our standard procedure is to spatially separated our production of oligos and genes/plasmisds by separate rooms and air systems!