NGS Favourites: Exome sequencing with IonTorrent
The NGS Favourites are predesigned for one application and contain all steps needed for a successful NGS project.
You can directly order this Favourite online in our new NGS ordering wizard. Here you are free to select the number of samples and additional services.
- Whole exome enrichment with Ion AmpliSeq Exome Kit
- Total design coverage is 58 Mbp including padding and flanking regions
- Clonal amplification by emulsion PCR
- Sequencing with IonProton using Ion PI v2 Chip (2 samples per chip)
- Automated variant analysis and driver mutation identification using the Ion Reporter software
- Data delivery on USB
- Delivery time is 5-10 working days per set of 8 samples
- Service available for sample numbers from 1-96
- Minimal input material is 250 ng high molecular weight DNA
The PCR based strategy exome sequencing approach enables a very simple, specific and fast workflow. Profit from exome sequencing with high uniformity and with >94% of targeted bases covered at >10-fold.
Which project is suitable for these NGS Favourites?
- Customers with projects of various sizes
- Projects with samples requiring fast turnaround times, such as clinical samples
- Projects with low input DNA samples