TubeSeq Service - Most Powerful DNA Sequencing

The TubeSeq Service is sequencing with the extra bit

Combined power of automation and expertise.

The Eurofins Genomics' TubeSeq Service combines long term expertise in automated Sanger sequencing processes with the handling of samples, which require bespoke solutions. 

Highlights of the TubeSeq Service include:

• Order and re-order up to 8 reactions per sample
• Sequencing reactions can be paid with TubeSeq Labels & TubeSeq Coupons
• All types of sample material, incl. large constructs, are possible
• Option for Power Read Upgrade* for complex sequences
• Standard DNA reads are available next day after sample receipt

The benefits of our TubeSeq Service at a glance:

• High quality read lengths of up to 1,100 bases
• Free Q20, Q30 or Q40 monitored single strand data available
• DNA preparation and PCR purification is available
• Concentration adjustment for DNA samples can be selected
• Advanced analysis options are available, for free
• Different sequencing result files can be selected per order
• Secure online archive of sequence data for 100 days
• Free one time repeat, in case of technical failure
• Storage of template DNA and ordered primers for 4 weeks
• Storage of ordered primers for one year, on request
• Easy re-order options from stored DNA samples

*In case your samples are >30kbp and/or your samples contain difficult-to-read stretches, select the Power Read Upgrade. We will perform special cycling conditions or chemistry to achieve best sequencing results. A free repetition is included. This service requires a longer processing time.

Sending samples the right way for our TubeSeq Service

Sending samples according to the requirements below helps us to do our job better and provides you with accurate results.

Sample submission

• Use 1.5 ml safe-lock tubes for your templates and primers
• Do not tape or wrap tubes with parafilm. Safe-lock tubes offer perfect sealing and evaporation protection
• Label your sample tube with our barcode labels. Either with the prepaid TubeSeq Labels or free Tube Labels
• Use our TubeSeq Coupons to pay for addtional reactions
• Label your enclosed primers with our free Tube Labels
• Use a water resistant marker for any additional labeling of your tubes

Sample preparation

Use the following concentration and volume for your samples:

Quantify the template concentration via agarose gel or a photometer to ensure accurate results.

Premixed samples (a mixture of template and primer):

• Templates should consist of 15 µl purified DNA with either of the concentrations given in the table above
• Add 2 µl of primer with a concentration of 10 pmol/µl (10 µM)
• Ensure that the total volume of your premixed sample is 17 µl

Sequencing primers

Optimum primer conditions:

• Primers must not contain phosphorylation or fluorescent dyes
• The optimum primer length is between 16-25 bases
• The primer melting temperature (Tm) should be 50 - 62°C
• The GC content of the primer should be 35-60%
• Ideally one G or C should be located at the 3' primer end
• The number of 3' Gs or Cs should not exceed 2 Gs or Cs
• If possible, avoid >3 identical bases in a row in the sequence
• Label your enclosed primers with our free Tube Labels

Primer concentration and volume:

• Exactly 10 pmol/µl primer concentration is required per sequencing reaction
• Each primer must have a total volume of 15 µl (double distilled water or 5mM Tris-HCl); 5 µl of primer volume is required for every additional sequencing reaction
• Concentration of primers with wobble bases must be calculated according to the following formula: n^X x Conc_Primer

n = number of bases within a wobble according to IUPC code, X = number of wobbles within the primer sequence. [e.g. 1 V (AGC) = 3¹ x 10 pmol/µl; 2 V (AGC) (AGC) = 3² x 10 pmol/µl]

DNA Sequencing has never been so easy

Just stick one TubeSeq Label on your sample tube and enter the barcode details to identify your sample. Conveniently use our TubeSeq Coupons to pay for addtional reactions.

You can also benefit from a variety of convenient online features to manage your TubeSeq Labels and TubeSeq Coupons:

Multiple options to define the rights of use

• TubeSeq Labels can be used by anyone with the same customer number
• The usage rights for TubeSeq Labels can be restricted to your account with one click in your sequencing preferences.
• You can also define a group of people who are allowed to use your TubeSeq Labels and your TubeSeq Coupons under "My Groups".
• TubeSeq Labels and TubeSeq Coupons can be transferred to any other account under "My Sanger Kits & Barcodes"

Automatic reminder to order again

• You always see the actual number of remaining prepaid reactions under "My Sanger Kits & Barcodes" where a traffic light signals when they are running low.
• In your sequencing preferences you can select to receive a reminder by email where you can specify the critical minimum amount of your TubeSeq Labels.

Overview of status and usage details

• Under the "Prepaid Tube Barcodes" tab on the "My Sanger Kits & Barcodes" page you can find full details of your purchased TubeSeq Labels and Coupons. 
• You can search, filter and sort to find specific barcodes.
• The details of users and sequencing orders are displayed in a tooltip by positioning your mouse cursor over the row of interest.