The unwanted
Not all genes are balanced and easy to work with. Some of them, most probably the important ones, can be really quirky and pretty difficult to handle.
Our complex gene service can handle those unwanted difficult genes. Independent on size, GC content or structure we will synthesise any gene for you.
Submit the sequence of interest in electronic format and we will synthesise your DNA fragment. Every synthetic gene is cloned into a standard vector by default or into a vector of choice, if requested. 100% accuracy of gene synthesis is guaranteed and verified by DNA sequencing.
Properties:
Eurofins Genomics offer complex gene synthesis services:
- for any size
- for high and low GC contents
- when long repeats are present
- when secondary structures are present
Use our expert consultation on any topic e.g. to find the optimal expression system, to check your sequence in advance, or to design your perfect gene in silico.
You also have the possibility to optimise your sequence using GENEius, our optimisation software.
Processing of your genes
The following steps describe our sophisticated gene synthesis process:
- If you would like your sequence to be checked prior synthesis
- To find the optimal expression system
- To design your perfect gene in silico
- To decide whether gene optimisation is necessary
Using our proprietary software GENEius, the supplied amino acid or DNA sequences are optimised and adapted, e.g. for codon usage to any organism. With GENEius prevents direct or inverted repeats and hairpins and facilitates the even distribution of G‘s and C‘s. Even the incorporation of "good motifs" is easily achievable with the GENEius software.
Try our GENEius light and discover what our optimisation can do for you.
When you have submitted your desired sequence we start with the experimental design. Therefore we divide the gene in overlapping oligonucleotides for gene synthesis by using GENEius.
After design, the oligonucleotide synthesis phase begins. Our oligonucleotide synthesis facility - with more than 25 years of experience - guarantees highest quality and fast turnaround times.
Our gene synthesis is closely linked to the oligonucleotide production. The lab is a perfect combination of robotics and human know how. Aside from automation, our project managers ensure best practise project layouts and stay in close touch with you if any questions arise.
Based on your individual needs, your gene can be subcloned into one of our standard vectors or into any other vector of choice.
The standard vector maps can be found here. We use "a" high copy cloning vector with either ampicillin or kanamycin resistance.
More details about different vector options can be found under scales & possibilities.
Quality that you can count on: our inhouse sequencing facility guarantees 100% accuracy of your synthetic gene by double-strand DNA sequencing.
For plasmid DNA preparation, a non manual mini-prep is generally performed free of charge as a standard procedure.
For higher yields, just order one of our manual plasmid preps yielding up to 10mg of your plasmid.
Endotoxin-free preparation for mega and giga scales are also available.Read more about our options for plasmid preparation in scales & possibilites.
We provide you with a quality assurance report and a delivery note after your synthetic gene has been synthesised via Ecom, our online ordering system.
The quality assurance report contains:
- Detailed project specifications
- Complete plasmid description
- Restriction map
- DNA sequence information
Strict confidentiality is ensured by every project. The high quality of our service is overseen by a professional quality management.
We are certified according to DIN EN ISO 9001.
Scales & possiblities
Plasmid preparation and subcloning adapted just for you.
With our gene synthesis service, we offer a wide range of plasmid preparation scales and different options for your cloning vector.
Subcloning
Choose from several options for your cloning vector:
- Eurofins Genomics standard vector - for free -
- Send us your personal vector
Your gene is normally cloned in a standard vector for free. This vector contains either ampicillin or kanamycin resistance.
Plasmid Preparation
- Mini prep scale: 1-10µg plasmid DNA - for free -
- Midi prep scale: 15-50µg plasmid DNA
- Maxi prep scale: 100-500µg plasmid DNA
- Mega prep scale: 500µg-2.5mg plasmid DNA
- Giga prep scale: up to 10 mg plasmid DNA
You receive a free DNA mini prep that is carried out automatically on our robots. If the amount of DNA is insufficient, you can choose one of the scales above.
If you order either the mega or giga prep's, an endotoxin-free preparation procedure is available. For maxi prep scale Animal-free production is available.
Additional services
Linearisation of your gene
Linearisation of your gene is offered in combination with Midi and Maxi prep scales.
Completion of the digest will be checked via Agarose gel analysis. Some undigested plasmid might still be present in the final reaction.
For linearised mini- and midiprep plasmids the heat-inactivated digest reaction will be shipped.
Extra purification via spun columns for Mini or Midi preps is possible on request.
If the enzyme cannot be heat-inactivated additional spun column purification is available on request.
For Maxiprep alcohol precipitation is available. Shipment of pellet or linearised plasmid in solution is possible.
The standard service is available with the following enzymes: EcoR1, Nhe1, Not1, SacI, SaII, ScaI, XbaI, XhoI. Other enzymes are possible, but Eurofins will have to check if those enzyme can be made available free of charge. In some cases where Eurofins has to buy the enzyme, the enzyme price will have to be charged additionally.