Custom DNA Oligos

Synthesis and postprocessing in ultra high throughput.

• Available for all unmodified DNA oligos
• Deprotected and free from salt
• Accurate quantification - Variability of max. +/- 20% 
• Fast TAT of 1-3 working days up to 60mer, 0.01 - 1.0 µmol scale
• TAT of > 61 mer or 10.0 µmol scale 4-10 working days

Make use of a NightXpress delivery by placing your SaltFree Oligo order before 9:00 am CET

Minimum and average yields (OD) per scale and length:

The synthesis scale indicates the initial amout of 3'-bases. Average yields are statistical values and vary due to oligo sequences with GC content >50%, >3 purine streches, or strong secondary structures. 

HPSF is a size exclusion method by gel filtration and stands for High Purity Salt Free. 

• Available for all unmodified and some modified oligos
• Cleaned from chemicals and truncated sequences
• Free from any disturbing salts
• Accurate quantification - Variability of max. +/- 10% 
• Purity of >70 % for 18-35mer oligos
• Fast TAT of 1-4 working days up to 60mer, 0.01 - 1.0 µmol scale
• TAT of > 61 mer or 10.0 µmol scale 6-10 working days

Minimum and average yields (OD) per scale and length for unmodified oligos:

Minimum yields (OD) per scale for modified oligos:

The synthesis scale indicates the initial amout of 3'-bases. Average yields are statistical values and vary due to oligo sequences with GC content >50%, >3 purine streches, or strong secondary structures.

HPLC means High Performance Liquid Chromatography. The purification method with high separation efficiency.

• Available for all type of DNA oligos
• Removal of truncated sequences
• Accurate quantification - Variability of max. +/- 10% 
• Purity of ≥80 % for up to 120 mers
• TAT: 5-7 working days up to 60mer, 0.01 - 1.0 µmol scale
• TAT of > 61 mer or 10.0 µmol scale 10 working days

Minimum and average yields (OD) per scale and length for unmodified oligos:

Minimum yields (OD) per scale for modified oligos:

The synthesis scale indicates the initial amout of 3'-bases. Average yields are statistical values and vary due to oligo sequences with GC content >50%, >3 purine streches, or strong secondary structures.

All relevant documents are provided in your online account:

• Oligo synthesis report
• Plate report
• Delivery note
• Quality report incl. QC spectra, if requested

To ensure a smooth ordering process, here are some hints how to order custom DNA oligos in plate format.

Ordering online:

• Up to 12 plates can be specified via file-upload or copy & paste
• One plate must contain a minimum of 12 oligos
• Modifications can be entered directly in the sequence
• Concentration and volume can be specified
• Plate copies as additional service can be selected
• A plate overview enables you to validate and edit single wells

Available plate types:

• 96well, 1.2 ml, round wells
• 96well, 0.8 ml, round wells
• 96well, 2.2 ml, deep well plates
• 96well, 0.2 ml PCR plates

Good to know:

Plate oligos are delivered in the selected plate type with the required volume (max. 500µl resp. 150µl for PCR Plates) for the selected concentration. 

For additional plate copies (aliquot-plates) or mixture plates the requested volume is taken from the mother plate.

The remaining oligos in the mother plate are always provided along with the plate copies or the mixture plates without additional charge.

Important for mixture plates:

• Define the plates for the forward and reverse primers (e.g. Plate1_ for, Plate1_ rev)
• Use the same primer names accompanied by the suffix “.for” or “.f” and “.rev” or ”.r”
• Select the concentration of the forward and reverse primers
• Specify the final concentration and volume of the primer mix in the order comment

Custom projects:

If you need additional plate formats, a special normalisation or pooling service, please send us your request!