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EVOcard

Oligonucleotide Synthesis

Gene Synthesis & Molecular Biology

Sanger Sequencing

Next Generation Sequencing

Genotyping & Gene Expression

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Custom DNA Oligos for all kinds of application

 

 

Customised DNA oligonucleotides according to your specifications

 


Select from the huge variety of modifications, purification options and synthesis scales.

 

 

 

 

 

 

Custom DNA Oligos in Tubes

 

  • Huge variety of DNA modifications available
  • Synthesis scales from 0.01 µmol to 10 µmol
  • Purification options: Salt Free, HPSF, HPLC
  • Sequence lengths:
    • 5 - 120 bases for unmodified oligos
    • 5 - 80 bases for modified oligos (5-50 bases for some 3' modifications)
  • Quality control by OD measurement and MALDI-TOF MS
  • Delivery in 2ml screw cap tubes
  • Delivery format: lyophilised or concentration adjusted
  • Aliquoting service available

 

Custom DNA Oligos in Plates

 

  • Large variety of DNA modifications available
  • Synthesis scales: 0.01 µmol, 0.05 µmol and 0.2 µmol
  • Purification options: Salt Free, HPSF, HPLC
  • Sequence lengths:
    • 5 - 120 bases for unmodified oligos
    • 5 - 80 bases for modified oligos (5-50 bases for some 3' modifications)
  • Quality control by OD measurement and MALDI-TOF MS
  • Delivery format: Liquid at selected concentration
  • Mixing of forward and reverse primers possible
  • Generation of plate copies (aliquot - plates) available

 

 

 

 

 

 

 Related Information

 

Salt Free Oligos

Synthesis and postprocessing in ultra high throughput.

  • Available for all unmodified DNA oligos
  • Deprotected and free from salt
  • Accurate quantification - Variability of max. +/- 20% 
  • Fast TAT of 1-3 working days up to 60mer, 0.01 - 1.0 µmol scale
  • TAT of > 61 mer or 10.0 µmol scale 4-10 working days


Minimum and average yields (OD) per scale and length:

Length [bases] /
Synthesis scale
5 - 17
18 - 35
36 - 50
51 - 80
81 - 120
0.01 µmol
minimum  3.0 4.0 7.0 xxx xxx
average 6.0 8.5 14.5 xxx xxx
0.05 µmol minimum   4.0 6.0  10.0  10.0  10.0 
average  8.0 12.5  20.0  25.0  30.0 
0.20 µmol minimum  10.0 12.0 20.0 25.0 25.0
average 20.0 25.0  40.0  35.0  na 
  1.0 µmol minimum  30.0 35.0  50.0  60.0  60.0 
average na na na  na  na 
   10 µmol minimum   300 500 400  400  200 
average na na na na na

The synthesis scale indicates the initial amout of 3'-bases. Average yields are statistical values and vary due to oligo sequences with GC content >50%, >3 purine streches, or strong secondary structures. 

 

HPSF Purified Oligos

HPSF is a size exclusion method by gel filtration and stands for High Purity Salt Free. 

  • Available for all unmodified and some modified oligos
  • Cleaned from chemicals and truncated sequences
  • Free from any disturbing salts
  • Accurate quantification - Variability of max. +/- 10% 
  • Purity of >70 % for 18-35mer oligos
  • Fast TAT of 1-4 working days up to 60mer, 0.01 - 1.0 µmol scale
  • TAT of > 61 mer or 10.0 µmol scale 6-10 working days


Minimum and average yields (OD) per scale and length for unmodified oligos:

Length [bases] /
Synthesis scale
5 - 17
18 - 35
36 - 50
51 - 80
81 - 120
0.01 µmol
minimum  1.5 2.0 2.5 xxx xxx
average 3.0 5.0 9.0 xxx xxx
0.05 µmol minimum  2.0 3.0  5.0  3.0  3.0 
average  4.0 7.5  15.0  15.0  15.0 
0.20 µmol minimum  4.0 6.0 10.0 10.0 10.0
average na 15.0  25.0  25.0  22.0 
  1.0 µmol minimum  15.0 25.0  30.0  30.0  30.0 
average na 40.0 50.0  45.0  na 
   10 µmol minimum   150 300 200  200  150 
average na na na na na


Minimum yields (OD) per scale for modified oligos:

Synthesis scale
0.01 µmol
0.05 µmol
0.2 µmol
1.0 µmol
10 µmol
HPSF
minimum OD 2.0 3.0 5.0 10.0 100

 

The synthesis scale indicates the initial amout of 3'-bases. Average yields are statistical values and vary due to oligo sequences with GC content >50%, >3 purine streches, or strong secondary structures.

 

HPLC Purified Oligos

HPLC means High Performance Liquid Chromatography. The purification method with high separation efficiency.

  • Available for all type of DNA oligos
  • Removal of truncated sequences
  • Accurate quantification - Variability of max. +/- 10% 
  • Purity of ≥80 % for up to 120 mers
  • TAT: 5-7 working days up to 60mer, 0.01 - 1.0 µmol scale
  • TAT of > 61 mer or 10.0 µmol scale 10 working days


Minimum and average yields (OD) per scale and length for unmodified oligos:

Length [bases] /
Synthesis scale
5 - 17
18 - 35
36 - 50
51 - 80
81 - 120
0.01 µmol
minimum  1.0 1.5 2.0 xxx xxx
average 2.5 4.0 6.0 xxx xxx
0.05 µmol minimum  2.0 2.5  3.0  3.0  3.0 
average 3.5 6.0  10.0  9.5  9.3 
0.20 µmol minimum  4.0 6.0 8.0 8.0 8.0
average 6.5 13.5  17.5  14.0  10.0 
  1.0 µmol minimum  10.0 10.0  20.0  15.0  10.0 
average 18.5 35.0 40.0  25.0  na 
   10 µmol minimum   80 150 180  120 80
average na na na na na


Minimum yields (OD) per scale for modified oligos:

Synthesis scale
0.01 µmol
0.05 µmol
0.2 µmol
1.0 µmol
10 µmol
HPLC
minimum OD 1.0 2.0 3.0 6.0 60

 

The synthesis scale indicates the initial amout of 3'-bases. Average yields are statistical values and vary due to oligo sequences with GC content >50%, >3 purine streches, or strong secondary structures.

 

Documentation

All relevant documents are provided in your online account:

  • Oligo synthesis report
  • Plate report
  • Delivery note
  • Quality report incl. QC spectra, if requested

 

 

Ordering Oligos in Plates


To ensure a smooth ordering process, here are some hints how to order custom DNA oligos in plate format.


Ordering online:

  • Up to 12 plates can be specified via file-upload or copy & paste
  • One plate must contain a minimum of 12 oligos
  • Modifications can be entered directly in the sequence
  • Concentration and volume can be specified
  • Plate copies as additional service can be selected
  • A plate overview enables you to validate and edit single wells


Available plate types:

  • 96well, 1.2 ml, round wells
  • 96well, 0.8 ml, round wells
  • 96well, 2.2 ml, deep well plates
  • 96well, 0.2 ml PCR plates


Good to know:

Plate oligos are delivered in the selected plate type with the required volume (max. 500µl resp. 150µl for PCR Plates) for the selected concentration. 

For additional plate copies (aliquot-plates) or mixture plates the requested volume is taken from the mother plate.

The remaining oligos in the mother plate are always provided along with the plate copies or the mixture plates without additional charge.


Important for mixture plates:

  • Define the plates for the forward and reverse primers (e.g. Plate1_ for, Plate1_ rev)
  • Use the same primer names accompanied by the suffix “.for” or “.f” and “.rev” or ”.r”
  • Select the concentration of the forward and reverse primers
  • Specify the final concentration and volume of the primer mix in the order comment

 

Custom projects:

If you need additional plate formats, a special normalisation or pooling service, please send us your request!

 

 

 

 

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Quality is important for us

Our products and services are produced and performed under strict quality management and quality assurance systems.

Find certificates here