Molecular biology service excellence: Custom solutions
A box of chocolates contains a wide assortment of pralines in fascinating flavours and intriguing textures. Similar to a box of chocolate, we offer a wide range of molecular biology services to choose from.
All techniques listed below can be applied to many different tasks in molecular biology research. Our experts can help you define the perfect experimental set-up for your specific requirements anytime.
Molecular biological services
- Plasmid preparation services
From small scale prep's up to 20 mg of pure DNA
Mutation of your gene of interest for any downstream application
Everything from PCR products to full length cDNA and a whole lot more
Sequencing of genomic bacterial or fungal DNA up to 108 bp using a linker ligation technique
- DNA Barcoding
Bacterial, fungal, animal, plant or insect identification using amplification of the 16S rRNA, ITS, Cytochrome b, COI, etc. regions
- Oligo design project services
Design of specific PCR and RT-PCR primers, specific qPCR probes or molecular beacons and design of 50mer oligonucleotides for microarray experiments
Sequencing unknown genome regions
Identify unknown sequences in the vicinity of known sequences using LILIS-PCR.
Using minimal amounts of genomic DNA, we systematically sequence large nucleotide segments employing a PCR based technique: LILIS-PCR. This technique - established by our scientists - is used to circumvent a shotgun sequencing approach, and the necessity to sequence many recombinant clones.
Main applications for LILIS-PCR:
- Determining the sequence of a cosmid inserts
- Determining the sequence of other integration vectors
- Identify the integration site of transposons
Principle of LILIS-PCR