So many options for such a small sequencing primer
Benefit from the greatest flexibility to handle your sequencing primers.
Thanks to our DNA synthesis lab, bioinformatic know how and internal IT solutions you can choose how to provide us with your sequencing primers:
- Select from standard primers on stock
Select and manage your favourite standard primers from more than 80 different validated standard primers
- Design your specific sequencing primers
Our sequencing primer design tool lets you design your primers and easily order the primer synthesis
- Order specific sequencing primers
Specific primers can be ordered directly with your sequencing order. These primers are stored for 4 weeks and can be re-used during this time
- Enclose your own sequencing primers
You can send us your sequencing primers in separate tubes with your sample shipment by using Tube Barcode Labels
- Premix primer with DNA template
Sequencing primers can be added directly to your DNA template and sent to us as premixed sample with the TubeSeq Service, Mix2Seq, LightRun Tube, LightRun Plate or PlateSeq Service.
Please consider optimal primer conditions, concentration and volume as described in our sample submission guides.
If you prefer to store your sequencing primers, just order your SeqPrimer. The primers are shipped the very next day to you.
Standard primers - predefined, validated and free of charge
80 different predefined and validated standard primers are available on stock free of charge
- Primer listed in alphabetical order
- Full sequence information
- Validated quality by DNA sequencing
- Regularly updated and extended
||CCC TCA TAG TTA GCG TAA CG
||GCA AAT GGC ATT CTG ACA TCC
||GAC TGG TTC CAA TTG ACA AGC
||TAC TAT TGC CAG CAT TGC TGC
||CGC AAA TGG GCG GTA GGC GTG
||GGC AGC AGC CAC AGG TAA GA
||TTG CTG TTG CAC AGT GAT TC
||GGG ATG TTT AAT ACC ACT AC
The full lists of standard primers can be downloaded as pdf
Order the synthesis of a standard primer
The secret for great results
Precise sequencing primer design is essential for successful sequencing reactions.
Our sequencing primer design tool lets you define specific primer sequences. Optimum design criteria are considered to ensure improved specificity:
- Physical properties of the sequencing primers
Optimum primer lengths, G/C as 3’ clamp, GC-content, primer melting temperature (Tm), repeats and optimal primer-primer annealing scores
- Physical properties of the template
Best product length and GC-content range, primer Tm, repeats and optimal primer-template annealing scores
- Appropriate annealing temperature (Ta)
Avoid primers with secondary structures and self-dimer formation, primer dimer formation and any non-specific binding sites on the template
Just paste your DNA sequence in FASTA format and specify the region(s) for your sequencing primers and the number of sequencing primers required.
You get your optimum sequencing primers with just one click on the "design primer" button.
The "order now" button lets you order the synthesis of your designed primers.