Design, Order & Manage Your Sequencing Primers

So many options for such a small sequencing primer

Benefit from the greatest flexibility to handle your sequencing primers.

Thanks to our DNA synthesis lab, bioinformatic know how and internal IT solutions you can choose how to provide us with your sequencing primers:

Select from 80 standard primers on stock
Select and manage your favourite standard primers from more than 80 different validated standard primers.

Design your specific sequencing primers
Our sequencing primer design tool lets you design your primers and easily order the primer synthesis.

Order the synthesis of your sequencing primers
The synthesis of sequencing primers can be ordered directly with your sequencing order. These primers are stored for 4 weeks free of charge. Storage of synthesised primers for one year is available at additional cost. These stored primers can be re-used during this storage time. 

Enclose your own sequencing primers
You can send us your sequencing primers in separate tubes with your sample shipment by using our Primer Barcodes. 

Premix primer with DNA template
Sequencing primers can be added directly to your DNA template and sent to us as premixed sample with the TubeSeq Service, Mix2Seq, LightRun Tube, LightRun Plate or PlateSeq Service.

Please consider optimal primer conditions, concentration and volume as described in our sample submission guides.

If you prefer to store your sequencing primers in your lab, just order our SeqPrimer. 

Primer synthesis for your sequencing order for only 3.80 €*

Getting a primer synthesised along with your sequencing order has never been so easy, convenient and cheap!

There is no reason to order your sequencing primer separately or somewhere else:

Reliable quality you can trust
Every sequencing primer has the optimised and validated quality of our SeqPrimer to ensure:

• Accurate base detection and sequence interpretation
• Zero background sequence
• Sequences with highest possible trace score

Convenient and easy to order
Just enter the name and sequence of your primer to be synthesised on the sequencing order page.

• The sequencing primer is delivered to the Sanger sequencing lab before your samples arrive.
• They are stored in the sequencing lab for 4 weeks free of charge. 1 year storage on request at additional cost.
• During that time you can easily reuse them for further sequencing reactions.

Best price on the market
You will not find any comparable service and speed for this attractive price of 3.80 €*, only!

*Other currencies apply

The secret for great results

Precise sequencing primer design is essential for successful sequencing reactions.

Our sequencing primer design tool lets you define specific primer sequences. Optimum design criteria are considered to ensure improved specificity:

• Physical properties of the sequencing primers
  Optimum primer lengths, G/C as 3’ clamp, GC-content, primer melting temperature (Tm), repeats and optimal primer-primer annealing scores
• Physical properties of the template
  Best product length and GC-content range, primer Tm, repeats and optimal primer-template annealing scores
• Appropriate annealing temperature (Ta)
  Avoid primers with secondary structures and self-dimer formation, primer dimer formation and any non-specific binding sites on the template

Just paste your DNA sequence in FASTA format and specify the region(s)  for your sequencing primers and the number of sequencing primers required. 

You get your optimum sequencing primers with just one click on the "design primer" button.

The "order now" button lets you order the synthesis of your designed primers. 

Standard primers - predefined, validated and free of charge

80 different predefined and validated standard primers are available on stock free of charge

• Primer listed in alphabetical order
• Full sequence information
• Validated quality by DNA sequencing
• Regularly updated and extended

The full lists of standard primers can be downloaded as pdf

Order the synthesis of a standard primer