INVIEW Oncopanel All-in-one
Validated cancer panel for analysis of 591 key cancer-specific genes from tissue biopsy samples and genomic DNA
INVIEW Oncopanel All-in-one is the most comprehensive gene panel available for optimised detection of tumour-specific genomic alterations. The oncology panel is optimised for formalin-fixed paraffin embedded (FFPE) or fresh-frozen tissue samples, as well as genomic DNA samples, to deliver accurate genomic information on the status of clinically relevant driver mutations. This solid tumour panel uniformly and reproducibly covers the entire exons of about 600 characterised cancer genes, including tumour activators, tumour suppressors and biomarkers for drug resistance.

Typical applications for INVIEW Oncopanel All-in-one include:
- Comparative analysis of data from tissue biopsy and liquid biopsy (INVIEW Liquid Biopsy Oncoprofiling)
- Cancer screening by detection of well-known tumour-associated mutations such as driver mutations
- Determination of most important mutations for further studies
- Estimation of immune checkpoint inhibitor immunotherapy response via tumor mutational burden (TMB) biomarker analysis with tumor-only samples (paired matched healthy/normal control sample not necessarily required)
INVIEW Oncopanel All-in-one is suitable for clinical applications like:
- Molecular stratification
- Detection of resistance genes
- Guidance for treatment decisions
- Assignment of patients to clinical trials
- Post-treatment follow-up, especially for monitoring of multiple mutations
Highlights INVIEW Oncopanel All-in-one
- Highly uniform coverage of all exons in 591 cancer-associated genes
- Detection of relevant single nucleotide variations (SNVs) and insertions and deletions (InDels), structural rearrangements and copy number variations (CNV) in genes, selected promotor regions, miRNAs, and extra-exonic variants (see “Product Details”)
- Determination of tumor mutational burden (TMB) status of the patient
- Ultra-sensitive detection levels down to 1% allele frequency#
- Proprietary protocols for low-input DNA library preparation from challenging tissue and FFPE samples
Full service package offered by INVIEW Oncopanel All-in-one including
- Validated next-generation sequencing (NGS) cancer gene panel for analysis of key tumour-associated mutations in 591 protein-coding genes, selected promoter regions, miRNAs, and 285 extra-exonic variants (introns, regulatory regions, untranslated regions (UTRs) and intergenic regions)
- Reproducible detection of single nucleotide polymorphisms (SNPs), insertions and deletions (InDels)
- Determination of tumor mutational burden (TMB) based on all coding synonymous and non-synonymous eligible somatic mutations with in silico filtering of known and predicted germline variants
- Accurate detection of gene fusions between all covered genes, as well as 22 additional cancer-associated gene fusions
- Highly efficient target enrichment with latest Agilent SureSelect technology
- Target enrichment approach outcompetes common PCR-based enrichment with very uniform coverage and all exons of the genes covered
- Industry-leading sequence coverage and uniformity
- Detection of variants with sensitivity levels down to 1%#
- Optimised protocols for low-input DNA library preparation from FFPE or fresh-frozen tissue samples
- Professional bioinformatical analysis with optional use of QIAGEN’s QCI Interpret Translational software
- Analysis of copy number variations (CNVs) possible*
*CNV analysis should be performed in paired analysis of tumour versus matched normal tissue which is used as reference. In the case of tumour FFPE samples, we strongly recommend sending a healthy tissue FFPE sample from the same patient. Alternatively, tumour FFPE samples from at least seven other patients can be sent.
#Depending on the input DNA quality and genomic region LOD might be >1%
For fresh frozen tissue, we strongly advise that blood or healthy tissue from the same patient is sent. Alternatively, fresh frozen tissue from at least seven other patients or controls is recommended.
Product details
Accepted starting material for INVIEW Oncopanel All-in-one
- DNA
- FFPE tissue
- Fresh-frozen tissue
Specifications
- Hybridisation-based target capture using GATC optimised protocols and latest Agilent SureSelect technology
- Next-generation sequencing on Illumina platform
- FastQ files (sequences and quality scores)
- Alignment file (bam)
- SNP and InDel tables including annotated variants and effects (vcf, tsv)
- Tabels of copy gain and copy loss events (tsv)
- Table of gene fusion events (tsv)
- Comprehensive data analysis report (pdf)