Maps of regularly used Standard Vectors
Please find below the details and vector maps of our standard vectors that most often are used for gene synthesis.
Please note that we may change the pEX standard vectors to other cloning vectors, if necessary in the assembly process.
These alternative vectors can be e.g. pUC or pBluescript derivatives carrying the specified antibiotic selection marker Amp or Kan.
The restriction sites in the multiple cloning site and elsewhere in the backbone may be different in these alternative cloning vectors.
If you need details about our former Standard vectors (pEX-A, pEX-K, pEX-K2) or any other need additional information, just contact us!
Vector of your choice
Subcloning in only 4 days!
For express genes it is possible to order express subloning in a vector of your choice.
Specifications
- Only in combination with an express gene
- Turnaround time: 4 additional working days
(genes < 1000 bp; in total 8 days)
(genes > 1000 bp in total 10/11/15/17 days)
- A selection of various vectors is available in our online shop, Ecom
Please note:
- Only unmodified commercially available vectors up to 7kb with Ampicillin or Kanamycin selection marker can be used.
- Please provide at least 3 ug with ratio (260/280) 1.7 to 1.9 in solution (conc. ~200ng/ul).
- The vector should be labeled with name of the plasmid, volume and concentration.
- Please email the complete sequence of the vector as text file to following email address: genes-eu[at]eurofins.com.
- Vector DNA and sequence must be available at the latest two days after the order date. If possible please provide the tracking number for the shipment.
- Please note that subcloning can only be performed via two different sticky end restriction sites.
- Restriction sites must be located at least 6 bp apart from each other.
- Please use a padded envelope, clearly state the order number, and send your vector to:
Eurofins Genomics
GENE SYNTHESIS Lab
Anzinger Strasse 7a
85560 Ebersberg
Germany
The Express Subcloning service (available only in combination with Express Genes) can be performed with commercially available, unmodified vectors that must be provided by the customer.
Below you will find a list of vectors that are compatible with Express Subcloning (you can search for your vector using CTRL+F).
| Vectors suitable for Express Subcloning |
|
pBluescript II KS(–)
|
psiCHECK-1 |
pET-41a(+) |
| pBluescript II KS(+) |
psiCHECK-2 |
pET-42a(+) |
| pBluescript II SK(–) |
pUC18 |
pET-42b(+) |
| pBluescript II SK(+) |
pUC19 |
pET-42c(+) |
| pcDNA3.1 /CAT |
pUC57 |
pET-43.1a(+) |
| pcDNA3.1(-) |
pET-11a |
pET-43.1b(+) |
| pcDNA3.1(-)/myc-His /LacZ |
pET-11b |
pET-43.1c(+) |
| pcDNA3.1(-)/myc-His A |
pET-11c |
pET-44a(+) |
| pcDNA3.1(-)/myc-His B |
pET-11d |
pET-44b(+) |
| pcDNA3.1(-)/myc-His C |
pET-14b |
pET-44c(+) |
| pcDNA3.1(+) |
pET-15b |
pET-45b(+) |
| pcDNA3.1(+)/myc-His /LacZ |
pET-16b |
pET-47b(+) |
| pcDNA3.1(+)/myc-His A |
pET-17b |
pET-48b(+) |
| pcDNA3.1(+)/myc-His B |
pET-19b |
pET-49b(+) |
| pcDNA3.1(+)/myc-His C |
pET-20b(+) |
pET-50b(+) |
| pcDNA3.1/His /LacZ |
pET-21(+) |
pET-51b(+) |
| pcDNA3.1/His A |
pET-21a(+) |
pET-52b(+) |
| pcDNA3.1/His B |
pET-21b(+) |
pET-9a |
| pcDNA3.1/His C |
pET-21c(+) |
pET-9b |
| pcDNA3.1/Hygro /LacZ |
pET-21d(+) |
pET-9c |
| pcDNA3.1/Hygro(-) |
pET-22b(+) |
pET-9d |
| pcDNA3.1/Hygro(+) |
pET-23(+) |
pGEX-2T |
| pcDNA3.1/V5-His /LacZ |
pET-23a(+) |
pGEX-2TK |
| pcDNA3.1/V5-His A |
pET-23b(+) |
pGEX-3X |
| pcDNA3.1/V5-His B |
pET-23c(+) |
pGEX-4T-1 |
| pcDNA3.1/V5-His C |
pET-23d(+) |
pGEX-2T |
| pcDNA3.1/Zeo /CAT |
pET-24(+) |
pGEX-2TK |
| pcDNA3.1/Zeo(-) |
pET-24a(+) |
pGEX-3X |
| pcDNA3.1/Zeo(+) |
pET-24b(+) |
pGEX-4T-1 |
| pcDNA4/His /LacZ |
pET-24c(+) |
pGEX-4T-2 |
| pcDNA4/His A |
pET-24d(+) |
pGEX-4T-3 |
| pcDNA4/His B |
pET-25b(+) |
pGEX-5X-1 |
| pcDNA4/His C |
pET-26b(+) |
pGEX-5X-2 |
| pcDNA4/myc-His /LacZ |
pET-27b(+) |
pGEX-5X-3 |
| pcDNA4/myc-His A |
pET-28a(+) |
pGEX-6P-1 |
| pcDNA4/myc-His B |
pET-28b(+) |
pGEX-6P-2 |
| pcDNA4/myc-His C |
pET-28c(+) |
pGEX-6P-3 |
| pcDNA4/TO |
pET-29a(+) |
pGL3-Basic |
| pcDNA4/TO /LacZ |
pET-29b(+) |
pGL3-Control |
| pcDNA4/V5-His /LacZ |
pET-29c(+) |
pGL3-Enhancer |
| pcDNA4/V5-His A |
pET-30a(+) |
pGL3-Promoter |
| pcDNA4/V5-His B |
pET-30a(+) |
pPICZ A |
| pcDNA4/V5-His C |
pET-30b(+) |
pPICZ B |
| pcDNA5/FRT |
pET-30c(+) |
pPICZ C |
| pcDNA5/FRT/CAT |
pET-31b(+)
|
pPICZalpha A |
| pcDNA5/FRT/TO |
pET-32a(+) |
pPICZalpha B |
| pcDNA5/FRT/TO/CAT |
pET-32b(+) |
pPICZalpha C |
| pcDNA5/TO |
pET-32c(+) |
pESC-HIS |
| pcDNA5/TO /LacZ |
pET-33b(+) |
pESC-LEU |
| pcDNA6/myc-His /LacZ |
pET-39b(+) |
pESC-TRP |
| pcDNA6/myc-His A |
pET-3a |
pESC-URA |
| pcDNA6/myc-His B |
pET-3b |
|
| pcDNA6/myc-His C |
pET-3c |
|
| pcDNA6/V5-His /LacZ |
pET-3d |
|
| pcDNA6/V5-His A |
pET-40b(+) |
|
| pcDNA6/V5-His B |
pET-41a(+) |
|
| pcDNA6/V5-His C |
pET-41b(+) |
|
Please note:
The order must be placed before 3 p.m. CET. TAT period begins on the following day.
4-/6-/7-/11-/13-days means business days (Monday - Friday, excluding all public holidays at our facility in Ebersberg, Germany).
An additional express fee will be charged. If, against all expectations, we do not ship your gene on day 4/6/7/11/13, then no express fee will be applied.