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Oligonucleotide Synthesis

Gene Synthesis & Molecular Biology

Sanger Sequencing

Next Generation Sequencing

Genotyping & Gene Expression

 

 

Mix2Seq is the smartest
Sanger sequencing solution for premixed samples in tubes

 

 

SMART • EASY • QUICK

 


Experience ultimate innovation and highest convenience.

 

 

 

 

 

 

Highlights of the Mix2Seq Service:


Ultimate innovation

  • Buy one Mix2Seq Kit to pay for 96 reactions 
  • Share your Mix2Seq data with others via your group
  • Transfer Mix2Seq Kits with all rights to someone else

 

Highest convenience

 



Excellent quality & service

  • Get your data either overnight or until next day
  • Obtain reading lengths of up to 1100 high quality bases
  • Sequencing data are provided in the following file formats by default and are available online for 6 months:
    • .ab1 files - ABI trace files with all available raw data
    • .scf files -Trace files with base calls and quality values
    • .seq files - Clipped & unclipped FASTA sequences
    • .phd.1 files - Contains base call and quality information
    • .pdf quality report - Trace file with quality scores
    • FASTA file with all sequences
  • Under My Kits & Labels you can track your Mix2Seq Kit

 

 

 

 

 

 

 Related Information

 

Sample Preparation & Submission


4 little steps to optimum results

 

smartseq_step1 Your template should consist of 5 µl purified DNA in either of the following concentrations:
Plasmid DNA
up to 15kbp: 50-100 ng/µl
PCR products
: 150-300 bp: 1 ng/µl; 300-1000 bp: 5 ng/ µl; 1000 - 3000 bp 10 ng/µl
smartseq_step2 Add µl of your SeqPrimer with a concentration of 5 pmol/µl (5 µM).
smartseq_step3 Ensure that the total volume of your DNA / primer mix is at least 10 µl.
smartseq_step4 Pipet your premixed sample in one of the Mix2Seq tubes and seal the tube with the blue or yellow lid from the enclosed cap pad.

Do not forget to note the barcode number on the side of the tubes before you send us your samples!


Conditions

DNA templates must be purified. Sequencing reactions cannot be repeated and all reactions will be charged. Samples are not stored. For samples containing difficult-to-read streches (GC-rich, hairpin structurs...) we recommend our TubeSeq Service.

 

Sequencing Primers

 


Optimum primer conditions:

  • Primers must not contain phosphorylation or fluorescent dyes
  • The optimum primer length is between 16-25 bases
  • The primer melting temperature (Tm) should be 50 - 62°C
  • The GC content of the primer should be 35-60%
  • Ideally one G or C should be located at the 3' primer end
  • The number of 3' Gs or Cs should not exceed 2 Gs or Cs
  • If possible, avoid >3 identical bases in a row in the sequence
  • Label your enclosed primers with our free Tube Labels

 

Storage Times


Sample storage:

Premixed samples are not getting stored.


Data storage:

Sequencing data are provided in the following file formats by default and are available online for 6 months:

  • .ab1 files - ABI trace files with all available raw data
  • .scf files -Trace files with base calls and quality values
  • .seq files - Clipped & unclipped FASTA sequences
  • .phd.1 files - Contains base call and quality information
  • .pdf quality report - Trace file with quality scores
  • FASTA file with all sequences

 

 

 

 

Literature

 

 

 

 

 

 

 

 

Solutions you may also like

 

 

 

SeqPrimer


The perfect primer for
Sanger sequencing


>> READ MORE

 

 

 

PlateSeq Service


Sanger sequencing of all sample types in 96well plates


>> READ MORE

 

 

 

SeqPrimer NXP


The perfect sequencing primer next day at your bench


>> READ MORE

 

 

 

TubeSeq Service


Sanger sequencing in tubes with the extra bit


>> READ MORE

 

 

Quality is important for us

Our products and services are produced and performed under strict quality management and quality assurance systems.

Find certificates here