The adaptor ligation approach can be applied for short amplicons for Illumina sequencing.
With Illumina amplicon sequencing, extremely high coverage suitable for discovery of somatic mutations in complex DNA samples can be achieved. Proprietary protocols and years of NGS expertise help provide high quality data with extraordinary fast delivery times.
|Illumina adapter ligation library
100 - 200 ng PCR product / amplicons (up to 100 µl, concentration > 1 ng/µl
Amplicon size range should ideally range from 150 bp to 270 bp for sequencing with 5M reads pairs 2 x 150 bp
- Guaranteed 5M read pairs (10 million reads) per package, 2 x 150 bp
- Merging of overlapping paired-end reads
- Data sorting according to tags designed by Eurofins Genomics
- Data sorting according to customer supplied tag
Starting from less than two weeks, varies depending on individual project aims