Custom RNA Oligos

Single Strand RNA, O-Methyl-RNA / Chimerics & RNA qPCR Probes

RNA oligonucleotides ready for use in any application.

Introduction of our Custom RNA Portfolio:

Are you working on CRISPR applications, qPCR, RNA structure studies, or synthetic biology research?

With decades of expertise in oligonucleotide synthesis, Eurofins Genomics offers a comprehensive portfolio of high-quality RNA oligos and a broad selection of modifications:

Custom single strand RNA oligos
Custom single-stranded RNA oligos, 5 to 80 nucleotides long, are available desalted or HPLC-purified with various modifications to ensure reliable performance for your research.
O-Methyl-RNA Oligos & Chimerics
In chimeric antisense oligos, the incorporation of 2'-O-Methyl-RNA enhances resistance to nuclease degradation. These oligos combine DNA and RNA bases, often including modifications such as 2'-O-Methyl groups or PTOs.
RNA qPCR Probes
RNA qPCR probes are specifically optimized for real-time quantitative PCR, manufactured using advanced synthesis and purification techniques to ensure minimal background noise.

Discover the Benefits of our Custom RNA Oligo Portfolio:

Effortless online ordering through intuitive product pages
Flexible synthesis scales to perfectly match your project needs
Choose from desalted or HPLC-purified unmodified RNA oligos
All modified RNA oligos, qPCR probes, and 2’-O-Methyl-RNA / chimeric oligos come HPLC purified by default
Rigorous quality control with OD measurement and MALDI-TOF MS ensures outstanding reliability

Comprehensive documentation delivered with every order:

Detailed oligo synthesis report
Clear and precise delivery note

Exclusive free online tools to streamline your workflow:

Selectable label layouts for your oligo tubes
Complementary sequence calculator with direct ordering
Online status and delivery tracking to keep you informed every step of the way

>> FAQ's Oligonucleotides

>> Custom DNA Oligonucleotides

>> Optimised Application oligos

>> qPCR Probes Portfolio

Specifications

Unmodified RNA Oligos

What you can expect from our unmodified Custom RNA Oligos

Rely on first class RNA synthesis up to 80 bases

Minimum yields in OD
Sequence lengths:
- HPLC purified RNA: 5 - 80 bases
- Desalted RNA: 5 - 40 bases
Quality control by OD measurement and MALDI-TOF
Turnaround time: 8 - 10 working days
Delivery: lyophilised in 2ml screw cap tubes

Purification option	Purity	Synthesis scale [µmol]	0.05	0.20	1.00
Desalted	n.a.	Minimum yield [OD]	1.5	2	5
HPLC	> 85%	Minimum yield [OD]	0.5	2	2.5

Modified RNA Oligos

What you can expect from our modified RNA oligos

Product specifications:

Synthesis scales from 0.05 to 1.0 µmol
HPLC purified
Minimum yields in OD
Quality control by OD measurement and MALDI-TOF
Sequence lengths from 5 - 80 bases
Turnaround time: 8 - 10 working days
Delivery lyophilised in tube format

Purification option	Purity	Synthesis scale [µmol]	0.05	0.20	1.00
HPLC	>80%	Minimum Yield [OD]	0.5	2	2.5

Available modifications for our single strand custom RNA oligos:

Fluorescent dyes	5'	Int	3'
FAM [FAM]	x	-	-
TET [TET]	x	-	-
HEX [HEX]	x	-	-
Cyanine3 [CY3]	x	-	-
Cyanine5 [CY5]	x	-	-

Non fluorescent modifications	5'	Int	3'
Amino-C6 [AmC6]	x	-	-
Biotin [BIO]	x	-	-
Biotin-TEG [BIOTEG]	x	-	x
Phosphate [PHO]	x	-	x

Spacer, linker, base & sugar modifications	5'	Int	3'
2'-Deoxyinosine [I]	x	x	x
2'-Deoxyuridine [U]	x	x	x
5-Methyl-dC [5MedC]	x	x	x

O-Methyl-RNA / Chimerics

Chimeric oligos and 2'O-Methyl-RNA oligos

The use of 2'O-Methyl-RNA in chimeric antisense oligos increases nuclease stability of the oligo.

Chimeric oligonucleotides consist of mixed DNA and RNA bases including methylated bases or phosphorthioate bounds (PTO).

Product specifications:

HPLC purified
Synthesis scales from 0.05 µmol up to 1.0 µmol
Minimum yields in OD
Sequence lengths from 6 to 80 bases
Quality control by OD measurement and MALDI-TOF MS
TAT: 6 - 8 working days
Delivery: lyophilised in 2ml screw cap tubes

Synthesis scale [µmol]	0.05	0.20	1.00
Min. Yield [OD]	0.5	2	2.5
Length restriction [bases]	6 - 80	6 - 80	6 - 80

HPLC is required for purification of chimeric oligos. We recommend to perform a Na+ salt exchange with commercially available desalting columns (e.g. NAP or MicroSpin columns) before use in cells or live animals.

RNA qPCR Probes

What you can expect from our custom RNA qPCR Probes

RNA qPCR Probes are produced according to technologically advanced synthesis and purification protocols.

The range of reporter-quencher combinations suit probe-based hybridisation assays.

Product specifications: