Improve your next generation sequencing data
NGSgrade oligos validated by our NextGen sequencing lab
NGS adaptors and primers are crucial to the success of every next generation sequencing project. With projects normally costing thousands, oligos must deliver reliable performance.
Utilising outstanding expertise with long-year experience in next generation sequencing and oligonucleotide synthesis, Eurofins Genomics has now created the optimum NGSgrade oligos.
Our new NGSgrade Oligos reduce the risk of barcode misalignment during multiplex next generation sequencing.
- Guaranteed purity of > 80% by HPLC purification
- Specific cross-contamination free processes after synthesis
- Stringent quality criteria checked by MALDI-TOF MS
- Synthesis scales from 0.01 to 1.0 µmol
- Selectable delivery format: lyophilised and concentration adjusted
NGSgrade oligos are recommended for any kind of NGS application independent of the instrument or technology.
Select your required NGSgrade Oligos
Oligos for NGS amplicon sequencing with adaptor ligation approach
High quality adapter oligos to improve multiplexing with sample tagging by NGS on Illumina.
Eurofins Genomics' NextGen sequencing experts designed individual tags that we add to your target specific primer sequence. The resulting primers enable
optimised sequencing for sample pools with low and high amount of samples.
The tags are designed with a minimum edit distance of 4, which prevents in a best possible way mis-assignments in case of sequencing errors.
The individual tags consist of 10 nucleotides which will be added to each forward primer.
The designed primers can be used for our NGSelect Amplicon Adaptor Ligation product or with your own sequencing protocol.
Oligos for NGS amplicon sequencing with 2nd PCR approach
High quality PCR primers to improve multiplexing by NGS on Illumina.
When using the 2-step amplicon generation process for your NGS amplicon sequencing projects primers with universal adaptors are needed for the first PCR.
For your convenience, Eurofins Genomics is adding those adaptors to your target specific sequences automatically. Because of this automatical process during online ordering you can be sure to have the correct, validated and optimised design of your primers.
The designed primers can be used for our NGSelect Amplicon 2nd PCR service.
When ordering these oligos for sequencing in your own facility, please check compatibility of adaptors added with the indexing primers in your 2nd PCR step protocol.