What you can expect from our NGSgrade Oligos
Optimised and verified product specifications:
- Guaranteed purity of > 80% by HPLC purification
- Specific cross-contamination free processes after synthesis
- Stringent quality criteria checked by MALDI-TOF MS
- Synthesis scales from 0.01 to 1.0 µmol
- Selectable delivery format: lyophilised and concentration adjusted
Minimum yields (OD) per scale:
|
Synthesis scale
|
0.01 µmol
|
0.05 µmol
|
0.2 µmol
|
1.0 µmol
|
HPLC
|
Minimum Yield [OD] |
1 |
2 |
4 |
10 |
Additional online features free of charge
- Add a personal note to your oligo resp. plate
- Calculate the complementary sequence
- Select your preferred label layout for your oligo tubes
- Track production and delivery status online
- View your order history under "My Orders
Oligos for NGS amplicon sequencing with adaptor ligation approach
High quality adapter oligos to improve multiplexing with sample tagging by NGS on Illumina.
Eurofins Genomics' NextGen sequencing experts designed individual tags that we add to your target specific primer sequence. The resulting primers enable optimised sequencing for sample pools with low and high amount of samples.
The tags are designed with a minimum edit distance of 4, which prevents in a best possible way mis-assignments in case of sequencing errors. The individual tags consist of 10 nucleotides which will be added to each forward primer.
The designed primers can be used for our NGSelect Amplicon Adaptor Ligation product or with your own sequencing protocol.
Oligos for NGS amplicon sequencing with 2nd PCR approach
High quality PCR primers to improve multiplexing by NGS on Illumina.
When using the 2-step amplicon generation process for your NGS amplicon sequencing projects primers with universal adaptors are needed for the first PCR.
For your convenience, Eurofins Genomics is adding those adaptors to your target specific sequences automatically. Because of this automatical process during online ordering you can be sure to have the correct, validated and optimised design of your primers.
The designed primers can be used for our NGSelect Amplicon 2nd PCR service.
When ordering these oligos for sequencing in your own facility, please check compatibility of adaptors added with the indexing primers in your 2nd PCR step protocol.