INVIEW Human Exome

Clinical Research Exome - CRE V4

Clinical Research Exome - CRE V4.

The Agilent SureSelect Clinical Research Exome v4 is ideally suited to bridge the gap between Whole Exome Sequencing and Whole Genome Sequencing.

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Highlights

Discover more with 10x less compared to WGS
Pre-capture pooling for very cost-efficient enrichment
Post-capture pooling available on request
Whole Exome Sequencing plus
- non-coding pathogenic variants
- PGx profiling
- Mitochondrial genome
- and many more
Fully automated, under diagnostics standards (ISO 17025)

Design

Target size: 41.1 Mb
Design size 48.5 Mb
Coding content (CCDS Release 22, GENCODE V31, ReefSeq Release 95) = 100 %
Non-coding content
- ACMG 73 genes variants > 99.9%
- ClinVar P/LP variants 100%
- HGMD DM variants 100%

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Product Specifications & Ordering

Product portfolio

	CRE v4 from 30x (pre-capture)	CRE v4 110x (pre-capture)	CRE v4 from 30x (post-capture)
Agilent Kit	CRE V4	CRE V4	CRE V4
Capture size	41.1 Mb	41.1 Mb	41.1 Mb
Starting material	Various sources, but NO FFPE	Various sources, but NO FFPE	Various sources, but NO FFPE
Batch size	In batches of 8 samples	In batches of 8 samples	From single samples
On target coverage	Approx 30x	Approx 110x	Approx 30x
Additional coverage available	Yes (multiples of 10x)	No	Yes (multiples of 10x)
Raw data	4.05 Gb (+ 1.35 Gb / 10x)	14.85 Gb	4.05 Gb (+ 1.35 Gb / 10x)
Analysis of somatic mutations	++	++	+++
Analysis of germline mutations	+++	+++	+++
Delivery time	20 days up to 96 samples	20 days up to 96 samples	20 days for up to 32 samples
Express delivery time	9 days up to 24 samples 14 days up to 48 samples	9 days up to 24 samples 14 days up to 48 samples	9 days up to 24 samples 14 days up to 48 samples
Bioinformatics analysis available	Yes	Yes	Yes

Starting material

Accepted starting material

100 ng for Qubit quantified gDNA (up to 100 µl / concentration > 1 ng/µl), Qubit quantified
QC passed: if DQN ≥ 4.0, Threshold = 10.000 (gDNA); if DQN ≥ 2.0,

Please note, that only S1-classified material is accepted for DNA isolation ordered online. More information about current classification of biological material can be found here (TRBA 466). Please contact us for further information on DNA isolation from other starting material such as environmental or industrial samples or material classified as S2.

Sequencing specifications & capture protocol

2 x 150 bp paired-end sequencing mode on Illumina
Latest Agilent SureSelect Clinical Research Exome V4 Kit
Approx. 30x on target coverage
Flexible add-on coverage as multiples of 10x
Indexing before capture protocol (pre-capture) as well as post-capture available