Metabarcoding

Amplicon Metabarcoding

Metabarcoding is a DNA-based technique used to identify and analyze multiple species in a sample by sequencing specific genetic markers, known as barcodes. These barcodes are unique to each species, allowing for the detection of a wide range of organisms, including bacteria, fungi, plants, and animals, from environmental samples like soil, water, or air. The sequences are then compared to reference databases to provide a detailed profile of the species present, offering insights into biodiversity and ecosystem health.

 

 

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Amplicon Metabarcoding

 

 

Our metabarcoding service offers precise and highly sensitive identification and classification of a wide range of organisms. The service covers DNA amplification, library preparation, sequencing on Illumina's MiSeq platform, and advanced bioinformatics to deliver detailed insights into food safety, biodiversity and ecosystem health.

 

Highlights

 

We use specialized genetic markers for:

✔️ 16S for bacteria

✔️ cytB and COI for fish & seafood

✔️ Mitochondrial 16S for land vertebrates

✔️ 18S and ITS regions for fungi

✔️ trnL and ITS for plant identification

 

Next-generation sequencing for the identification of all species of the above categories in mixed samples

✔️ Guaranteed 60,000 reads for enhanced data quality

✔️ Services conducted in ISO 17025 certified laboratories

✔️ Comprehensive bioinformatics services

✔️ Data transfer via secure FT

  

Direct Online Ordering - Explore our NGS online shop
Metabarcoding—made to order

Our detailed NGS order page for Amplicon Metabarcoding for Species Determination includes all details you need to know about your product. 

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What types of samples can be tested?

Metabarcoding Service Portfolio

 

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Metagenomic Meat Authenticity

Metabarcoding analysis of food and feed samples by Next Generation Sequencing to detect all land vertebrate species present in a sample without necessary prior knowledge about expected species.

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Metagenomic Fish Authenticity

 

Metabarcoding analysis of food and feed samples by Next Generation Sequencing to detect all fishes present in a sample without necessary prior knowledge about expected species.

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Metagenomic Meat & Fish Authenticity

Metabarcoding analysis of food and feed samples by Next Generation Sequencing to detect all mammals AND fishes present in a sample without necessary prior knowledge about expected species.

 

 

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Fungi Authenticity by Metabarcoding

Metabarcoding analysis for authenticity control of edible fungi to detect fungi present in a sample without necessary prior knowledge about expected species.

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Metabarcoding for Invertebrates in Food Samples

Screening for invertebrates in food samples. Metabarcoding on invertebrates using massive parallel sequencing.

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Metabarcoding of environmental DNA (eDNA)

Environmental DNA analyses. Metabarcoding on invertebrates using massive parallel sequencing.

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Edible Plant Authenticity by Metabarcoding

Metabarcoding analysis for authenticity control of edible plants (plant species mixtures like spices, herbs, tea…) to detect plants present in a sample without necessary prior knowledge about expected species.

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Non-targeted Mould & Yeast Screening

Non-targeted mould and yeast screening of food and feed samples for microbial flora analysis (e.g. fermented products, ripening analysis, spoilage, pathogens), but also on environmental samples like soil, water and feces by Next Generation Sequencing.With this approach moulds and yeasts (also non-cultivable species) present in a sample can be identified in parallel without necessary pre-culturing by classical microbiology. All mould and yeast species of which a sequence is published in the NCBI nucleotide database can be identified.

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Non-targeted Microbiota Screening

Non-targeted microbiota screening of food and feed samples for microbial flora analysis (e.g. fermented products, ripening analysis, spoilage, pathogens), but also on environmental samples like soil, water and feces by NGS.

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Metabarcoding of Honey

We offer 4 different tests for honey testing. Identification of DNA from 

1. Plant species out of honey

2. Plant & Invertebrates species out of honey

3. Plant, Bacteria & Invertebrates species out of honey

4. Plant, Bacteria/Mould/Yeast & Invertebrates species out of honey

HOW DOES IT WORK?

6 easy steps to analyse your samples using Amplicon Metabarcoding

 

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Send in your sample

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DNA is extracted by Eurofins Genomics

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Library preparation

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Sequencing of the samples

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Bioinformatics analysis

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 Results uploaded to Ecom; confirmation email sent

Frequently Asked Questions

Please send your samples to:

Eurofins Genomics FEB
Anzingerstrasse 7
85560 Ebersberg

Germany

  • Please ensure to use free NGS Barcodes to label your samples
  • Please ensure that all samples are clearly and unambiguously labeled.
  • Print the order confirmation displayed at the end of the ordering process and enclose it with your shipment.
  • Please send between 2 g and 100 g of material, either cooled or frozen.

Species determination using Sanger / qPCR

 

 

For species determination using Sanger or qPCR, explore our extensive range of services on the dedicated order pages

Related Items

Sample shipment options

Order your UPS label or use our dropboxes for sample shipment. Please note that it is mandatory to use free NGS barcodes for sample shipment.

How to retrieve my results

Below you can find instructions how to access your FTP folder or share your results with your colleagues.

 

                    Quality is important for us at Eurofins 

 

Our products and services are produced and performed under strict quality management and quality assurance systems.

 

Find Certificates here