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    Header Image
     

     

    INVIEW Transcriptome

     

     

    Choose the perfect package for your application.

    Our NGS experts - working in this field since many years - defined the perfect products for your RNA-Seq project. Depending on sample source and sample amount you can choose the right package for you.

     

     

     

     

    >> Get Quote / Order

    >> Order Barcodes

    >> Order NGS Coupons

    >> Redeem NGS Coupons

     

     

     

    INVIEW Transcriptome Bacteria

     

    • High quality mapping results and gene expression profiles from prokaryotic samples.
    • Analyse specific genomic elements and their biological significance

    >> Learn more

     

    INVIEW Transcriptome Ultra Low

     

    • 0.15 ng/µl starting material are sufficient to perform your expression analysis experiment.

    >> Learn more

    INVIEW Transcriptome Discover

     

    • Discover and quantify novel transcripts and splicing variants
    • NGS-based RNA-seq of strand-specific libraries for profiling of rare, novel transcripts or for analysing splicing variants even without a reference sequence

    >> Learn more

     

     

     

     

    >> FAQ's Transcriptome

    >> NGS Prepaid Coupons

    >> Sample Submission

     

     

     

    Find your optimal INVIEW Transcriptome solution here

     

    Product Details

     

    Product
    Bacteria
    Discover
    Ultra Low
    Library Type Strand-specific cDNA libraries Strand-specific cDNA libraries Strand-specific cDNA libraries
    Starting material Prokaryoten Application for total RNA from various sources, starting from one sample Eukaryoten
    Sequencing technology Illumina Illumina Illumina
    Read Mode 150 bp paired-end reads 150 bp paired-end reads 150 bp paired-end reads
    Number of guaranteed reads Multiples of 10M read pairs Multiples of 30M read pairs Multiples of 5M read pairs
    Delivery time 15 working days for up to 192 samples 15 working days for up to 192 samples From 27 working days

    .

    Which product to choose

     

    Product
    Bacteria
    Discover
    Ultra Low
    Novel transcripts - + -
    Strand specificity information + + +
    Rare transcripts Illumina Illumina Illumina
    Read Mode Flexible increase of data output Flexible increase of data output Flexible increase of data output
    Splice variants n.a. + -
    Transcript identification + +++ +
    Differential expression + +++ +
    Mapping accuracy Sufficient Optimal Sufficient
    High quality reference Mandatory Additional Mandatory

    .

    Bioinformatics analysis

    • Semi-automatic mapping against one reference
    • Identification of potential exon-exon splice junctions
    • Identification and quantification of transcripts
    • Merging of identified pieces to full-length transcripts
    • Transcript annotation based on known annotations
    • Determination of expression levels
    • Pairwise comparison of expression levels and determination of significant fold differences
    • Detection and annotation of SNPs and InDels
    • Annotation of detected SNPs and InDels that are registered in dbSNP
    • Allocation of protein-level effects that are registered in Ensembl

     

     

    Deliverables

    • Alignment file (BAM)
    • Gene expression table including FPKM value (tsv)
    • Combined gene expression table of all samples (tsv)
    • Table of top genes expressed (tsv)
    • Table of pairwise differential gene expression including foldchange and p-value (tsv)
    • Table of genes expressed differently between pairs (tsv)
    • SNP and InDel tables including annotated variants and effects (vcf, tsv, bed)
    • Comprehensive Data Analysis Report (.pdf)

     

     

     

    Literature

     

    NGS RNA-Seq demo report

    NGS Genexpression profiling (human) demo report

    NGS Overview Flyer

     

     

     

     

     

     

     

    Solutions you may also like

     

     

     

    INVIEW Virus

     


    For all possible virus types

     


    >> READ MORE

     

     

     

    NGS Coupons

     


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    EVOCard

     


    The prepaid card for more
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    >> READ MORE

     

     

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