NGS Built For You

w10.0.13 | c1.0.0.25

PROD | u7.5.14

INVIEW Transcriptome

Choose the perfect package for your application.

Our NGS experts - working in this field since many years - defined the perfect products for your RNA-Seq project. Depending on sample source and sample amount you can choose the right package for you.

>> Get Quote / Order

>> Order Barcodes

>> Order NGS Coupons

>> Redeem NGS Coupons

INVIEW Transcriptome Bacteria

High quality mapping results and gene expression profiles from prokaryotic samples.
Analyse specific genomic elements and their biological significance

>> Learn more

INVIEW Transcriptome Ultra Low

0.15 ng/µl starting material are sufficient to perform your expression analysis experiment.

>> Learn more

INVIEW Transcriptome Discover

Discover and quantify novel transcripts and splicing variants
NGS-based RNA-seq of strand-specific libraries for profiling of rare, novel transcripts or for analysing splicing variants even without a reference sequence

>> Learn more

>> FAQ's Transcriptome

>> NGS Prepaid Coupons

>> Sample Submission

Find your optimal INVIEW Transcriptome solution here

Product Details

Product	Bacteria	Discover	Ultra Low
Library Type	Strand-specific cDNA libraries	Strand-specific cDNA libraries	Strand-specific cDNA libraries
Starting material	Prokaryoten	Application for total RNA from various sources, starting from one sample	Eukaryoten
Sequencing technology	Illumina	Illumina	Illumina
Read Mode	150 bp paired-end reads	150 bp paired-end reads	150 bp paired-end reads
Number of guaranteed reads	Multiples of 10M read pairs	Multiples of 30M read pairs	Multiples of 5M read pairs
Delivery time	15 working days for up to 192 samples	15 working days for up to 192 samples	From 27 working days

Which product to choose

Product	Bacteria	Discover	Ultra Low
Novel transcripts	-	+	-
Strand specificity information	+	+	+
Rare transcripts	Illumina	Illumina	Illumina
Read Mode	Flexible increase of data output	Flexible increase of data output	Flexible increase of data output
Splice variants	n.a.	+	-
Transcript identification	+	+++	+
Differential expression	+	+++	+
Mapping accuracy	Sufficient	Optimal	Sufficient
High quality reference	Mandatory	Additional	Mandatory

Bioinformatics analysis

Semi-automatic mapping against one reference
Identification of potential exon-exon splice junctions
Identification and quantification of transcripts
Merging of identified pieces to full-length transcripts
Transcript annotation based on known annotations
Determination of expression levels
Pairwise comparison of expression levels and determination of significant fold differences
Detection and annotation of SNPs and InDels
Annotation of detected SNPs and InDels that are registered in dbSNP
Allocation of protein-level effects that are registered in Ensembl

Deliverables

Alignment file (BAM)
Gene expression table including FPKM value (tsv)
Combined gene expression table of all samples (tsv)
Table of top genes expressed (tsv)
Table of pairwise differential gene expression including foldchange and p-value (tsv)
Table of genes expressed differently between pairs (tsv)
SNP and InDel tables including annotated variants and effects (vcf, tsv, bed)
Comprehensive Data Analysis Report (.pdf)