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Oligonucleotide Synthesis

Gene Synthesis & Molecular Biology

Sanger Sequencing

Next Generation Sequencing

Genotyping & Gene Expression

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INVIEW Transcriptome

 

 

Choose the perfect package for your application.

Our NGS experts - working in this field since many years - defined the perfect products for your RNA-Seq project. Depending on sample source and sample amount you can choose the right package for you.

 

 

 

 

 

 

 

INVIEW Transcriptome Bacteria

 

  • High quality mapping results and gene expression profiles from prokaryotic samples.
  • Analyse specific genomic elements and their biological significance

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INVIEW Transcriptome Ultra Low

 

  • 0.15 ng/µl starting material are sufficient to perform your expression analysis experiment.

>> Learn more

INVIEW Transcriptome Discover

 

  • Discover and quantify novel transcripts and splicing variants
  • NGS-based RNA-seq of strand-specific libraries for profiling of rare, novel transcripts or for analysing splicing variants even without a reference sequence

>> Learn more

 

 

 

 

 

 

 

Find your optimal INVIEW Transcriptome solution here

 

Product Details

 

Product
Bacteria
Discover
Ultra Low
Library Type Strand-specific cDNA libraries Strand-specific cDNA libraries Strand-specific cDNA libraries
Starting material Prokaryoten Application for total RNA from various sources, starting from one sample Eukaryoten
Sequencing technology Illumina Illumina Illumina
Read Mode 150 bp paired-end reads 150 bp paired-end reads 150 bp paired-end reads
Number of guaranteed reads Multiples of 10M read pairs Multiples of 30M read pairs Multiples of 5M read pairs
Delivery time 15 working days for up to 192 samples 15 working days for up to 192 samples From 27 working days

Which product to choose

 

Product
Bacteria
Discover
Ultra Low
Novel transcripts - + -
Strand specificity information + + +
Rare transcripts Illumina Illumina Illumina
Read Mode Flexible increase of data output Flexible increase of data output Flexible increase of data output
Splice variants n.a. + -
Transcript identification + +++ +
Differential expression + +++ +
Mapping accuracy Sufficient Optimal Sufficient
High quality reference Mandatory Additional Mandatory

Bioinformatics analysis

  • Semi-automatic mapping against one reference
  • Identification of potential exon-exon splice junctions
  • Identification and quantification of transcripts
  • Merging of identified pieces to full-length transcripts
  • Transcript annotation based on known annotations
  • Determination of expression levels
  • Pairwise comparison of expression levels and determination of significant fold differences
  • Detection and annotation of SNPs and InDels
  • Annotation of detected SNPs and InDels that are registered in dbSNP
  • Allocation of protein-level effects that are registered in Ensembl

 

 

Deliverables

  • Alignment file (BAM)
  • Gene expression table including FPKM value (tsv)
  • Combined gene expression table of all samples (tsv)
  • Table of top genes expressed (tsv)
  • Table of pairwise differential gene expression including foldchange and p-value (tsv)
  • Table of genes expressed differently between pairs (tsv)
  • SNP and InDel tables including annotated variants and effects (vcf, tsv, bed)
  • Comprehensive Data Analysis Report (.pdf)

 

 

 

Literature

 

 

 

 

 

 

 

 

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