Oxford nanopore (ONT) sequencing

Whole Genome sequencing with long read sequencing. Suitable for large fragment DNA samples. Novel Nanopore technology is used to obtain very long sequences of several kilobases.

Applications

• Resequencing of whole genomes
• Assembly of genomes
• Identification of taxonomic background
• Metagenomic analysis of long reads

Highlights

• Long read recovery of several kilobases
• Available from one sample
• 5 - 4 million reads per Flow Cell (dependent on DNA quality and length)

Product description:

• Library preparation:
  
  • Fragmentation
  • ONT adaptor ligation (multiplexing depends on genome size)
    
    
•  Sequencing:
  • Technology: Oxford Nanopore
  • Output read length: Equal to fragment length (can be up to several kbp)
  • Throughput is dependent upon the quality of sample submitted
  • Approx. read output: 0.5 - 4 M reads per Flow Cells (dependent on DNA quality and length
    
    
• Deliverables:
  • Raw Nanopore sequencing data (FastQ)
  • De novo assembled contigs (FASTA)
    
    
• Input required (dependent on multiplexing):
  • OD 260/280: 1.8
  • OD 260/230: 2.0-2.2
  • For 1 sample: Optimal: >100 ng/μl in 60 μl
  • For 2-5 samples: Optimal: >100 ng/μl in 30 μl
  • For 6-8 samples: Optimal: >100 ng/μl in 15 μl

Microbiome or community analysis with16S rRNA full length sequencing with Oxford Nanopore sequencing technology. Higher taxonomic resolution through availability of the whole 16S rRNA gene.

Applications

• Characterization of strains or communities
• Identification of taxonomic background
• Metagenomic analysis of long reads

Highlights

• Higher taxonomy resolution through long fragments unavailable with short read sequencing
• Available for 2-48 samples
• 5 - 4 million reads per Flow Cell (dependent on DNA quality and length)

Product description:

• Library preparation:
  
  • Full-length 16s rRNA gene sequencing using PCR amplification with primer pairs 27F and 1492R
  • Native barcoding + adapter ligation (up to 96 samples)
    
    
•  Sequencing:
  • Technology: Oxford Nanopore
  • Output read length: Equal to fragment length (for full-length 16S rRNA gene approx. 1700 bp)
  • Throughput is dependent upon the quality of sample submitted
    
    
• Deliverables:
  • Raw Nanopore sequencing data (FastQ)
  • Bacterial composition profiles (OTU tables)
    
    
• Input required (dependent on multiplexing):
  • > 2.5 ng/μl in 20 μl
  • OD 260/280: 1.8
  • OD 260/230: 2.0-2.2

Sequencing of large and/or repetitive amplicons.

Product description:

• Library preparation:
  
  • ONT adapter ligation (multiplexing depends on amplicon size and preferred coverage, max. 96 samples per Flow Cell)
    
    
•  Sequencing:
  • Technology: Oxford Nanopore
  • Output read length: Equal to fragment length (can be up to several kbp)
  • Throughput is dependent upon the quality of sample submitted
  • Approx. read output: 0.5 - 4 M reads per Flow Cells (dependent on DNA quality and length)
    
    
• Deliverables:
  • Raw Nanopore Amplicon data (FastQ)
  • BioIT on request (e.g. Variant analysis VCF-file)
    
    
• Input required (dependent on multiplexing):
  • OD 260/280: 1.8oOD 260/230: 2.0-2.2
  • Max. amplicon size: 20 kbFor 1 sample:
  • Optimal: >25ng/μl in 60 μlFor 2-8 samples:oSize range of different amplicons should be similar (+/-10%)
  • Optimal: >25ng/μl in 30 μl

Confirm your full plasmids faster, more accurately, and more affordably than ever before. NGS generation 3 technology has made it possible to quickly sequence whole plasmids in a fraction of the time and without the hassle of having to design and synthesize primers.

Applications

Whole plasmid sequencing using NGS Gen 3 technology is a powerful and accurate method for characterizing and analyzing plasmids, which makes it well suited for a wide range of applications.

• Plasmid Verification
• Resequencing of whole genomes
• Assembly of genomes
• Identification of taxonomic background
• Metagenomic analysis of long reads
• Much more

This product is coming up soon. If you are already interested in this service please send us your request >>