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EVOcard

Oligonucleotide Synthesis

Gene Synthesis & Molecular Biology

Sanger Sequencing

Next Generation Sequencing

Genotyping & Gene Expression

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INVIEW Transcriptome Ultra Low

 

 

Send us down to 0.15 ng/µl and receive high quality expression analysis results.

INVIEW Transcriptome Ultra Low is the best choice for all RNA-Seq projects with only a small amount of input RNA.

 

 

 

 

 

 

 

Applications

 

  • Gene expression analysis in tumor tissue down to single cell analysis
  • All RNA-Seq protocols where only a small amount of starting material is available

Highlights

 

  • Highly sensitive detection of new transcripts or splicing variants
  • Structural information from strand-specific reads
  • Flexible sequencing starting with 30 M guaranteed read pairs
  • Expert BioIT data analysis
  • Comprehensive data report with results presented in different formats
  • Applicable to samples from nearly any organism
  • Availability of reference sequence is not crucial
  • Optional services including RNA isolation and ribosomal RNA (rRNA) depletion

 

 

 

 

 

 

 

Product Specifications & Ordering

 

Starting material

Accepted starting material 

 

  • Total RNA from various sources
  • rRNA-depleted total RNA
  • At least 0.15 ng/µl, RIN > 6.5
  • Minimum 10 µl of total RNA

 

We also offer RNA isolation from various starting material.

Please note that only S1-classified material is accepted for RNA isolation ordered online. More information about the current rules for classifying biological material can be found here. Please contact us for further information on isolating RNA from material classified as S2.

Library types

Standard RNA library - oligo-dT priming, DNA synthesis, fragmentation & library preparation

Sequencing specifications

  • Illumina
  • 150 bp paired-end reads
  • 5 million read pairs guaranteed

 

Bioinformatics analysis

  • Semi-automatic mapping against one reference
  • Identification of potential exon-exon splice junctions
  • Identification and quantification of transcripts
  • Merging of identified pieces to full-length transcripts
  • Transcript annotation based on known annotations
  • Determination of expression levels
  • Pairwise comparison of expression levels and determination of significant fold differences
  • Detection and annotation of SNPs and InDels
  • Annotation of detected SNPs and InDels that are registered in dbSNP
  • Allocation of protein-level effects that are registered in Ensembl

 

 

Deliverables

  • Alignment file (BAM)
  • Gene expression table including FPKM value (tsv)
  • Combined gene expression table of all samples (tsv)
  • Table of top genes expressed (tsv)
  • Table of pairwise differential gene expression including foldchange and p-value (tsv)
  • Table of genes expressed differently between pairs (tsv)
  • SNP and InDel tables including annotated variants and effects (vcf, tsv, bed)
  • Comprehensive Data Analysis Report (.pdf)

 

Delivery time

27 days for up to 24 samples
30 days for up to 50 samples
33 days for up to 100 samples

 

 

Literature

 

 

 

 

 

 

 

 

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INVIEW Transcriptome Discover


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EVOCard

 


The prepaid card for more
flexibility in your research


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Quality is important for us

Our products and services are produced and performed under strict quality management and quality assurance systems.

Find certificates here