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    Next Generation Sequencing
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    INVIEW CRISPR Check


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    Adapt. lig. Oligos
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    Analysis of CRISPR induced mutations with high coverage and reliability

    INVIEW CRISPR Check is a uniquely designed service for NGS based analysis of mutations introduced by the CRISPR/Cas9 System. Specifically the INVIEW is suited for the detection of small InDels introduced by the Non-Homologous End Joining (NHEJ) repair system. Our INVIEW starts from amplicons generated in your lab that span the targeted locus and offers the complete bundle from library preparation, sequencing and bioinformatics analysis.

    The INVIEW CRISPR Check is offered in two variants that differ in the way you are preparing the amplicons:

    Adaptor Ligation Protocol:

    • You prepare individual PCR products without molecular tags or a pool of several tagged PCR products
    • Eurofins Genomics ligates sequencing adaptors for Illumina sequencing
    • Optionally: Free primer design starting from target specific sequences

    2nd PCR Protocol:

    • You prepare amplicons from your samples with specific universal overhangs
    • Eurofins continues with a 2nd PCR step, introducing index sequences for sample discrimination and adaptors for sequencing on Illumina
    • Optionally: Free primer design starting from target specific sequences

     


    (click to enlarge)

    Applications

    • Evaluation of CRISPR/Cas9 induced mutations in target genes with high sequence coverage and low limit of detection (LOD)
    • Screening of CRISPR/Cas9 edited samples for promising candidates
    • Evaluation of a newly developed editing system by analysing mutation efficiency and type of mutations
    • Analysis of on-target and / or (predicted) off-target loci for exclusion of side-effects

     

    Highlights of INVIEW CRISPR Check

    • Well suited for every lab setup and preference: Two amplicon library protocols are offered with different sample preparation requirements
    • Comprehensive reporting of wildtype and alternate allele sequences including their quantities
    • Editing efficiency for each sample and target site

     

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