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Benefit from more than 25 years of experience in oligonucleotide synthesis!
Optimise your research and save time with high quality gene synthesis and molecular biology services.
Hiqh quality Sanger sequencing with highest flexibility for every sample type.
NGS from experts - ISO-certified, fully automated and easy to order online.
Cell line authentication, Mycoplasmacheck, Fragment length analysis & tailored projects.
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Our genomics solutions support you along your drug development chain of small and large molecules and in precision medicine.
Benefit from our range of tailored, high throughput genotyping solutions to help you achieve your goals faster, for less.
Welcome to your full-service laboratory. Benefit from our end-to end solutions for sample collection kit logistics and genomic solutions.
DNA-based solutions to improve and support your analysis, monitoring and traceability across your value chain.
Our scalable and high-quality oligonucleotides synthesis offer makes us an ideal partner for your industry applications.
For your research project in academic, governmental and industrial environment we have the right genomic service.
Eurofins GenomicsGermany GmbHAnzinger Str. 7a85560 EbersbergGermany
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Oligonucleotide Synthesis
Gene Synthesis & Molecular Biology
Next Generation Sequencing
Genotyping & Genexpression
Primers & Probes for qPCR Applications
Oligos for Next Generation Sequencing
Primers for Sanger Sequencing
Oligos for Cloning Applications
Custom Oligos
Oligo Tools
Synthetic genes
GeneStrands
Molecular Biology Services
Sanger Sequencing Services
Prepaid Products for Sanger Sequencing
Additional Services
Genome Sequencing
Transcriptome Sequencing
Metagenome / Microbiome
Exome Sequencing & Oncology Solutions
CRISPR & Prepaid NGS Coupons
VIRUS
Plasmid Sequencing
Amplicon sequencing & Ready-to-Load
Oxford Nanopore projects (WGS, Amplicons, 16S)
ONT Lite
Mycoplasmacheck
CLA & FLA
Others
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Single-internal-quenched qPCR probes featuring enhanced quenching properties
Discover the perfect solution for your industrial needs with our Ultimate Precision Probes, featuring a single internal quencher.
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Your challenges
Do any of these sound familiar?
Ultimate Precision Probes solve it!
To address these common challenges, Eurofins Genomics introduces the optimised Ultimate Precision Probes.
The new design with a single internal quencher combined with the recently developed HPLC purification method enables highly reliable, accurate and robust qPCR assay performance.
While traditional dual-labelled probes typically have about 20 bases between the fluorescent dye and the quencher, the Ultimate Precision Probes reduce this distance to only 9 bases. The resulting stronger FRET effect leads to a significant improvement in the quenching of background fluorescence in qPCR.
IP-Friendly Dye & Quencher Portfolio
Ultimate Precision Probes cover the entire visible spectrum from blue to red wavelengths, for maximum flexibility in assay design. Simultaneously, all dyes and quenchers presented here are available for commercial use in diagnostic kits under IP-friendly conditions.
We look forward to a detailed consultation to plan a pilot project tailored to your needs. Please contact us!
Quenching efficiency is generally the better the closer the fluorophore and quencher are to each other spatially and the better the emission wavelength of the fluorophore matches the absorption maximum (Amax) of the quencher. Quenching range can differ compared to the displayed depending on the individual oligo sequence, and quencher / fluorophore combination due to other physical effects like contact quenching. All wavelength information is given in nm.
Related Information
What you can expect from our Ultimate Precision Probes
Optional services:
Please note, that Ultimate Precision Probes are recommended and optimised for qPCR analysis. For genotyping assays we recommend our Dual Labeled Probes.
In qPCR experiments, Ultimate Precision Probes show reduced background and increased normalised fluorescence compared to standard dual-labelled probes (representative data in Figure 1A and B). In particular, the Ultimate Precision Probes show higher endpoint fluorescence after normalisation and often earlier Ct values, thereby increasing the accuracy and the robustness of the qPCR assay.
More impressively, Ultimate Precision Probes with internal BHQ1 and BHQ2 have such strong quenching efficiencies that performance is comparable to their double-quenched derivatives. But compared to probes with two quenchers, the complexity in manufacturing and analysis and the associated additional costs are reduced with the Ultimate Precision Probes.
All our Ultimate Precision Probes are purified using our newly developed IP-RP HPLC method with optimised solvent systems. This also contributes to a reduced background signal as the baseline separation of the target oligonucleotide from its by-products strongly increase the purification efficiency.
For more information on the new Ultimate Precision Probes, please see the corresponding application note.
Figure 1. Improved quenching efficiencies using Ultimate Precision Probes with a single internal quencher: Amplification curves of qPCR results from dilution level 6 (104) of the primer concentration assay for target region N in our SARS-CoV-2 model system. All Probes labeled with 5’-FAM fluorophore and BHQ1 using different probe layouts. (A) Amplification plot without background correction (raw data), (B) amplification plot with background correction (normalized data). Primer concentration (760 nmol/L); blue line is standard probe with 3’-quencher; red line is double quenched probe; green line is Ultimate Precision Probe and black line is double quenched competitor probe (mean of three experiments). All reactions were performed using a Roche LightCycler 480 using the LightCycler 480 data analysis software (cf. Roche, 2008).
Literature
Application Note
Quality is important for us at Eurofins
Our products and services are produced and performed under strict quality management and quality assurance systems.