Plasmid Präparation

Eurofins Genomics’ plasmid preparation services are available from Midi prep to Giga prep scale resulting in µg to up to 20 mg of high-quality plasmid DNA. Preps starting from Mega prep are always processed endotoxin free and sterile. The Maxi preps can be prepared endotoxin free and sterile if desired. Animal product free preparation is also available for preps starting from Maxi prep.

Additionally, we also offer detection of endotoxin level (LAL test) as well as bioburden (sterility) assay.

Plasmid DNA preparation services are required in research laboratories, biotech and pharmaceutical companies for applications such as:

• Transfection into mammalian cells
• Production of viral vectors
• Production of antibodies and recombinant proteins
• Production of DNA vaccines
• Cell and gene therapy studies
• Preclinical studies
• Toxicological studies

Expected quantities

Scale	Culture Volume	Expected Quantity*
Midi Prep	50 - 100 ml	15 - 50 µg
Maxi Prep	100 - 250 ml	100 - 500 µg
Mega Prep	0.5 - 2.5 l	500 µg - 2.5 mg
Giga Prep	2.5 l	2.5 - 10 mg
Giga Prep Plus	5 l	5 - 20 mg

*Expected quantity for high-copy plasmids; the actual yield will vary for low- or medium-copy plasmids. Other factors may also decrease the yield (e.g. growth temperature, bacterial host strain, etc.)


Additional services

• DNA sequencing (1 read using a standard sequencing primer) **Free of charge**
• Restriction enzyme digestion
• Quality control report

Please note: if no quality control option is selected, then NO quality control will be performed.


Animal-product free preparation

Our larger preps can be ordered as “Animal Product Free”. We have developed an optimised protocol to offer a fully BSE/TSE-free preparation. Certified "Animal-product free" material (e.g. LB agar, LB media, RNase, plasticware) is used for the production and specially trained staff performs the preparation in a separate laboratory area. The resulting plasmids, to the best of our knowledge, are free of animal products.

Turnaround times

TAT for plasmid prep depends on availability of the plasmid. Production can only start after we have received your plasmid and have run a Quality Control, usually one Sanger DNA sequencing read. After successful Quality Control the TAT is ~ 10 days for Midis-Giga and ~15 Days for Giga II. For large batches please inquire for available production slots before ordering.

TAT for plasmid prep services ordered in combination with gene synthesis is ~ 15 days.

More information can be found in our FAQ section

Risk group classification sheet

Please download the risk group classification sheet and send the filled form to genes-eu[at]genomics.eurofinseu.com after placing your order.