Industrial-grade NGS Oligos

What you can expect from our industrial-grade NGS Oligos

• Typical cross contamination rate of 0.01 - 0.05% determined by our NGS lab (see cross-contamination rate)
• Available minimum quantities of 4 nmol, 10 nmol and 25 nmol
• DNA sequence length from 15 - 120 bases
• Available modifications are PTO bounds, 2’-Desoxyinosine, 2’-Desoxyuridine and 5’-Phosphate
• Quality is ensured by OD measurement and ESI-MS (electrospray ionization mass spectrometry)
• A quality report, including the ESI-MS spectra, is provided online free of charge
• Selectable solvents are bidest water, TE buffer (10 mM Tris, 1 mM EDTA; pH=8) or Low EDTA Puffer (10 mM Tris, 0.1 mM EDTA pH = 8)
• Various customization options to comply with your industry requirements are available upon request:
  • Custom normalization
  • Mixing or pooling
  • Verification of cross-contamination rate by NGS
  • Production of oligos > 120 bases
  • Fast delivery times from 8 working days

For delivery in tubes:

• Oligos are supplied in 2ml screw cap tubes either lyophilized or in liquid form at the selected concentration
• Oligo tube labels can be defined with personal customer barcodes

For delivery in plates:

• Oligos are supplied in the selected 96well plate in liquid form at the defined concentration
• A minimum of 12 oligos per 96-well plate is required

The cross-contamination rate of 0.01 – 0.05% was determined by sequencing representative sets of Unique Dual Index (UDI) primer pairs using Illumina instruments at Eurofins Genomics’ European NGS sequencing facility. The rate given represents the typical total percentage of read pairs not matching the expected UDI combinations for a set of 96 UDI primer pairs. The corresponding Application Note shows a cross-contamination rate of 0.0014% for a set of 14 UDI primer pairs.

Please note that the total cross-contamination rate (percentage of incorrectly assigned read pairs) is influenced by the total amount of UDI primer pairs analysed in parallel. The more UDI primers are analysed, the higher the total cross-contamination can get, as more unexpected index combinations can be attributed.

In all cases, the actual cross-contamination of primers would be even lower as stated above. This is because the association of reads with other unexpected UDI primer pairs is a result of primer cross-contamination and index hopping during sequencing. Discriminating between these causes is not possible in this analysis setup.

Please note that Eurofins Genomics does not determine the cross-contamination rate for each individual set of NGS oligonucleotides produced and shipped.

Product Data

Eurofins Genomics uses a new and proprietary synthesis device to produce high performance NGS grade oligonucleotides. This equipment enables best-in-class coupling efficiencies, resulting in a high percentage of non-truncated, full-length oligonucleotides.

Figure 1. Theoretical purities for NGSgrade 2.0 oligonucleotides. (A) Plot of theoretical purities for different lengths of oligos applying a coupling rate of 99.6%. This average coupling rate was determined based on a large and representative sample of data points. (B) Example IP-RP-HPLC-chromatogram for a UDI adapter primer of 72 nucleotides, recorded at λ = 260 nm. A purity of 74.0% was measured for the target product. Analytical IP-RP UHPLC was performed on a Vanquish Horizon Duo system from ThermoFisher Scientific.