Eurofins Genomics is using a quality base calling software which examines the peaks around each base call to assign a quality score (Q) to each base call. Quality scores (Q) range from 4 to about 60, with higher values corresponding to higher quality. The quality scores are logarithmically linked to error probabilities, as shown in the following table:
Quality Score | Probability of a wrong base call | Accuracy of a base call |
Q 10 |
1 in 10 |
90 % |
Q 20 |
1 in 100 |
99 % |
Q 30 |
1 in 1.000 |
99.9 % |
Q 40 |
1 in 10.000 |
99.99 % |
Q 50 |
1 in 100.000 |
99.999 % |