There are several reasons why your sample might have failed:
- The starting material did not consist of circular DNA.
In our library preparation protocol, circular DNA is required as the starting material. If linear DNA is provided, it will result in sample fragmentation and subsequently lead to poor or no results.
- Insufficient DNA concentration in prepared samples.
The primary reason for this issue is the use of a Nanodrop device to measure DNA concentration. We strongly recommend employing a Qubit or a similar method instead.
- Presence of mixed plasmid species and/or fragmented genomic DNA or fragmented plasmids within the samples.
You may detect signs of this failure mode through a wide range of read lengths reported in the raw read length histogram.
- Contaminants in your samples like Ethanol. For more info please click here: https://community.nanoporetech.com/contaminants
To interpret your results you can also have a look at our troubleshooting guide. Click here >>