2024
Gene synthesis: Klingler F., Schlossbauer P., Naumann L., Handrick R., Hesse F., Neusüß C., Otte K., Developing microRNAs as engineering tools to modulate monoclonal antibody galactosylation, Biotechnology and Bioengineering
Gene synthesis: Dulyayangkul P. et al., Harvesting and amplifying gene cassettes confers cross-resistance to critically important antibiotics, PLOS Pathogens
Gene synthesis: Shintomi K et al., Recombinant cyclin B-Cdk1-Suc1 capable of multi-site mitotic phosphorylation in vitro, PLOS ONE
Gene synthesis: Capin et al, An engineered baculoviral protein and DNA co-delivery system for CRISPR-based mammalian genome editing, Nucleic Acids Research
Gene synthesis: Grafakou A., et al, Discovery of antiphage systems in the lactococcal plasmidome, Nucleic Acids Research
Gene synthesis: Dvořák, P., Burýšková, B., Popelářová, B. et al. Synthetically-primed adaptation of Pseudomonas putida to a non-native substrate D-xylose. Nat Commun
2023
Gene synthesis: Aguiar, T.Q., Oliveira, C., Domingues, L. (2023). Megaprimer-Based PCR to Synthesize Fusion Genes for Cloning. In: Domingues, L. (eds) PCR. Methods in Molecular Biology
Gene synthesis: Deletti, G., Green, S.D., Weber, C. et al. Unveiling an indole alkaloid diketopiperazine biosynthetic pathway that features a unique stereoisomerase and multifunctional methyltransferase. Nat Commun
Gene synthesis: Wu D., Gucwa M., Czub M., Cooper D., Shabalin I., Fritzen R., Arya S., Schwarz-Linek U., Blindauer C., Minor W., Stewart A.. Structural and biochemical characterisation of Co2+-binding sites on serum albumins and their interplay with fatty acids. Chemical Science
Gene synthesis: Tashiro, K., Segawa, T., Futami, T. et al. Establishment and characterization of a novel kidney cell line derived from the common bottlenose dolphin. In Vitro Cell.Dev.Biol.-Animal
Gene synthesis: Ishida Y, Koretsune T, Ishiuchi E, Teshima M and Ito M (2023) A magnesium transporter is involved in the cesium ion resistance of the high-concentration cesium ion-resistant bacterium Microbacterium sp. TS-1. Front. Microbiol.
Gene synthesis: Mamat U., Hein M., Grella D., Taylor C., Scholzen T., Alio I., Streit W., Huedo P., Coves X., Conchillo-Sole O., Gomez C., Gibert I., Yero D., Schaible U.. Improved mini-Tn7 Delivery Plasmids for Fluorescent Labeling of Stenotrophomonas maltophilia. Applied and Environmental Microbiology
Gene synthesis: Sauge-Merle, S.; Recuerda, M.; Beccia, M.R.; Lemaire, D.; Cherif, R.; Bremond, N.; Merola, F.; Bousmah, Y.; Berthomieu, C. Development of an Efficient FRET-Based Ratiometric Uranium Biosensor. Biosensors
Gene synthesis: Chronopoulou S., Tsochantaridis I., Tokamani M., Kokkinopliti K., Tsomakidis P., Giannakakis A., Galanis A., Pappa A., Sandaltzopoulos R. Expression and purification of human interferon alpha 2a (IFNα2a) in the methylotrophic yeast Pichia pastoris,
Protein Expression and Purification
2022
Gene synthesis: Di Nitto C, Neri D, Weiss T, Weller M, De Luca R. Design and Characterization of Novel Antibody-Cytokine Fusion Proteins Based on Interleukin-21. Antibodies. 2022
GeneStrands: Forrest, D., Warman, E.A., Erkelens, A.M. et al. Xenogeneic silencing strategies in bacteria are dictated by RNA polymerase promiscuity. Nat Commun
Gene synthesis: Liu et al. Bivalent single domain antibody constructs for effective neutralization of Venezuelan equine encephalitis. Scientific reports
Gene synthesis: Reimer et al. Engineering the amoeba Dictyostelium discoideum for biosynthesis of a cannabinoid precursor and other polyketides. Nature Biotechnology
SARS-CoV-2 research: Signorini et al. Longitudinal, virological, and serological assessment of hospitalized COVID-19 patients
SARS-CoV-2 research: Monteiro et al. Recovery of SARS-CoV-2 from large volumes of raw wastewater is enhanced with the inuvai R180 system. Journal of Environmental Management
SARS-CoV-2 research: Herrlein et al. Comparative Investigation of Methods for Analysis of SARS-CoV-2-Spike-Specific Antisera. Viruses
2021
SARS-CoV-2 research: Anna et. al., High seroprevalence but short‐lived immune response to SARS‐CoV‐2 infection in Paris
SARS-CoV-2 research: Rajasekharan et. al., Inhibitors of Protein Glycosylation Are Active against the Coronavirus Severe Acute Respiratory Syndrome Coronavirus SARS-CoV-2
SARS-CoV-2 research: Bechtold et. al., Rapid Identification of SARS-CoV-2 Variants of Concern Using a Portable peakPCR Platform
Gene synthesis: Marusic, et al., The expression in plants of an engineered VP2 protein of Infectious Bursal Disease Virus induces formation of structurally heterogeneous particles that protect from a very virulent viral strain
Gene synthesis: Shams, et al., Evaluation of the Binding Kinetics of RHEB with mTORC1 by In-cell and In vitro Assays
Gene synthesis: Unno, et al., Heterologous expression of cryptic biosynthetic gene cluster from Streptomyces prunicolor yields novel bicyclic peptide prunipeptin
Gene synthesis: Nakazato, et al., Targeted base editing in the plastid genome of Arabidopsis thaliana
Gene synthesis: Marine, et al., Towards unravelling the Rosette agent enigma: Spread and emergence of the co-invasive host-pathogen complex, Pseudorasbora parva-Sphaerothecum destruens
Gene synthesis: Johari, et al., Design of synthetic promoters for controlled expression of therapeutic genes in retinal pigment epithelial cells
Gene synthesis: Wang, et al., A glycoengineered antigen exploiting a conserved protein O-glycosylation pathway in the Burkholderia genus for detection of glanders infections
2020
Gene synthesis: Wong T.S., Tee K.L. (2020) Gene Cloning. In: A Practical Guide to Protein Engineering. Learning Materials in Biosciences. Springer, Cham
SARS-CoV-2 research: Ruiz-Arrondo., et al., Detection of SARS‐CoV‐2 in pets living with COVID‐19 owners diagnosed during the COVID‐19 lockdown in Spain: A case of an asymptomatic cat with SARS‐CoV‐2 in Europe
Gene synthesis: Ferrari et al., The allergen Mus m 1.0102: Cysteine residues and molecular allergology, Molecular Immunology
Gene synthesis: Fernandes et al., The dimerisable Cre recombinase allows conditional genome editing in the mosquito stages of Plasmodium berghei
2019
Gene synthesis: Abu-Halima M., et al., Differential expression of miR-23a/b-3p and its target genes in male patients with subfertility
Gene synthesis: Johari Y. B., et al., CHO genome mining for synthetic promotor design,
Gene synthesis: Sota M., et al., Efficient transposition of Tn4556 by alterations in inverted repeats using a delivery vector carrying a counter-selectable marker for Streptomyces
Gene synthesis: Jimmy S., et al., Discovery of small alarmone synthetases and their inhibitors as toxin-antitoxin loci
Gene synthesis: Liu J., et al., Polychromatic solar energy conversion in pigment-protein chimeras that unite the two kingdoms of (bacterio)chlorophyll-based photosynthesis
Gene synthesis: Tebo AG., et al., A plit fluorescent reporter with rapid and reversible complementation
2018
Gene synthesis: Jaubert, C. et al., RNA synthesis is modulated by G-quadruplex formation in Hepatitis C virus negative RNA strand
Gene synthesis: Fusco, F. A. et al., Biochemical charaterization of a thermostable endomannanase / endoglucanase from Dictyoglomus turgidum
Gene synthesis: Bernardini A. et al., The phosphorylatable Ser320 of NF-YA is involved in DNA binding of the NF-Y trimer
Gene synthesis: Amiri, M. M. et al., Hersintuzumab: A novel humanized anti-HER2 monoclonal antibody induces potent tumor growth inhibition
Gene synthesis: Fathi, S. et al., Design and construction of a new recombinant fusion protein (2b2t+EPC1) and its assessment for serodiagnosis of cystic echinococcosis
Gene synthesis: Brenner, N. et al., Validation of Multiplex Serology detecting human herpesviruses 1-5
Gene synthesis: Nolvak, H. et al., Reduction of antibiotic resistome and integron-integrase genes in laboratory scale photobioreactors treating municipal wastewater
Gene synthesis: Chow J., et al., The Thaumarchaeon N. gargensis carries functional bioABD genes and has a promiscuous E. coli deltabioH-complementing esterase EstN1
2017
Gene synthesis: Le, T, M. et al., Vectors for Expression of Signal Peptide-Dependent Proteins in Baculovirus/Insect Cell Systems and Their Application to Expression and Purification of the High-Affinity Immunoglobulin Gamma Fc Receptor I in Complex with Its Gamma Chain.
Gene synthesis: Mekonnen S. et al., O-5S quantitative real-time PCR: a new diagnostic tool for laboratory confirmation of human onchocerciasis
Gene synthesis: Wingen, M. et al., Novel Thermostable Flavin‐binding Fluorescent Proteins from Thermophilic Organisms
2016
Gene synthesis: Nolvak H., et al., Inorganic and organic fertilizers impact the abundance and proportion of antibiotic resistance and integron-integrase genes in agricultural grassland soil
Gene synthesis: Whitten MM., et al., Symbiont-mediated RNA interference in insects
Gene synthesis: Ohno M., et al., Acidic mammalian chitinase is a proteases-resistant glycosidase in mouse digestive system
Gene synthesis: Hoshiba T., Decellularized matrices as in vitro models of extracellular matrix in tumor tissues at different malignant levels: Mechanism of 5-fluorouracil resistance in colorectal tumor cells
Gene synthesis: Turner K.B., et al., Next-Generation Sequencing of a Single Domain Antibody Repertoire Reveals Quality of Phage Display Selected Candidates
Gene synthesis: Sudo Y., et al., Functional and Photochemical Characterization of a Light‐Driven Proton Pump from the Gammaproteobacterium Pantoea vagans
Gene synthesis: Scorsone E., et al., Major Urinary Proteins on Nanodiamond-Based Resonators Toward Artificial Olfaction
2015
Gene synthesis: Brieke, C. et al., Rapid access to glycopeptide antibiotic precursor peptides coupled with cytochrome P450-mediated catalysis: towards a biomimetic synthesis of glycopeptide antibiotics. Organic & biomolecular chemistry
Gene synthesis: Takeishi, R. et al., Epimerization at C-3′′ in Butirosin Biosynthesis by an NAD+-Dependent Dehydrogenase BtrE and an NADPH-Dependent Reductase BtrF. ChemBioChem
2014
Gene synthesis: Buettner, K. et al., Protein surface charge of trypsinogen changes its activation pattern. BMC biotechnology 14(1)
Gene synthesis: Chiwata, R. et al., None of the Rotor Residues of F 1-ATPase Are Essential for Torque Generation. Biophysical journal 106(10)
Gene synthesis: Holmberg, M. et al., A versatile bacterial expression vector designed for single-step cloning of multiple DNA fragments using homologous recombination. Protein expression and purification 98
Gene synthesis: Dietz O. et al., Characterization of the small exported Plasmodium falciparum membrane protein SEMP1. PLOS ONE 9(7): e103272
Gene synthesis: Canali E. et al., A high-performance thioredoxin-based scaffold for peptide immunogen construction: proof-of-concept testing with a human papillomavirus epitope. Nature Scientific Reports 4(4729)
2013
Gene synthesis: Mueller W. et al., Hierarchical organization of multi-site phosphorylation at the CXCR4 C Terminus, PLOS ONE 8(5): e64975
Gene synthesis: Van Noort, V., Bradatsch, B., Arumugam, M., Amlacher, S et al., Consistent mutational paths predict eukaryotic thermostability. BMC Evolutionary Biology, 13(1), 7
2012
Gene synthesis: Carciofi M. et al. Concerted suppression of all starch branching enzyme genes in barely produces amylose-only starch granules. BMC Plant Biology 2012, 12:223
Gene synthesis: Lejonklou M. et al. Accelerated Proliferation and Differential Global Gene Expression in Pancreatic Islets of Five-Week-Old Heterozygous Men1 Mice: Men1 Is a Haploinsufficient Suppressor. Endocrinology April 2012 (Genes purchase/synthesis: kinase complex-associated protein (Ikbkap)
Cloning: Ullrich T. et al. Competitive Reporter Monitored Amplification (CMA) - Quantification of Molecular Targets by Real Time Monitoring of Competitive Reporter Hybridization. PLoS ONE 7(4): e35438. (IPC gene for HIV- 1 and HIV- 2 respectively cloned into the pCR 2.1 TOPO® vector)
Gene synthesis: Ritari J. et al. Detection of Human Papillomaviruses by Polymerase Chain Reaction and Ligation Reaction on Universal Microarray. PLoS ONE 7(3): e34211. (HPV sequences were synthesised and cloned into plasmid vector)
Site-directed mutatgenesis: Esposito F. et al. Down-Regulation of the miR-25 and miR-30d Contributes to the Development of Anaplastic Thyroid Carcinoma Targeting the Polycomb Protein EZH2. Journal of Clincial Endocrinology & Metabolism May 1, 2012 vol. 97 no. 5 E710-E718
2011
Gene synthesis: Weber E. et al. Assembly of Designer TAL Effectors by Golden Gate Cloning. PLoS ONE 6(5): e19722
Gene synthesis: Gays F. et al. Comprehensive Analysis of Transcript Start Sites in Ly49 Genes Reveals an Unexpected Relationship with Gene Function and a Lack Of Upstream Promoters. PLoS ONE 6(3): e18475.