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    INVIEW Transcriptome -
    RNA Sequencing

    • You are here:
    • Next Generation Sequencing >
    • NGS Built For You >
    • INVIEW Transcriptome
     

     

    RNA sequencing

    Eurofins provides comprehensive RNA-Seq services that offer deep molecular insights into the transcriptomes of a variety of organisms. Our solutions enable precise profiling of all RNA species, including non-coding RNAs, and facilitate the analysis of both sense and antisense transcripts through the use of strand-specific libraries. Whether you're investigating gene expression levels, transcript structures, or other aspects of the transcriptome, our RNA-Seq services deliver high-quality, quantitative results.

     

    In addition to RNA sequencing, we offer RNA isolation services and a variety of library preparation protocols tailored to your specific project needs. These include stranded poly(A) selection methods and rRNA depletion for FFPE, poor-quality, and bacterial samples. For blood RNA, we provide globin depletion, and for projects with extremely limited RNA input, we offer special protocols that can work with as little as 120 pg/µL of RNA. With these flexible and optimized approaches, we ensure reliable, high-quality data for your research.

     

     

     

     

    INVIEW Transcriptome Bacteria

     

    Illumina NovaSeq 

     

    Obtain high quality strand specific mapping results and gene expression profiles for your prokaryotic samples.

    INVIEW Transcriptome Discover

     

    Illumina NovaSeq

     

    Find strand-specific RNA-Seq solutions for eukaryotic samples, including animals, plants, yeast or viruses

    INVIEW Transcriptome Ultra Low

     

    Illumina NovaSeq

     

    Ultra-low input RNA sequencing provides gene expression analysis of samples containing just a few cells.

    >> Learn more

    >> Learn more

    >> Learn more

     

    Features

     

    • Services are performed in ISO17025 certified labs in Europe

    • Fast turn-around times

    • Flexible data quantity options

    • Optional RNA extraction services available

    • Data transfer via secure FTP

     

    Wide Range of applications & services  

     

    Explore our comprehensive bioinformatics services designed to meet diverse research needs.

    Our offerings include data analysis, interpretation, and visualization tailored to your specific projects.

    Benefit from interactive analysis reports that provide clear insights, enabling you to make informed decisions quickly.

    Our expert team is here to support you every step of the way. Elevate your research with our cutting-edge bioinformatics solutions today! 

     

     

     

     

     

    >> FAQ's Transcriptome

    >> NGS Prepaid Coupons

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    Find your optimal INVIEW Transcriptome solution here

     

     

    Product Details

     

    Product
    Bacteria
    Discover
    Ultra Low
    Library Type Strand-specific cDNA libraries Strand-specific cDNA libraries Strand-specific cDNA libraries
    Starting material Prokaryoten Application for total RNA from various sources Eukaryoten
    Sequencing technology Illumina Illumina Illumina
    Read Mode 150 bp paired-end reads 150 bp paired-end reads 150 bp paired-end reads
    Number of guaranteed reads Multiples of 10M read pairs Multiples of 30M read pairs Multiples of 5M read pairs
    Delivery time 15 working days for up to 192 samples 15 working days for up to 192 samples From 27 working days
    Min sample number From 1 sample From 1 sample From 24 samples

    .

     

     

     

     

    Which product to choose

     

    Product
    Bacteria
    Discover
    Ultra Low
    Novel transcripts - + -
    Strand specificity information + + +
    Rare transcripts Illumina Illumina Illumina
    Read Mode Flexible increase of data output Flexible increase of data output Flexible increase of data output
    Splice variants n.a. + -
    Transcript identification + +++ +
    Differential expression + +++ +
    Mapping accuracy Sufficient Optimal Sufficient
    High quality reference Mandatory Additional Mandatory

    .

    Pre-sequencing options

    Dependent on your project layout it is recommended to select a pre-sequencing option to get the most out of your sequencing data. 

     

    Here you can see an overview of the different services:

     

     

    Pre-Sequencing Service
    This service includes
    You get data for
    Comment
    RNA-seq without PolyA or Depletion cDNA library generation of all present RNAs Total RNA You receive data for the total RNA including high abundant RNAs like rRNA (~90 % of total RNA) and specific mRNAs
    Poly-A-selection* Bead-based poly-A-selection of your samples (mRNA enrichment) Only mRNA This service targets polyA-tails (mRNA) in a total RNA sample. Therefore, only polyA containing fragments will be targeted and sequenced. mRNAs usually make up only 1 to 2 % of the total RNA.
    If your samples have poor quality (e.g. FFPE material), the mRNA may be degraded. This would result in mRNA fragments without polyA tails which then could not be targeted. If you expect poor quality you might get better data for your research question with selecting rRNA depletion
    rRNA depletion Bead-based rRNA depletion of your total RNA samples (HMR/Bacteria/Plant) Total RNA minus rRNA This service targets ribosomal RNA and will remove it from the total RNA. Usually, rRNA make up ~90 % of total RNA. All remaining RNAs, including mRNA, tRNA lncRNAs, etc. will be prepped and sequenced
    globin depletion Bead-based depletion of globin mRNA of your samples Total RNA minus globin mRNA This service targets globin mRNA and will remove it from the total RNA. The remaining RNAs will be prepped and sequenced. This service can be combined with: rRNA depletion or PolyA enrichment, a minimum of 48 samples per project is required
    Ultra low cDNA library generation of all present RNAs with an adapted protocol for samples with low RNA concentration Total RNA This service is intended for samples with a RNA concentration of 0.12-2.3 ng/µl. Because this service needs special handling, a minimum of 48 samples is required.

    .

     

    * only for eukaryotic samples

     

    Bioinformatics analysis

     

    All details about our bioinformatics pipeline can be found here >>

     

     

    Literature

     

    NGS RNA-Seq demo report

    NGS Genexpression profiling (human) demo report

    You'll get a demo PDF report, but your comprehensive analysis report will be in interactive HTML format.

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    INVIEW Virus

     


    For all possible virus types

     


    >> READ MORE

     

     

     

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    EVOCard

     


    The prepaid card for more
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