Für die DNA Probenvorbereitung ist es ausreichend ein qualitativ hochwertiges "säulen-basiertes" DNA Präparationskit zu verwenden. Je besser die DNA Qualität desto besser die Ergebnisse.
- Any extraction method that yields high quality, high purity, high molecular weight (HMW), double-stranded gDNA that is free of nicks, gaps, breaks, and contaminants is suitable for this sequencing service.
- Our in-house preferred methods for extraction are the Zymo® Quick-DNA Miniprep Plus Kit (with a lysozyme pre-treatment for gram positive samples) and Zymo® Quick-DNA Fungal/Bacteria Miniprep Kit.
- Other extraction methods that we and others have had success include: Wizard® Genomic DNA Purification kit, Qiagen® MagAttract HMW kit and Qiagen® DNeasy kit