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          Learn more

           

           

    • Genotyping & Genexpression
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        • Personalised Cancer Therapy
        • Revolutionising human-like-protein production
        • The Microbiome Of Cancer
        • All about biomarker discovery
    • Agrigenomics
      • Markets
      • Agrigenomics
        • Plant Breeder
        • DNA marker discovery
        • Marker-assisted selection
        • GRAS-Di®
        • Microbiome and metagenomics
        • Animal Breeder
        • DNA Marker discovery
        • Pathogen screening
        • Parentage testing
        • Genomic selection
        • Marker-assisted selection
        • Webshop - GenFarmEval
        • Favorite Content
        • Microarrays Accelerate Blue Biotechnology
        • How To Do NGS 50% Faster
        • NGS Portfolio
        • GenFarmEval.com

          Visit our Webshop for Farmers

          learn more

    • Consumer Genomics
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        • Sequencing Services
        • Genotyping
        • Epigenome Profiling
        • Microbiome Analysis
        • Shotgun Sequencing
        • Whole Genome Sequencing
        • Additional Services
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        • The End of Gene Doping
        • Home Genomics Testing
        • IVDR Compliance
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        • Food Testing
        • Food Authenticity
        • Meat Traceability
        • Pathogen Traceability
        • Cannabis and Hemp Testing
        • Environmental Testing
        • Non-targeted detection of organisms / species
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        • eDNA Tracker
        • Favorite Content
        • Determine the Source of Meat
        • Pine Nuts – Why Testing For Edibility Matters
    • Diagnostic Kit Producer
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        • The Future of RNA Applications
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ONT Lite Whole Plasmid Sequencing

 

 

 

Plasmid sequencing – The quality-focused process

 


Confirm your full plasmids faster, more accurately, and more affordably than ever before!

Harness the power of long-read nanopore sequencing for complete plasmid verification and analysis.

 

prepaid coupons

Order in Plates 

Order in Tubes

Sample Barcodes

 

The benefits at a glance

 

 

  • Exceptionally fast turn-around time starting from 1 day
  • Starting from 15 € per sample
  • Complete verification of the entire plasmid sequence in one run
  • Highest Accuracy using latest Oxford Nanopore chemistry and our proprietary bioinformatics pipeline
  • Comprehensive data report prepared for immediate use in grant applications and publications
  • Only purified plasmid DNA required; no sequencing primers
  • Complimentary sample pickup available via DropBoxes
  • Prepaid option available

 

NEW: Whole Plasmid Sequencing Premium Service for samples submitted in plates.

More details can be found below

 

 

 

>> FAQ's WPS Sequencing

>> Troubleshooting

>> how to redeem prepaid coupons

>> Sequencing Accessories

 

 

Related Information

 

 

Specifications

 

  • Library preparation (amplification-free, including minimal fragmentation); using the rapid barcoding v14 library prep chemistry from Oxford Nanopore Technologies
  • Sequencing using Oxford Nanopore GridION (flow cell R10.4.1) 
  • Bioinformatics included: 
    • Comprehensive data analysis report with plasmid map, read histogram, coverage plot, assembly statistics and QC (.HTML)
    • Consensus plasmid sequence (.FASTA) and incl. quality scores (.FASTQ)
    • Annotated plasmid sequence of all contigs in GENBANK format (.GBK) (import directly into your favourite software like Sanger's Artemis, SnapGene, Geneious Prime, Benchling, SeqBuilder Pro etc.)
    • Plasmid map (.PNG, .HTML)
    • Per-base clonal purity data with confidence score (.CSV)
    • Raw plasmid sequence reads that aligned to the assembled plasmid (.FASTQ)
    • Annotated feature table, including the nucleotide sequence of each feature (.CSV)
    • Interested in Pod5: please use our premium service (see below).  Your data will be securely delivered through your Ecom account 

 

 

Sample Preparation & Submission


Use the following concentration and volume for your samples:

Sample type
Size Category
Length
Concentration Min. Volume TAT*
Plasmid 

Regular 2.5 - 25  kbp 30 ng/µl 20 µl 1-2 days
Large 25 - 125 kbp 50 ng/µl 30 µl 5-7 days
XL 125 - 300 kbp 50 ng/µl 50 µl 5-7 days

Please note: Samples that do not meet our concentration requirement might fail in sequencing or lead to inaccurate assemblies (link to FAQ). No repetition will be performed on failed samples due to wrong concentrations adjustment.

*TAT = Turnaround time

 

Sample Requirements

 

  • Sample Type: Clonal, circular, double-stranded plasmid DNA at the minimum volume and at concentration specified.
  • Do not use NanoDrop for DNA quantification. Spectrophotometric methods are unreliable—please use a fluorometric method such as Qubit for accurate concentration measurement.
  • Resuspension buffer: Nuclease-free water or elution buffer (10 mM Tris, pH 8.5).
  • Purity (OD260/280): 1.8–2.0
  • Purity may be analyzed with Nanodrop or other spectrophotometric methods. Avoid ethanol contaminations – if using Qiagen miniprep kit, we recommend performing the optional PB buffer wash step to increase purity.

 

Format:

  • Barcode labelled 1.5 ml Eppendorf Safe-Lock tubes
  • Avoid sealing tubes with tape or parafilm
  • Label tubes with our free barcode labels and affix them horizontally for easy scanning

 

Shipment method:

  • Use nearest DropBox location for free sample pickup or select a courier service of your choice
  • Ensure secure transport by using our approved sample bags and boxes
  • Ship all samples to our Eurofins Genomics sequencing lab in Cologne, Germany

 

 

Premium Service

 

The WPS Premium Service guarantees exclusive use of a fresh flow cell dedicated entirely to your samples. To ensure maximum data integrity, the flow cell is discarded immediately after sequencing.

 

If your project demands strict data purity and zero risk of cross-contamination with other customer samples, we highly recommend choosing our Premium Service. This ensures complete isolation of your sequencing run on a dedicated single-use flow cell, fully eliminating the risk of index hopping (see FAQ section on “Index hopping”).

 

How can I order the Premium Service ?


The Premium Service is only available for samples submitted in plates. During order placement, please check the "Premium Service" checkbox on the order page. Please note that this service incurs an additional fee.

 

 

 

Literature

 

Whole Plasmid Sequencing sample submission guide

Whole Plasmid Sequencing demo report

Blog post: Whole Plasmid Sequencing in the fight against antibiotic resistance

 

Need more?

 

In addition to our highly automized high-throughput service we also offer customized Oxford Nanopore service.

 

Learn more here >>

 

 

 

 

 

 

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Fast sequencing of plasmid clones w/o DNA preparation


>> READ MORE

 

 

 

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All other applications using the ONT technology


>> READ MORE

 

 

 

ONT Lite portfolio



View the complete ONT Lite portfolio


>> READ MORE

 

 

 

Synthetic Genes


100% sequence verified &
ready-to-use

 

>> rEAD MORE

 

Quality is important for us at Eurofins

Our products and services are produced and performed under strict quality management and quality assurance systems.

Find certificates here

Quality is important for us

Our products and services are produced and performed under strict quality management and quality assurance systems.

Find certificates here
Contact Us

TECHNICAL SUPPORT

Phone: +49 7531 816068

Toll Free Phone Number: 00800-200 100 20

E-Mail: support-eu@genomics.eurofinseu.com

HOURS

Mon-Fri: 8 : 00 AM – 6 : 00 PM, ET

QUOTES, PRICING & SPECIAL REQUESTS

Quotes are submitted, reviewed, and accepted through the online quoting tool. Learn more.
Please direct inquires about pricing and special project requests to your sales representative.
General questions: support-eu@genomics.eurofinseu.com

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