ONT Lite Whole Plasmid Sequencing

Typical applications

Whole plasmid sequencing using third generation NGS Generation technology is a powerful and accurate method for characterizing and analyzing plasmids, which make it well suited for a wide range of applications.

• Plasmid Verification
• Fast check and comparison of plasmid clones
• Resequencing of whole plasmids without a reference

How does it work?

Novel Nanopore technology is used to obtain very long sequences of several kilobases. It is a powerful method for characterising and analysing plasmids, which are small, circular pieces of DNA that can replicate independently of the genome.

There are several key benefits to using ONT for whole plasmid sequencing:

• High throughput: ONT allows for high-throughput sequencing of multiple samples in a single run, making it ideal for large-scale studies and projects.
• Long read lengths: ONT generates long, continuous reads, which are particularly useful for sequencing large plasmids or for assembling complex plasmid structures.
• High accuracy: ONT has a high accuracy rate, making it suitable for a wide range of applications, including plasmid engineering, quality control, and the identification of genetic mutations.
• High resolution: ONT provides high-resolution data, allowing researchers to accurately identify and characterize even subtle differences in plasmid sequences.
• Versatility: ONT is suitable for a wide range of sample types, including low copy number plasmids, making it a versatile tool for plasmid analysis.

Use the following concentration and volume for your samples:

Please note: Samples that do not meet our concentration requirement might fail in sequencing or lead to inaccurate assemblies (link to FAQ). No repetition will be performed on failed samples due to wrong concentrations adjustment.

 
Please send your sample tubes according to the requirements below:

• Use 1.5 ml safe-lock tubes for your samples
• Please provide your samples either in nuclease-free water or elution buffer (10 mM Tris, pH 8.5)
• Please do not use Nanodrop for your concentration measurement. Spectrophotometric techniques are unreliable for the quantification measurement. Please use a fluormetric method, like Qubit.
• Do not tape or wrap tubes with parafilm. Safe-lock tubes offer perfect sealing and evaporation protection
• Label your sample tubes with our free barcode labels. Affix the label horizontally

How and where to ship your samples:

• Use either one of your nearest DropBox for free sample pick up or a courier of your choice
• You can convinently use one of our sample shipment options to send your samples
• To send your samples to us safely, make use of our sample bags and boxes
• Send your samples to our Eurofins Genomics sequencing lab in Cologne, Germany

Please ensure that your sample is free of any contaminants (e.g., ethanol) that could negatively affect your results. A complete list of contaminants can be found here: https://community.nanoporetech.com/contaminants 

The following files will be delivered for each plasmid sample

• Data analysis report in HTML format
• FASTQ raw data delivered which align to the consensus sequence
• Assembled plasmid sequence in FASTA format
• Annotated plasmid sequence in GENBANK format
• Raw plasmid sequence reads that aligned to the assembled plasmid as FASTQ files
• Annotated feature table, including the nucleotide sequence of each feature in TABLE format

No delivery of: Pod5 or FASTQ files that don't align.

If you are interested in Pod5 files and initial FASTQ files please contact us, regarding our premium service.