INVIEW CRISPR Check
progress bar
progress bar

Frequently asked questions
Contact us
Subscribe to newsletter
w10.0.14 | c1.25.03.1
PROD | u7.5.14
  Quick Order  0
Ecom-Admin
  • Markets
  • Products
  • Company
  • Contact
  • EN
  • Oligonucleotide Synthesis
  • Gene Synthesis & Molecular Biology
  • Sanger Sequencing
  • Next Generation Sequencing
  • Genotyping & Gene Expression

Further Information:

>> EVOcard

>> Product FAQs

>> SALE %

Application Oligos

  • qPCR Probes
  • Ultimate Precision Probes
  • PCR Primer
  • SeqPrimer
  • Cloning Oligos
  • NGSgrade Oligos 2.0
  • NGSgrade Oligos
  • NGS UDI Primer Sets
>> Show more

Custom Oligos

  • Custom DNA Oligos
  • Salt-Free Oligos
  • Large Scale Oligos
  • Custom RNA Oligos
  • Special Requests
>> Show more

Oligo Tools

  • Oligo Analysis Tool
  • PCR Primer Design Tool
  • Sequencing Primer Design Tool
  • qPCR Assay Design Tool
  • siRNA Design Tool
  • Prepaid Oligo Coupons
  • Excel Order Forms
>> Show more

Benefit from more than 25 years of experience in oligonucleotide synthesis!

>> Show all products

Gene Synthesis

  • Standard Genes
  • Express Gene
  • Gene Synthesis Project
  • GENEius
>> Show more

GeneStrands

  • GeneStrands
  • Express GeneStrands

Molecular Biology Services

  • Plasmid Preparation
  • CRISPR/Cas9
>> Show more

Optimise your research and save time with high quality gene synthesis and molecular biology services.

>> Show all products

Sequencing Services

  • Mix2Seq Service
  • LightRun Services
  • TubeSeq Supreme
  • PlateSeq Supreme
  • Direct Colony Sequencing
  • Whole Plasmid Sequencing
  • Primer Walking Service
>> Show more

Prepaid Products

  • Mix2Seq Kits
  • LightRun Barcodes
  • Barcode Labels & Coupons
  • PlateSeq Kits
  • PlateSeq Kit Colony
  • ONT Prepaid Coupons
>> Show more

Additional Services

  • Free Barcode Labels
  • Sequencing Accessories
  • Sequencing Primers
  • Sample Shipment
>> Show more

Hiqh quality Sanger sequencing with highest flexibility for every sample type.

>> Show all products

Top 5 Products

  • INVIEW Resequencing
  • INVIEW Microbiome
  • INVIEW Transcriptome
  • INVIEW Metagenome
  • Amplicon sequencing
  • Bioinformatic services
  • Genome sequencing
>> Show more

Spotlight

  • INVIEW Exome
  • INVIEW Virus
  • INVIEW CRISPR Check
  • NGS Prepaid Coupons
  • Oncology Solutions
  • OLINK Explore 3072
  • Clinical Research Exome
  • Bacteria Hybrid Assembly
>> Show more

Oxford Nanopore Products

  • Whole Plasmid Sequencing
  • Linear / Clonal Amplicons
  • Bacterial Genome Sequencing
  • 16S Full-Length Microbiome
  • Complete ONT Portfolio
>> Show more

NGS from experts - ISO-certified, fully automated and easy to order online.

>> Show all products

Applied Genomic Services

  • DNA Barcoding
  • Cell Line Authentication
  • Mycoplasmacheck
  • Fragment Length Analysis
>> Show more

Genotyping services

  • SNP Genotyping
  • Copy Number Variation
  • Mikrosatellites/ STR/FLA/ IDAA
>> Show more

Gene Expression Services

  • Transcriptome analysis
  • Expression Arrays
  • Target Gene Expression
>> Show more

Cell line authentication, Mycoplasmacheck, Fragment length analysis & tailored projects.

>> Show all products
  • Pharma / Biotech
  • Agrigenomics
  • Consumer Genomics
  • Food & Environment
  • Diagnostic Kit Producer
  • Research / Biotech

Further Information:

>> Quality

>> Events

Synthesis Products

  • Industrial-grade NGS Oligos
  • Ultimate Precision Probes
  • Special Oligo Requests
  • Large Scale Oligos
  • Gene Synthesis
  • GeneStrands
  • Plasmid Preparation

Sequencing Services

  • Amplicon Sequencing
  • Exome Sequencing
  • Transcriptome Sequencing
  • Primer Walking Service

Favorite Content

  • NGS – Disease Diagnostics
  • Cancer Ecology
  • Personalised Cancer Therapy
  • Revolutionising human-like-protein production
  • The Microbiome Of Cancer
  • All about biomarker discovery

Our genomics solutions support you along your drug development chain of small and large molecules and in precision medicine.

>> Show all products

Plant Breeder

  • DNA marker discovery
  • Marker-assisted selection
  • GRAS-Di®
  • Microbiome and metagenomics

Animal Breeder

  • DNA Marker discovery
  • Pathogen screening
  • Parentage testing
  • Genomic selection
  • Marker-assisted selection

Favorite Content

  • Microarrays Accelerate Blue Biotechnology
  • How To Do NGS 50% Faster
  • NGS Portfolio

Benefit from our range of tailored, high throughput genotyping solutions to help you achieve your goals faster, for less.

>> Show all products

Sequencing Services

  • Genotyping
  • Epigenome Profiling
  • Microbiome Analysis
  • Shotgun Sequencing
  • Whole Genome Sequencing

Additional Services

  • Sample Collection Kits
  • Shipping
  • DNA Extraction
  • Laboratory Service
  • Biobanking

Favorite Content

  • Population Genetics
  • The End of Gene Doping
  • Home Genomics Testing
  • IVDR Compliance

Welcome to your full-service laboratory. Benefit from our end-to end solutions for sample collection kit logistics and genomic solutions.

>> Show all products

Food Testing

  • Food Authenticity
  • Meat Traceability
  • Pathogen Traceability
  • Cannabis and Hemp Testing

Environmental Testing

  • Non-targeted detection of organisms / species
  • Targeted detection of organisms / species
  • eDNA Tracker

Favorite Content

  • Determine the Source of Meat
  • Pine Nuts – Why Testing For Edibility Matters

DNA-based solutions to improve and support your analysis, monitoring and traceability across your value chain.

>> Show all products

Synthesis Products

  • Large Scale Oligos
  • Special Requests in Tubes
  • Special Requests in Plates

Quality Assurance

  • GLP
  • ISO 17025
  • ISO 13485

Favorite Content

  • Oligonucleotides For Diagnostics
  • The Future of RNA Applications

Our scalable and high-quality oligonucleotides synthesis offer makes us an ideal partner for your industry applications.

>> Show all products

Favorite Services

  • Custom DNA Oligos
  • TubeSeq Services Sanger
  • INVIEW Microbiome NGS
  • NGS Services
  • GeneStrands

Express Services

  • TubeSeq NightXpress
  • Mix2Seq NightXpress
  • Express Genes
  • Express GeneStrands

Favorite Content

  • Eurofins Genomics Goes Green
  • GENEius – Codon Usage Optimisation
  • 5 tips to speed up your qPCR

For your research project in academic, governmental and industrial environment we have the right genomic service.

>> Show all products

Corporate Information

  • About us
  • Career
  • Events
  • Press Releases
  • Collaborations
  • Blog
  • Newsletter
  • Quality Assurance
  • Lab closure times

Help

  • Video Tutorials
  • Ordering
  • Payment
  • Shipping & Delivery
  • Downloads
  • Data & Privacy
  • Material and Methods

Contact us

Eurofins Genomics
Germany GmbH
Anzinger Str. 7a
85560 Ebersberg
Germany

  • phone +49 7531 816068
  • e-mail support

Get in touch with us!

You need support or advice with your order? You don't find the perfect product or you like to get consultation regarding your results?

>> Get in touch

Find your product

Find your product

  • Sequencing Primer
  • PCR Primer
  • Mycoplasmacheck
  • SALE %
  • TubeSeq

Login to Eurofins

Please login with your email address and password!

>> Login

New at Eurofins Genomics?

Register a new account at Eurofins Genomics!

>> Register
Impersonating: Max Mustermann

Administration

  • Ecom Admin
  • Stop Impersonating

Welcome

Julian Schlossmacher

Login / Register

 
Email
Email can not be empty
Password:
Password can not be empty
Forgot password?
Create Account
No SSL
>> Logout >> My profile

Contact

Dr. Melvin Siliakus

+31 629 39 25 66 >> Send message

Account

  • Overview
  • Orders
  • Quotes
  • EVOcards
  • Preferences
  • DropBoxes
  • Sanger Kits & Barcodes
  • NGS Barcodes & Coupons
  • Sanger Samples & Primers
  • Oligo Coupons

Language

model-logo

New Website Navigation explained

>> Close
6

Last added items

0 Item(s)

Go to Cart

>> Go to cart X
 

Quick Order

X

Industrial-grade Products & Services

What are industrial-grade products? <<click here >>

Oligonucleotide Synthesis

  • Ultimate Precision Probes
  • NGSgrade Oligos in Tubes
  • NGSgrade Oligos in Plates
  • Request for Oligos in Tubes
  • Request for Oligos in Plates
  • Large Scale Oligos

Gene Synthesis & Molecular Biology

  • Gene Synthesis
  • GeneStrands
  • Express GeneStrands
  • Plasmid Preparation
  • Cloning Service

Next Generation Sequencing

  • Genome (Re-)Sequencing
  • Transcriptome Sequencing
  • Microbiome profiling
  • Exome Sequencing
  • Clinical Research Exome
  • Oncoprofiling
  • Liquid Biopsy Sample Sequencing
  • Ready-to-Load
  • Virus Sequencing
  • Amplicon Sequencing
  • Metagenome
  • ONT products
  • NGS Barcodes & UPS labels
  • Replacement samples
  • NGS Additional Services

Genotyping & Genexpression

  • Cell Line Authentication
  • Fragment Length Analysis
  • Mycoplasmacheck Barcodes
  • Cell Line Barcodes

Oligonucleotide Synthesis

Primers & Probes for qPCR Applications

  • PCR Primer in Tubes
  • PCR Primer NightXpress
  • PCR Primer in Plates
  • LocNA Primer
  • Dual Labeled Probes
  • MGB Probes
  • LocNA Probe
  • Molecular Beacons
  • LightCycler Probes
  • Probe based qPCR Assay
  • Ultimate Precision Probes

Oligos for Next Generation Sequencing

  • NGSgrade Oligos 2.0 Tubes
  • NGSgrade Oligos 2.0 Plate
  • NGSgrade Oligos in Tubes
  • NGS Oligos for NovaSeq
  • NGS Oligos for MiSeq
  • NGS UDI Primer Sets

Primers for Sanger Sequencing

  • SeqPrimer in Tubes
  • SeqPrimer NightXpress
  • SeqPrimer in Plates
  • Standard Primer

Oligos for Cloning Applications

  • Cloning Oligos
  • EXTREmer Oligos

Custom Oligos

  • Custom DNA Oligos in Tube
  • SaltFree Oligo NightXpress
  • Custom DNA Oligos in Plates
  • Custom RNA Oligos
  • O-Methyl-RNA / Chimerics
  • RNA qPCR Probes
  • siMAX siRNA
  • Special Oligos in Tubes

Oligo Tools

  • Oligo Analysis Tool
  • PCR Primer Design
  • SeqPrimer Design
  • qPCR Assay Design
  • siRNA Assay Design
  • Prepaid Oligo Coupons

Gene Synthesis & Molecular Biology

Synthetic genes

  • Gene Synthesis
  • Combinatorial libraries
  • Gene Synthesis Projects

GeneStrands

  • GeneStrands
  • Express GeneStrands

Molecular Biology Services

  • Plasmid Preparation
  • Site Directed Mutagenesis
  • DNA Cloning Service

Sanger Sequencing

Sanger Sequencing Services

  • TubeSeq Supreme
  • PlateSeq Supreme
  • Direct Colony Sequencing
  • Ready2Load Plate
  • Ready2Load Tube
  • Whole Plasmid Sequencing Tubes
  • Whole Plasmid Sequencing Plate
  • TubeSeq NightXpress
  • Primer Walking Service

Prepaid Products for Sanger Sequencing

  • Prepaid Barcodes & Coupons
  • Mix2Seq Kits
  • PlateSeq Kits
  • LightRun Barcodes
  • ONT Coupons

Additional Services

  • Tube & Plate Barcode Labels
  • Sequencing Accessories
  • Sample Shipment
  • Sequencing Primer Design

Next Generation Sequencing

Genome Sequencing

  • INVIEW Resequencing
  • NGSelect DNA
  • Whole Genome Sequencing
  • Bact. Whole Genome Seq (ONT)

Transcriptome Sequencing

  • INVIEW Transcriptome
  • NGSelect RNA

Metagenome / Microbiome

  • INVIEW Microbiome
  • INVIEW Metagenome

Exome Sequencing & Oncology Solutions

  • INVIEW Human Exome
  • Liquid Biopsy Samples
  • INVIEW Oncoprofiling
  • Clinical Research Exome

CRISPR & Prepaid NGS Coupons

  • INVIEW CRISPR Check
  • Redeem NGS Coupons
  • Order NGS Coupons

VIRUS

  • INVIEW Virus

Plasmid Sequencing

  • INVIEW Plasmid Verification
  • Whole Plasmid Sequencing (ONT) Tubes
  • Whole Plasmid Sequencing (ONT) Plate

Amplicon sequencing & Ready-to-Load

  • NGSelect Amplicon
  • NGSelect Ready-to-Load
  • Clonal Amplicons (ONT)

Oxford Nanopore projects (WGS, Amplicons, 16S)

  • ONT projects

ONT Lite

  • ONT Lite Whole Plasmid Sequencing
  • ONT Lite Clonal Amplicon Sequencing
  • ONT Lite Bacterial Genome Sequencing
  • ONT Lite Assembly Review

Additional Services

  • NGS Barcodes & UPS labels
  • NGS Additional Services
  • Sample Submission Guidelines
  • Prepaid NGS Coupons
  • Replacement samples
  • Request for Information

Genotyping & Gene Expression

Mycoplasmacheck

  • Mycoplasmacheck Barcodes
  • Mycoplasmacheck results

CLA & FLA

  • Cell line authentication Service (CLA)
  • Fragment length Analysis (FLA)
  • Cell line authentication barcodes

Others

  • Genotyping request form

EVOcard

Order / Refill EVOcard

Login / Register

 
Email
Email can not be empty
Password:
Password can not be empty
Forgot password?
Create Account
No SSL

 

  • Order Menu

    EVOcards

    • Order / Refill EVOcard

    Oligonucleotides & siRNA

    • (q)PCR Primer in Tubes
    • (q)PCR Primer in Plates
    • (q)PCR Primer NightXpress
    • SeqPrimer in Tubes
    • SeqPrimer in Plates
    • SeqPrimer NightXpress
    • Custom DNA Oligos in Tubes
    • Custom DNA Oligos in Plates
    • SaltFree Oligo NightXpress
    • NGSgrade Oligos in Tubes
    • Standard Primer
    • Standard Primer NightXpress
    • LocNA Primer
    • LocNA Probes
    • Dual Labeled Probes
    • MGB Probes
    • Probe based qPCR Assay
    • Cloning Oligos in Tubes
    • EXTREmer Oligos
    • Nano-Scale Plate Oligos
    • NGS UDI Primer Sets
    • Custom RNA Oligos
    • RNA qPCR Probes
    • siMAX siRNA
    • Large Scale Oligos
    • Special Oligo Requests
    • More...

    Custom DNA Sequencing

    • Mix2Seq Kits
    • LightRun Barcodes
    • Sequencing Primers
    • TubeSeq Service
    • SupremeRun Tube
    • Tube & Plate Barcode Labels
    • TubeSeq Labels & Coupons
    • SupremeRun Barcodes
    • Sequencing Accessories
    • PlateSeq Kits
    • SupremeRun 96
    • Primer Walking
    • PlateSeq Service
    • SupremeRun | Multiprimer
    • Sequencing Projects
    • Direct Colony Sequencing
    • Ready2Load Plate
    • More...

    Next Generation Sequencing

    • INVIEW Microbiome 3.0
    • NGSelect DNA
    • Barcode & UPS Labels
    • INVIEW Transcriptome
    • NGSelect RNA
    • Replacement samples
    • INVIEW Resequencing
    • NGSelect Amplicon
    • Sample Submission
    • INVIEW Human Exome
    • Redeem NGS Coupons
    • Request for Information
    • INVIEW CRISPR Check
    • SARS-CoV-2 RNA-Seq
    • More...
    • INVIEW Virus

    Gene Synthesis & Molecular Biology

    • New gene wizard
    • Plasmid Preparation
    • GeneStrands
    • Express Genes
    • DNA Cloning Service
    • Express GeneStrands
    • Gene Synthesis Project
    • Site Directed Mutagenesis
    • Combinatorial libraries
    • Synthetic genes
    • Corona Control Plasmids

    Genotyping & Gene Expression

    • Mycoplasmacheck
    • Cell Line Authentication 2.0
    • Fragment Length Analysis
    • Request for Information

0 Item(s)

Go to Cart

  • Other Languages
  • DNA & RNA
    Oligonucleotides
    • >

      Optimised Application Oligos

    • >
      Ultimate Precision Probes
    • >
      PCR Primers
    • >
      SeqPrimer
    • >
      Cloning Oligo
    • >
      EXTREmers
    • >
      NGSgrade Oligos
    • >

      qPCR Probes

    • >
      NGSgrade Oligos 2
    • >
      Custom DNA & RNA Oligos
    • >
      Custom DNA Oligos
    • >
      Large Scale Oligos
    • >
      Custom RNA Oligos
    • >
      siMAX siRNA
    • >
      Special Requests
    • >
      Salt Free Oligos
  • Custom DNA Sequencing
    • >

      Eurofins Services

    • >
      Mix2Seq
    • >
      Mix2Seq Kits
    • >
      TubeSeq Services
    • >
      TubeSeq Labels & Coupons
    • >
      PlateSeq Service
    • >
      PlateSeq Kits
    • >
      Direct Colony Sequencing
    • >
      Whole Plasmid Sequencing
    • >

      LightRun Services

    • >
      LightRun Barcodes
    • >
      GATC Services
    • >

      Additional Services

    • >
      Tube & Plate Barcode Labels
    • >
      Sequencing Primers
    • >
      Sequencing Accessories
    • >
      Sample Shipment
    • >
      Free Sample Pick-Up
    • >
      Shipping Options
  • Next Generation Sequencing
    • >

      NGS Built For You

    • >
      INVIEW Microbiome
    • >
      INVIEW Transcriptome
    • >
      INVIEW Exome
    • >
      INVIEW Metagenome
    • >
      INVIEW CRISPR Check
    • >
      INVIEW Virus
    • >
      NGS prepaid solutions
    • >
      INVIEW Plasmid Verification
    • >

      NGS Build Your Own

    • >
      NGSelect DNA
    • >
      NGSelect RNA
    • >
      NGSelect Amplicons
    • >
      NGSelect Ready2Load
    • >
      Human Whole Genome Sequencing
    • >
      WGS Select
    • >
      Bacteria Whole Genome Sequencing
    • >
      Amplicon sequencing
    • >
      Amplicon sequencing - full service
    • >

      Applications

  • Gene Synthesis / Molecular Biology
    • >

      Gene Synthesis

    • >
      Standard Genes
    • >
      Express Genes
    • >
      Complex Genes
    • >
      GeneStrands
    • >
      Express GeneStrands
    • >
      Combinatorial Libaries
    • >
      GENEius
      • >

        Molecular Biology Services

      • >
        Plasmid Preparation
      • >
        Site Directed Mutagenesis
      • >

        Applications

      • >
        CRISPR_Cas9
    • Genotyping & Gene Expression
      • >

        Service Platforms

      • >
        Illumina Array Platforms
      • >
        Affymetrix Array Platforms
      • >
        Fluidigm BioMark & EP1
      • >
        Roche LightCycler
      • >
        Sanger & NGS Sequencing
      • >

        Genotyping Services

      • >
        SNP Genotyping
      • >
        Microsatellites / STR / FLA / IDAA
      • >
        Sequencing Based Genotyping
      • >
        Genotyping by PCR
      • >
        Genome Wide Association
      • >
        Human Identification
      • >
        Copy Number Variation
      • >

        Gene Expression Services

      • >
        Transcriptome Analysis
      • >
        Expression Arrays
      • >
        Target Gene Expression
      • >
        miRNA / Small RNAs Analysis
      • >

        Applied Genomics Services

      • >
        Residual DNA Analysis
      • >
        DNA Barcoding
      • >
        Cell Line Authentication
      • >
        Mycoplasmacheck

    CRISPR analyzer

    • You are here:
    • Next Generation Sequencing >
    • NGS Built For You >
    • INVIEW CRISPR Check
     

     

    CRISPR analyzer – Evaluate CRISPR/Cas-9 Induced-Effects.

    INVIEW CRISPR Check is a uniquely designed service for NGS based analysis of mutations introduced by the CRISPR/Cas9 System. Specifically the INVIEW is suited for the detection of small InDels introduced by the Non-Homologous End Joining (NHEJ) repair system. Our INVIEW starts from amplicons generated in your lab that span the targeted locus and offers the complete bundle from library preparation, sequencing and bioinformatics analysis.

     

     

    Get Quote / Order

    Order Barcodes

    Order NGS Coupons

    >> Redeem NGS Coupons

     

    Applications

     

    • Evaluation of CRISPR/Cas9 induced mutations in target genes with high sequence coverage and low limit of detection (LOD)
    • Screening of CRISPR/Cas9 edited samples for promising candidates
    • Evaluation of a newly developed editing system by analysing mutation efficiency and type of mutations
    • Analysis of on-target and / or (predicted) off-target loci for exclusion of side-effects

     

     

    Highlights of INVIEW CRISPR Check

     

    • Well suited for every lab setup and preference: Two amplicon library protocols are offered with different sample preparation requirements
    • Comprehensive reporting of wildtype and alternate allele sequences including their quantities
    • Editing efficiency for each sample and target site

    The INVIEW CRISPR Check is offered in two variants that differ in the way you are preparing the amplicons

     

    INVIEW CRISPR Check (150 - 270 bp)

     

    • You prepare individual PCR products without molecular tags or a pool of several tagged PCR products
    • Eurofins Genomics ligates sequencing adaptors for Illumina sequencing
    • Optionally: Free primer design starting from target specific sequences

     

    INVIEW CRISPR Check (300 - 570 bp)

     

    • You prepare amplicons from your samples with specific universal overhangs
    • Indexing of all samples @ Eurofins Genomics
    • Optionally: Free primer design starting from target specific sequences

     

    More information about both products can be found below

     

     

    >> FAQ's CRISPR Check

    >> NGS Prepaid Coupons

    >> Sample Submission

     

     

     

    Find your optimal INVIEW CRISPR Check solution here

     

     

    INVIEW CRISPR Check (size: 150- 270 bp)

    Highest flexibility in amplicon size and data packages

     

    INVIEW CRISPR Check Adaptor Ligation starts from short PCR samples prepared in your lab that you optionally provide with molecular tags. Eurofins Genomics continues with adaptor ligation on each PCR sample or sample pool and thus introduces sequencing adaptors necessary for Illumina sequencing.

     

    Highlights of INVIEW CRISPR Check Adaptor Ligation

    • Sequencing of (Wildtype) amplicons up to 270 bp
    • Large selection of Illumina sequencing options
    • Flexible data packages, starting from 5M read pairs packages
    • Pooling of up to 192 samples
    • Bioinformatics pipeline specially designed for identification and quantification of CRISPR events
    • Free of charge Eurofins Primer design for application of molecular tags to save your time  and to ensure best practice multiplexing 

     

    You have a large number of samples that you would like to analyse using this workflow? In these cases we can offer a customised Project.

     

    You have applied the HDR repair mechanism? Please contact us for a customised project discussion.

    You Need Oligos to prepare your Amplicons? see below

     

     

     

    Product details

     

    Starting Material

     

    Library Type 

    Starting Material  

     
    Illumina adapter ligation library

    100 - 200 ng PCR product / amplicons (up to 100 µl, concentration > 1 ng/µl

    Amplicon length requirements:

    Sequencing with 5M read pairs package(s), 2 x 150 bp: 

    For assessment of deletions of up to 50 bp and insertions of up to 30 bp, the wildtype amplicon size (not including the tags) should be between 150 and 225 bp.

    Further sample requirements can be found in the sample submission guide. 

     

     

     Table 1. Available libraries and accepted starting material* for INVIEW CRISPR Check Adaptor Ligation.

     

    Specification: 

    • Illumina data packages
      • Guaranteed 5M read pairs (10 million reads) per package, 2 x 150 bp

     

     

    INVIEW CRISPR Check (size: 300 - 570 bp)

    Just let us do the barcoding and pooling work for you

     

    INVIEW CRISPR Check for larger amplicons starts from PCR samples prepared in your lab with universal adaptors. Eurofins Genomics will do the indexing of all samples.

     

    For sample preparation you just need to add a universal sequence adaptor to your target specific primers. For amplification of the same target region in a series of samples, you thus need only one primer pair per target.

     

    Highlights INVIEW CRISPR Check 2nd PCR:

    • No need for your own barcoding - only one primer pair per target region
    • No need for exact quantification of purification of amplicons
    • Cost-effective outsourcing of the remaining steps
    • Bioinformatics pipeline specially designed for identification and quantification of CRISPR events
    • Easy primer design and primer ordering through Eurofins 

     

    You have a large number of samples that you would like to analyse using this workflow? In these cases we can offer a customised project with full MiSeq runs. Please contact us for a project discussion.

     

    You Need Oligos to prepare your Amplicons? See below

     

     

    Product details

     

    Starting material

     

    Library Type 

    Starting Material  

    Illumina 2nd PCR library

    25 µl of purified amplicon, preferred in EB buffer (PCR product)*

    Amplicon size needs to be adapted to the length of InDels you want to be able to analyse and must allow merging of read pairs. E.g. for assessment of deletions of up to 50 bp and insertions of up to 30 bp, the wildtype amplicon size (not including overhangs 1st PCR) should be between 400 and 500 bp.

    Quality check need to be performed by agarose gel electrophoresis (= clear visible band(s) of expected size using 5 µl PCR product). 

    Samples need to be shipped in 1,5 ml safe-lock tubes.

     

     Table 1. Available libraries and starting material*

     

    Specification:

     

    Illumina data packages:

    • Guaranteed 60k read pairs per amplicon
    • 2 x 300 bp read mode

     

    CRISPR Bioinformatics (for both approaches)

    Analysis

     

    • Data sorting according to tags designed by Eurofins Genomics
    • Data sorting according to customer supplied tag
    • CRISPR mutation analysis

     

    The CRISPR mutation analysis evaluates mutations introduced by non-homologous end joining (NHEJ) pathway. Our pipeline is designed for analysing events with single breaks.

     

    • Quality clipping and merging of raw reads
    • Mapping to target region(s) and quality filtering of mapped reads
    • Clustering based identification and quantification of NHEJ events
    • Quantification of mutation rates and of frameshift mutations
    • For each sample and target region, detailed reporting of:
      • Wildtype and alternate allele sequences including quantification
      • Observed mutation rate plots, frameshift classification plots
      • Raw data, mapping, and coverage statistics

     

     

    Deliverables

     

    • Comprehensive report with results and methods sections
    • Raw FASTQ files
    • Quality clipped and assembled FASTQ files
    • Mapped reads in BAM format (raw and filtered)
    • Observed wildtype and alternate allele sequences including quantification
    • Raw data, preprocessing, mapping, and coverage metrics in CSV format

     

    NGS Oligos

    NGSgrade Oligos (amplicon size: 200 - 225 bp)

     

    How to prepare your Amplicons for INVIEW CRISPR Check Adaptor Ligation

     

    It's simpler than ever

    For the adaptor ligation protocol, you can either provide plain amplicons for adaptor ligation or  make use of our tagging strategy. The latter reduces sequencing costs especially for projects involving more than a few samples.

    Option without tagging:

    • Define the loci(s) you want to amplify and design target specific primer sequences that amplify the selected region.
    • Order target specific NGSgrade oligo primers and prepare amplicons
    • Check amplification success on a gel and purify the amplicons
    • As no tagging has been performed a minimum of 1 data package has to be ordered per amplicon

     

    Option with tagging:

    • Define the loci(s) you want to amplify and design target specific primer sequences that amplify the selected region.
    • Upload your target specific sequence(s) on our corresponding oligo order page and let Eurofins do the automatic primer design.
    • Order primers and prepare amplicons with the designed primers.
    • Check amplification success on a gel & purify individual amplicons or your amplicon pool 
    • The use of tagged primers enable multiplexed sequencing of several pooled amplicons within a single data package.

     

     

     

    NGSgrade Oligos (amplicon size: 450 - 500 bp)

     

    How to prepare your Amplicons for INVIEW CRISPR Check 2nd PCR

     

    It's as simple as it can be

    • Define the locus or loci you want to amplify and design target specific primer sequences that amplify the region(s).
    • Upload your target specific sequence on our corresponding oligo order page and let Eurofins do the automatic primer design.
    • Order NGSgrade primers and prepare amplicons with the designed primers.
    • Check amplification success on a gel and send unpurified amplicons to Eurofins.

     

    Do you have more Questions? Please contact us.

     

     

     

    Delivery time

    Adaptor ligation:

     

    Delivery time starting from 20 working days.

     

    2nd PCR:

     

    • 15 - 20 working days for up to 96 samples.
    • 3 additional working days for the bioinformatics analysis

     

    Literature

     

    NGS INVIEW CRISPR Check demo report

    You'll get a demo PDF report, but your comprehensive analysis report will be in interactive HTML format.

     

     

     

     

     

    Solutions you may also like

     

     



    NGSgrade Oligos


    Improve your NGS data


    >> READ MORE

     

     

     

    NGS Coupons


    The perfect prepaid solution for your NGS projects


    >> READ MORE

     

     

     

    EVOCard


    The prepaid card for more
    flexibility in your research


    >> READ MORE

     

     

    Quality is important for us at Eurofins

    Our products and services are produced and performed under strict quality management and quality assurance systems.

    Find certificates here
    Production Site
    • Europe
    • America
    • India
    • Japan
    Eurofins Genomics
    • Terms & Conditions
    • Sitemap
    • Imprint
    • Privacy
    • Licenses
    • Cookie Settings
    Contact
    General Customer Support
    phone +49 7531 816068
    Toll free phone number for
    Europe: 00800 200 100 20
    support-eu@genomics.eurofinseu.com

    Eurofins Genomics Europe Shared Services GmbH
    Anzinger Str. 7a
    85560 Ebersberg
    Germany

    /p>

    2025 - Eurofins Genomics

    VEGA Beta