Single-internal-quenched qPCR probes featuring enhanced quenching properties.- Internal quencher for highest quenching efficiency
- In combination with a proprietary High Resolution HPLC purification method
Specifications:- Sequence length from 10 - 40 bases
- Delivered dried at specified quantity or liquid at defined concentration
- Selectable solvents are bidest water or TE buffer (10 mM Tris, 1 mM EDTA; pH=8)
- An online QC Report including the MALDI-TOF MS traces is available free of charge
- Additional services such as custom normalization or aliquoting are available upon request
How to request a quote:First select the desired input format. The dyes and quencher and further options can be selected in the next step.
Place the internal quencher between the
9th and 10th base from the 5' sequence end.
Example: [FAM]
ATCGATCGA[BHQ1INT]
TCGATCGATCGATCG[PHO].
The 3’-phosphate modification is selected automatically and mandatory. Check your entries and then click on "Add to cart".
Sequence info:ACGT = DNA;
{ACGT} = LNA;
I = 2’-Desoxyinosine; U = 2’-DesoxyuridineUse the
IUB code for wobble bases (equal amount of degenerated bases)
If you need an oligo modification which is not listed in the dropdown, please select "EXTRA [EXT]" and state the required modification in the production comment before you submit your quote request.
Special requests:Additional services can be requested by describing your needs in the production comment. Our experts will create the right offer for you, which can be accepted online.
Please note:Please order probes labeled with Cy3, Cy5 or Cy5.5 dry or dissolved in water, as they are only stable at pH 7. A higher pH would damage the dye and thus the probe. More information can be found in our Product FAQs.
Ultimate Precision Probes are recommended and optimised for qPCR analysis. For genotyping assays we recommend our Dual Labeled Probes.
Please let us know in the production comment section if you intend to use the requested probes directly in commercial kits to ensure that the offer is prepared accordingly.