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    ONT Lite Bacterial Genome Sequencing

    • You are here:
    • Next Generation Sequencing >
    • Applications >
    • Bacterial genome sequencing on ONT
     

     

    Bacterial genome sequencing using Oxford Nanopore Technology.



    Tailored for the complete sequencing and single contig assembly of genomic DNA (gDNA) from an individual, potentially unknown bacterial strain, our bacterial genome sequencing service employs state-of-the-art Oxford Nanopore Technologies (ONT) long-read sequencing technology.

     

    Take advantage of our super low prices and exceptionally fast turnaround time.

     

     

    >> Order now

    Sample Barcodes

    prepaid coupons

    >> Hybrid assembly

     

    Specification

     

    • Sequencing on Oxford Nanopore GridION
    • On average 30x genome coverage
    • De novo assembly and QC, species identification, and annotation included in the package
    • Prepaid option available
    • From one sample
    • Great price, fast turnaround time
    • For all bacterial genomes up to 7 Mb

     

    Applications

     

    • De novo assembly of bacteria (< 7 Mb) and intrinsic plasmids (no reference sequence required)
    • Sequencing of a clonal population (individual strain, potentially unknown)
    • Species determination
    • Sample clonal/purity check after the assembly

     

    Need more?

     

    • Your bacterial genomes are larger?
    • You need more coverage?
    • You need a hybrid assembly?

     

    Learn more on our "Complete ONT portfolio overview">>

     

     

    >> FAQ's Bact. wGS

    >> Trouble- shooting

    >> how to redeem prepaid coupons

    >> Sequencing Accessories

     

     

     

     

    Product Specifications & Ordering

     

     

    Product details / workflow

    • No sample number limitation, dropbox shipment possible 
    • Super fast turn-around time in 5-7 working days
    • Prepaid option available
    • On average 30x genome coverage
    • De novo assembly and QC, species identification, and annotation included in the package
    • Library preparation (amplification-free, including minimal fragmentation); using v14 library prep chemistry
    • Sequencing using Oxford Nanopore GridION (flow cell R10.4.1)

     

    Data delivery & Bioinformatics deliverables

     

    The following files will be delivered for each bacterial genome sample

     

    • Comprehensive data analysis report with assembly statistics and QC in HTML format
    • FASTQ raw data delivered which align to the consensus sequence
    • Assembled genome sequence in FASTA format
    • Circular genome plot as a publication-ready PNG
    • Annotated genome sequence of all contigs in GENBANK format (import directly into your favourite software like Sanger's Artemis, SnapGene, Geneious Prime, Benchling, SeqBuilder Pro etc.)
    • Annotated genome features in GFF3 format
    • Annotated feature table, including the nucleotide sequence of each feature
    • Nucleotide sequences of all gene sequences in FASTA format
    • Amino acid sequences of all annotated protein sequences in FASTA format

     

    Your data will be securely delivered through your Ecom account

     

    No delivery of: Pod5 or FASTQ files that don't align.

    If you are interested in Pod5 files and not-aligned FASTQ files please contact us, regarding our premium service.

    Delivery time

    The turnaround time for bacterial whole genome sequencing is on average 5 - 7 business days.

    You will be informed via Email, when your results are available in your Ecom account.

    Sample Preparation & Submission


    Use the following concentration and volume for your samples:

    Sample type
    Length
    Concentration Min. Volume
    Bacterial genome up to 7 Mb 30 ng/µl 20 µl

    Please note: Samples that do not meet our concentration requirement might fail in sequencing. No repetition will be performed on failed samples due to wrong conc adjustment.

     

     

    For DNA sample preparation it is sufficient to use a high quality column based DNA preparation kit. No high molecular weight DNA is required.

     

     
    Please send your sample tubes according to the requirements below:

    • Use 1.5 ml safe-lock tubes for your samples
    • Please provide your samples either in nuclease-free water or elution buffer (10 mM Tris, pH 8.5)
    • Please do not use Nanodrop for your concentration measurement. Spectrophotometric techniques are unreliable for the quantification measurement. Please use a fluormetric method, like Qubit.
    • Do not tape or wrap tubes with parafilm. Safe-lock tubes offer perfect sealing and evaporation protection
    • Label your sample tubes with our free barcode labels. Affix the label horizontally

     

    How and where to ship your samples:

    • Use either one of your nearest DropBox for free sample pick up or a courier of your choice
    • You can convinently use one of our sample shipment options to send your samples
    • To send your samples to us safely, make use of our sample bags and boxes
    • Send your samples to our Eurofins Genomics sequencing lab in Cologne, Germany

     

    Please ensure that your sample is free of any contaminants (e.g., ethanol) that could negatively affect your results. A complete list of contaminants can be found here: https://community.nanoporetech.com/contaminants 

     

     

     

     

    Literature

     

    Demo report for bact. WGS

    You'll get a demo PDF report, but your comprehensive analysis report will be in interactive HTML format.

    Certified service provider for ONT

    Sample submission guide PDF

     

     

     

     

     

     

     

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