Whole Genome sequencing with long read sequencing. Suitable for large fragment DNA samples. Novel Nanopore technology is used to obtain very long sequences of several kilobases.
For all prokaryotic samples.
If you are interested in whole genome sequencing of eukaryotes using ONT please contact us >>
Applications
- Resequencing of whole genomes
- Assembly of genomes
- Identification of taxonomic background
- Metagenomic analysis of long reads
Highlights
- Long read recovery of several kilobases
- Flexible data output for your sample
- Available from one sample
- Polishing (hybrid assembly) with Illumina data possible
Product description:
- Sequencing:
- Technology: Oxford Nanopore
- Output read length: Equal to fragment length (can be up to several kbp)
- Throughput is dependent upon the quality of sample submitted
- Data output: Approx. 7 Gb per Flow Cells (dependent on DNA quality and length)
- No Output guarantee per Flow Cell or sample!
- Library preparation:
- Multiplexing samples possible by using the ONT native barcodes
- Deliverables:
- Raw Nanopore sequencing data (FastQ)
- De novo assembled contigs (FASTA)
- Additional bioinformatics analysis available
| No of samples / Flow Cell |
Single |
2 - 4 |
> 4 |
| Minimum amount / sample |
2000 ng |
1000 ng |
400 ng |
| Recommended amount* / sample |
4000 ng |
2000 ng |
800 ng |
| Minimum concentration |
40 ng/µl (fluorometer, e.g. Qubit)
100 ng/µl (spectrophotometer, e.g. Nanodrop)
|
| Minimum volume / sample |
50 µl |
25 µl |
15 µl |
*Customers should be aware that loading the optimal amount of library will maximize the yield of generated sequencing data for their samples. Loading less or DNA of low quality will have a detrimental effect on Flow Cell health and thus output.
To avoid sending samples with potential contaminations please check the absorption ratios of the sample with spectrophotometer, e.g. Nanodrop:
OD 260/280: ~1.8
OD 260/230: 2.0-2.2
The read length distribution is highly dependent on the fragment size of the provided genomic DNA. Please ensure that gDNA has long fragments with a limited number of short fragments. We recommend to check your DNA fragment size on a Gel or Tapestation/Fragment analyser .
Microbiome or community analysis with16S rRNA full length sequencing with Oxford Nanopore sequencing technology. Higher taxonomic resolution through availability of the whole 16S rRNA gene.
Applications
- Characterization of strains or communities
- Identification of taxonomic background
- Metagenomic analysis of long reads
More details can be found here >>
Sequencing of large and/or repetitive amplicons, amplicon mixtures, and amplicons of any size.
Product description:
- Library preparation:
- ONT adapter ligation (multiplexing depends on amplicon size and preferred coverage, max. 96 samples per Flow Cell)
- Sequencing:
- Technology: Oxford Nanopore
- Output read length: Equal to fragment length (can be up to several kbp)
- Throughput is dependent upon the quality of sample submitted
- Approx. 7 Gb per Flow Cells (dependent on DNA quality and length)
- Deliverables:
- Raw Nanopore Amplicon data (FastQ)
- BioIT on request (e.g. Variant analysis VCF-file)
- Input required (dependent on multiplexing):
- OD 260/280: 1.8oOD 260/230: 2.0-2.2
- Max. amplicon size: 20 kb
- For 1 sample:
- Optimal: >25ng/μl in 60 μl
- For 2-96 samples:
- Size range of different amplicons should be similar (+/-10%)
- Optimal: >25ng/μl in 30 μl
Additional bioinformatics option:
ONT Amplicon Bioinformatics Analysis
- Sequence Quality Assessment
- Amplicon consensus generation
- Amplicon variant sites
- Reporting & Consultancy
Deliverables
- Raw Nanopore Amplicon data (FastQ)
- Raw Nanopore sequencing data (pod5) available on request (additional costs)
- BioIT on request (e.g. Variant analysis VCF-file)
Confirm your full plasmids faster, more accurately, and more affordably than ever before. NGS generation 3 technology has made it possible to quickly sequence whole plasmids in a fraction of the time and without the hassle of having to design and synthesize primers.
Applications
Whole plasmid sequencing using NGS Gen 3 technology is a powerful and accurate method for characterizing and analyzing plasmids, which makes it well suited for a wide range of applications.
- Plasmid Verification
- Resequencing of whole genomes
- Assembly of genomes
- Identification of taxonomic background
- Metagenomic analysis of long reads
- Much more
You can order this product here >>
Specifications
- Amplicon size: 1 kb - 25 kb
- Short turnaround time
- From one sample
- Dropbox shipment possible
- Prepaid option available
- Bioinformatics analysis included
- Great price
All details about this product can be found here >>
Specifications
- Sequencing on Oxford Nanopore GridION
- On average 30x genome coverage
- De novo assembly and QC, species identification, and annotation included in the package
- Prepaid option available
- From one sample
- Great price, fast turnaround time
- For all bacterial genomes up to 7 Mb
All details about this product can be found here>>