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Synthetische Gene & GeneStrands

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  • Gensynthese

 

 

Der Universalschlüssel zur Molekularbiologie.

 

 

 

 

GenSynthese

GeneStrands

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>> GenEIUS

 

 

 

Anwendungsmöglichkeiten

 

Verbesserung der Expression in heterologen Systemen durch

  • “Codon Usage”-Anpassung
  • Die Verwendung von Promoter- und Enhancer-Elementen

 

Klonierungsanwendungen

  • Subklonierung
  • cDNA-Klonierung
  • Einsatz als qPCR und PCR-Standards

 

Optimierung von Konstrukten

  • Einbau von gewünschten bzw. Entfernung von unerwünschten Motiven
  • Herstellung von Hybridgenen
  • Screening nach Proteinen mit verbesserten Eigenschaften

Unschlagbare Preise - ab nur €59

 

  • GeneStrands - dsDNA Fragmente für nur €59
  • Erhalten Sie Ihr kloniertes Gen für nur €99
  • Komplette Genbibliotheken gibt es bei uns schon ab €309

 

 

 

 

>> FAQ's Gene Synthese

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Produkt-Übersicht & Ausgewählte Publikationen

 

 

Wählen Sie Ihr passendes Produkt

Je nach Länge und Komplexität eines Gens oder Projekts können Sie verschiedene Kategorien synthetischer Gene wählen.

 

Standard-Gene

Standard-Gene werden nach den folgenden Kriterien definiert:

  • Moderater GC-Gehalt (36 - 74 %)
  • Keine ausgeprägten Repeats (> 17 bp), 2-Mismatch Repeats (> 27 bp) oder kritischen Hairpin-Strukturen
  • Keine ausgeprägten Homopolymer-Abschnitte (> 8 bp)
  • Gesamt-GC-Gehalt in 100 bp Abschnitten unter 74%

 

Komplexe Gene

Wenn ein oder mehrere der unten aufgeführten Kriterien für Ihr Projekt zutreffen, dann handelt es sich laut unserer Definition um ein komplexes Gen:

  • Extremer GC-Gehalt (sehr hoch oder niedrig)
  • Lange Repeats oder Sekundärstrukturen im Gen
  • Sie wünschen begleitende Beratung bei Ihrem Projekt

 

GeneStrands

GeneStrands ist eine kostengünstige Alternative für kurze Gene ohne komplexe Strukturen:

  • Lieferzeit: ab 5 Arbeitstagen
  • GeneStrands Längen: 20 - 2000 bp
  • GeneStrands werden als lineare dsDNA-Fragmente ausgeliefert

 

Genbibliotheken

Für ihre Screening Projekte zur Verbesserung der Proteineigenschaften empfehlen wir Ihnen unsere Genbibliotheken. Unser Angebot für Sie umfasst:

  • Mutationen an einzelnen Positionen
  • Mutationen an mehreren Positionen
  • Mutationen in spezifischen Domänen
  • Zufällige Substitutionen

Ausgewählte Publikationen zum Thema Gensynthese

2021

SARS-CoV-2: Anna et. al., High seroprevalence but short‐lived immune response to SARS‐CoV‐2 infection in Paris

Gensynthese: Marusic, et al., The expression in plants of an engineered VP2 protein of Infectious Bursal Disease Virus induces formation of structurally heterogeneous particles that protect from a very virulent viral strain

Gensynthese: Wang, et al., A glycoengineered antigen exploiting a conserved protein O-glycosylation pathway in the Burkholderia genus for detection of glanders infections

2020

Gensynthese: Wong T.S., Tee K.L. (2020) Gene Cloning. In: A Practical Guide to Protein Engineering. Learning Materials in Biosciences. Springer, Cham

SARS-CoV-2: Ruiz-Arrondo., et al., Detection of SARS‐CoV‐2 in pets living with COVID‐19 owners diagnosed during the COVID‐19 lockdown in Spain: A case of an asymptomatic cat with SARS‐CoV‐2 in Europe

Gensynthese: Ferrari et al., The allergen Mus m 1.0102: Cysteine residues and molecular allergology, Molecular Immunology

Gensynthese: Fernandes et al., The dimerisable Cre recombinase allows conditional genome editing in the mosquito stages of Plasmodium berghei

2019

Gensynthese: Abu-Halima M., et al., Differential expression of miR-23a/b-3p and its target genes in male patients with subfertility

Gensynthese: Johari Y. B., et al., CHO genome mining for synthetic promotor design, 

Gensynthese: Sota M., et al., Efficient transposition of Tn4556 by alterations in inverted repeats using a delivery vector carrying a counter-selectable marker for Streptomyces

Gensynthese: Jimmy S., et al., Discovery of small alarmone synthetases and their inhibitors as toxin-antitoxin loci

Gensynthese: Liu J., et al., Polychromatic solar energy conversion in pigment-protein chimeras that unite the two kingdoms of (bacterio)chlorophyll-based photosynthesis

Gensynthese: Tebo AG., et al., A plit fluorescent reporter with rapid and reversible complementation

 

2018

Gensynthese: Jaubert, C. et al., RNA synthesis is modulated by G-quadruplex formation in Hepatitis C virus negative RNA strand

Gensynthese: Fusco, F. A. et al., Biochemical charaterization of a thermostable endomannanase / endoglucanase from Dictyoglomus turgidum

Gensynthese: Bernardini A. et al., The phosphorylatable Ser320 of NF-YA is involved in DNA binding of the NF-Y trimer

Gensynthese: Amiri, M. M. et al., Hersintuzumab: A novel humanized anti-HER2 monoclonal antibody induces potent tumor growth inhibition

Genesynthese: Fathi, S. et al., Design and construction of a new recombinant fusion protein (2b2t+EPC1) and its assessment for serodiagnosis of cystic echinococcosis

Gensynthese: Brenner, N. et al., Validation of Multiplex Serology detecting human herpesviruses 1-5

Gensynthese: Nolvak, H. et al., Reduction of antibiotic resistome and integron-integrase genes in laboratory scale photobioreactors treating municipal wastewater

Gensynthese: Chow J., et al., The Thaumarchaeon N. gargensis carries functional bioABD genes and has a promiscuous E. coli deltabioH-complementing esterase EstN1

 

2017

Gensynthese: Le, T, M. et al., Vectors for Expression of Signal Peptide-Dependent Proteins in Baculovirus/Insect Cell Systems and Their Application to Expression and Purification of the High-Affinity Immunoglobulin Gamma Fc Receptor I in Complex with Its Gamma Chain.

Gensynthese: Mekonnen S. et al., O-5S quantitative real-time PCR: a new diagnostic tool for laboratory confirmation of human onchocerciasis

Gensynthese: Wingen, M. et al., Novel Thermostable Flavin‐binding Fluorescent Proteins from Thermophilic Organisms

 

2016

Gensynthese: Nolvak H., et al., Inorganic and organic fertilizers impact the abundance and proportion of antibiotic resistance and integron-integrase genes in agricultural grassland soil

Gensynthese: Whitten MM., et al., Symbiont-mediated RNA interference in insects

Gensynthese: Ohno M., et al., Acidic mammalian chitinase is a proteases-resistant glycosidase in mouse digestive system

Gensynthese: Hoshiba T., Decellularized matrices as in vitro models of extracellular matrix in tumor tissues at different malignant levels: Mechanism of 5-fluorouracil resistance in colorectal tumor cells

Gensynthese: Turner K.B., et al., Next-Generation Sequencing of a Single Domain Antibody Repertoire Reveals Quality of Phage Display Selected Candidates

Gensynthese: Sudo Y., et al., Functional and Photochemical Characterization of a Light‐Driven Proton Pump from the Gammaproteobacterium Pantoea vagans

Gensynthese: Scorsone E., et al., Major Urinary Proteins on Nanodiamond-Based Resonators Toward Artificial Olfaction

2015

Gensynthese: Brieke, C. et al., Rapid access to glycopeptide antibiotic precursor peptides coupled with cytochrome P450-mediated catalysis: towards a biomimetic synthesis of glycopeptide antibiotics. Organic & biomolecular chemistry

Gensynthese: Takeishi, R. et al., Epimerization at C-3′′ in Butirosin Biosynthesis by an NAD+-Dependent Dehydrogenase BtrE and an NADPH-Dependent Reductase BtrF. ChemBioChem

 

2014

Gensynthese: Buettner, K. et al., Protein surface charge of trypsinogen changes its activation pattern. BMC biotechnology 14(1)

Gensynthese: Chiwata, R. et al., None of the Rotor Residues of F 1-ATPase Are Essential for Torque Generation. Biophysical journal 106(10)

Gensynthese: Holmberg, M. et al., A versatile bacterial expression vector designed for single-step cloning of multiple DNA fragments using homologous recombination. Protein expression and purification 98

Gensynthese: Dietz O. et al., Characterization of the small exported Plasmodium falciparum membrane protein SEMP1. PLOS ONE 9(7): e103272

Gensynthese: Canali E. et al., A high-performance thioredoxin-based scaffold for peptide immunogen construction: proof-of-concept testing with a human papillomavirus epitope. Nature Scientific Reports 4(4729)

2013

Gensynthese: Mueller W. et al., Hierarchical organization of multi-site phosphorylation at the CXCR4 C Terminus, PLOS ONE 8(5): e64975

Gensynthese:
 Van Noort, V., Bradatsch, B., Arumugam, M., Amlacher, S et al., Consistent mutational paths predict eukaryotic thermostability. BMC Evolutionary Biology, 13(1), 7

2012

Gensynthese: Carciofi M. et al. Concerted suppression of all starch branching enzyme genes in barely produces amylose-only starch granules. BMC Plant Biology 2012, 12:223

Gensynthese: Lejonklou M. et al. Accelerated Proliferation and Differential Global Gene Expression in Pancreatic Islets of Five-Week-Old Heterozygous Men1 Mice: Men1 Is a Haploinsufficient Suppressor. Endocrinology April 2012 (Genes purchase/synthesis: kinase complex-associated protein (Ikbkap)

Gensynthese: Ritari J. et al. Detection of Human Papillomaviruses by Polymerase Chain Reaction and Ligation Reaction on Universal Microarray. PLoS ONE 7(3): e34211. (HPV sequences were synthesised and cloned into plasmid vector)


2011

Gensynthese: Weber E. et al. Assembly of Designer TAL Effectors by Golden Gate Cloning. PLoS ONE 6(5): e19722

Gensynthese: Gays F. et al. Comprehensive Analysis of Transcript Start Sites in Ly49 Genes Reveals an Unexpected Relationship with Gene Function and a Lack Of Upstream Promoters. PLoS ONE 6(3): e18475.

 

 

Literatur

 

Application Note: GENEius and competitor algorithms

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