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Eingangsmenge / Konzentrationsbereich / RIN |
Definition der Platten |
Weitere Anmerkungen |
| RNA (Poly A selection) |
150 ng,
6-200 ng/µL,
RIN ≥ 8 |
semi-skirted*
bitte Position H12 leer lassen |
min. 25 µl |
| RNA (depletion) for bacteria rRNA, HMR rRNA and globin |
375 ng,
37,5-200 ng/µL,
RIN ≥ 8 |
semi-skirted*
bitte Position H12 leer lassen |
min. 10 µl |
| rRNA depleted / mRNA angereichert durch den Kunden |
min 20 ng,
> 1.4 ng/µL,
RIN ≥ 8 |
semi-skirted*
bitte Position H12 leer lassen |
bis zu 50 µl |
| RNA FFPE |
200 ng,
8-200 ng/µl,
DV200 > 35% |
semi-skirted*
bitte Position H12 leer lassen |
min. 25 µl |
| RNA ultra low |
2 ng, ≥ 15 µL with
0.45-10 ng/µL,
RIN > 8 |
semi-skirted*
bitte Position H12 leer lassen |
min. 15 µl |
| RNA Exome |
FFPE: 150 ng, 18-200 ng/µl, DV200 > 35%
Intact RNA: 20 ng, 2,5-200 ng/µl, RIN > 8
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semi-skirted*
bitte Position H12 leer lassen |
min. 15 µl |
| TruSeq RNA |
300 ng,
6-500 ng/µl,
RIN > 8 |
semi-skirted*
bitte Position H12 leer lassen |
min. 50 µl |
| miRNA (QiaSeq) |
150 ng,
30-200 ng/µl,
RIN > 8 |
semi-skirted*
bitte Position H12 leer lassen |
min. 10 µl |
| DNA |
100 ng /
0.5-25 ng/µl |
semi-skirted* |
min. 25 µl |
| Enrichment |
100 ng /
1-50 ng/µl |
full-skirted* |
min. 25 µl |
| Enrichment FFPE |
200 ng /
2-50 ng/µl |
full-skirted* |
min. 25 µl |
| Amplicon /Microbiome |
200 ng /
10-50 ng/µl |
semi-skirted* oder Deepwell Plate* |
min. 25 µl |
| Microbiome 2nd PCR |
10 ng /
0.5 ng/µl |
full-skirted*
bitte Positionen G12 & H12 leer lassen |
min. 25 µl |
| Adapter Ligation |
100 ng-200 ng |
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| Enrichment cfDNA |
10 ng-100ng |
full-skirted* |
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| RNA for RNA fusion detection |
FFPE: 400 ng, min. 40 - 80 ng/µl
Intakte RNA (fresh frozen): 100 ng, min. 20 ng/µl
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