Great flexibility based on your requirements
SUPREMERUN 48 & 96 - Cost-efficient sequencing
SUPREMERUN 48 and SUPREMERUN 96 is a high-throughput sequencing service for routine Sanger sequencing of 48 or 96 samples on a plate. Leading technologies and automated workflows guarantee easy and convenient sample processing. SUPREMERUN 96 enables transparent sample tracking during the automated sequencing workflows with prepaid SupremeRun 96 barcodes.
- Sanger sequencing of plasmids or PCR fragments in plates
- Cost-effective sequencing of large number of samples
- Up to 1,000 nucleotides in Phred20 quality
- Easy online order process with simple invoicing
Order your SupremeRun 96 Barcodes now!
SUPREMERUN | Multiprimer - Exceptional versatility
SUPREMERUN | Multiprimer is a sophisticated Sanger sequencing service that offers maximum flexibility for sequencing in 96well plates. This product is ideal for high-throughput projects that require sequencing of many PCR products or plasmid DNA samples sequenced with different primers.
- Sanger sequencing of plasmids or PCR fragments in plates
- Combine up to four individual primers for one template (DNA)
- Flexible well assignment
Product details of the SupremeRun Plate services
- High-throughput Sanger sequencing of up to 96 samples on a microtiter plate
- Full 96well microtiter plate sequencing for 96 or 48 samples with one primer
- Up to 384 combinations possible with up to four sequencing primers (Multiprimer)
- Free use of a wide variety of universal primers
- Sequencing reads automatically processed with superior data analysis software
- Reads with up to 1,100 nt in Phred20 quality
- Optimised Sanger sequencing protocols for difficult templates (GC-rich sequences, bisulfit samples or low copy plasmids)
- Free storage of DNA and primers
- Repetition of sequencing reactions upon request (free of charge)
Storage of DNA and primer:
- Storage of DNA and primers delivered by customer: 10 days
- Custom primers synthesized will be stored for 6 months
- For a new order after these 6 months of storage and within the following 6 months, a new primer aliquot will be ordered free of charge
Data delivery in common file formats to your secure account
- Chromatogram (.ab1, ABI file)
- Text file (.seq, SEQ file)
- Fasta format (.fas, FAS file)
Data are stored and available online for 6 months.
Sample & Primer requirements
Concentration:
- Plasmids: 30-100 ng/µL
- PCR fragments: 10-50 ng/µL
- Custom primers: 10 pmol/µL
Sample Volume:
DNA samples: 20 µL for each well
Please send the DNA dissolved in water. The solution must not contain any EDTA. DNA to be delivered in 96 well PCR plates with V-shaped wells.
Primer Volume:
For SupremeRun 48 & 96:
50 µl (sufficient for one plate) delivered in 1.5 ml tubes
For SupremeRun| Multiprimer:
100 µl (sufficient for up to 50 reactions) delivered in 1.5 ml tubes
Please do not use parafilm to seal. Please close with cap strips.
Primer characteristics:
The melting temperature (TM) of the primer should be between 52°and 58°C and the length should be between 17-19bp. Ideally, the GC content should be 10 G+C for a 17mer; 8-9G+C for an 18mer and 7-9 G+C for a 19mer.
G or C should be at the 3’ end, but not more than 3 Gs or Cs. The primer sequence should be a good mix of all 4 nucleotides with no more than 4 identical bases in a row (AAAA or GGGG).