Supreme sequencing in 96well plates
SupremeRun 96 - Cost-efficient high throughput sequencing
SupremeRun 96 is a high-throughput sequencing service for routine Sanger sequencing of 96 samples with the same primer on a plate.
Automated workflows guarantee easy and convenient sample processing. Prepaid SupremeRun 96 barcodes enables transparent sample tracking during the automated sequencing workflows.
- Sanger sequencing of plasmids or PCR fragments in plates
- Reading lengths of up to 1100 Q20 bases
- Easy online ordering with simple invoicing
SupremeRun Multiprimer - Exceptional versatility
SupremeRun Multiprimer offers maximum flexibility for sequencing in 96well plates. This product is ideal for high-throughput projects that require sequencing of many PCR products or plasmid DNA sequenced with different primers.
- Sanger sequencing of plasmids or PCR fragments in plates
- Combine up to four individual primers for one template (DNA)
- Flexible well assignment
Please Note:
The SupremeRun 48 sequencing service has been discontinued. We still accept 48 samples in 96well plates and you can submit these using our SupremeRun Multiprimer service or our PlateSeq Service.
Product details of the SupremeRun Plate services
- High-throughput Sanger sequencing of up to 96 samples on a microtiter plate
- Full 96well microtiter plate sequencing for 96 samples with one primer
- Up to 384 combinations possible with up to four sequencing primers (Multiprimer)
- Free use of a wide variety of standard primers
- Sequencing reads automatically processed with superior data analysis software
- Reads with up to 1100 Q20 bases
- Optimised Sanger sequencing protocols for difficult templates (GC-rich sequences, bisulfit samples or low copy plasmids)
- Free storage of DNA and primers
- Repetition of sequencing reactions upon request (free of charge)
Storage of DNA and primer:
- Storage of DNA and primers delivered by customer: 10 days
- Custom primers synthesized will be stored for 6 months
- For a new order after these 6 months of storage and within the following 6 months, a new primer aliquot will be ordered free of charge
Data delivery in common file formats to your secure account
- Chromatogram (.ab1 & .scf)
- Text file (.seq clipped & unclipped)
- Fasta files (.fas)
Data are stored and available online for 6 months.
Sample & Primer requirements
Concentration:
- Plasmids: 30-100 ng/µL
- PCR fragments: 10-50 ng/µL
- Custom primers: 10 pmol/µL
Sample Volume:
DNA samples: 20 µL for each well
Please send the DNA dissolved in water. The solution must not contain any EDTA. DNA to be delivered in 96 well PCR plates with V-shaped wells.
Primer Volume:
For SupremeRun 96:
50 µl (sufficient for one plate) delivered in 1.5 ml tubes
For SupremeRun Multiprimer:
100 µl (sufficient for up to 50 reactions) delivered in 1.5 ml tubes
Please do not use parafilm to seal. Please close with cap strips.
Primer characteristics:
The melting temperature (TM) of the primer should be between 52°and 58°C and the length should be between 17-19bp. Ideally, the GC content should be 10 G+C for a 17mer; 8-9G+C for an 18mer and 7-9 G+C for a 19mer.
G or C should be at the 3’ end, but not more than 3 Gs or Cs. The primer sequence should be a good mix of all 4 nucleotides with no more than 4 identical bases in a row (AAAA or GGGG).