Start your CRISPR experiment now
Make use of high quality ready-to-use RNA for your CRISPR experiment.
sgRNA (single guide RNA)
Customer notification: Please note that we discontinued offering sgRNA from March 2020 onwards. You can order this product directly from Synthego. Please contact us in case of any questions.
Our synthetic sgRNA is the easiest way to start your CRISPR experiment, since it is ready for transfection. It has a high editing efficiency and is 100% DNA-free, so there is no risk to integrate foreign DNA into your cell line. It contains a user-defined 17-23mer RNA target sequence and an optimized 80mer Scaffold.
Example format: 5′-AAUUUCACAGCUGCACAUA+ 80mer Scaffold-3'
- Optimised for S.pyogenes
- Includes TE buffer and Nuclease free water.
- 3 nmol (by OD260), sufficient for 10 - 20 transfections
Customer notification: Synthego stopped the production of cr:tracrRNA.
Working with Prokaryotic, Stem Cells and Challenging Cell Lines?
For particular cell types (e.g. primary cells, stem cells, cancer cell lines, prokaryotic cells, plant cells), we recommend to order chemically modified synthetic sgRNAs to increase the stability and therefore the editing efficiency of your experiment.
We offer 2’-O-methyl analogues and 3’ phosphorothioate internucleotide linkages added to the first three 5’ and 3’ terminal RNA residues of your RNA as chemical modifications.
Please note: synthetic RNAs are for Research Use Only
Eurofins is offering synthetic RNAs in cooperation with Synthego.
Need help in designing your CRISPR target sequence?
Use one of the most state-of-the-art design tools