NGS for Agrigenomics

Overview

NGSelect DNA offers easy access to Illumina sequencing technologies in combination with pre-defined modules for pre-sequencing, genomic library preparation and BioIT analysis. The modular set-up shortens delivery times and lowers associated costs. Customers with their own in-house sequencing facility can easily outsource the sequencing workload at peak times for faster data generation with topmost quality. Standardised processes and use of most appropriate methods ensure reliable results and optimal performance.

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Overview

• DNA isolation from various starting material including blood, stool, tissue, cell lines, some bacteria
• Illumina sequencing with 2x150 bp run mode
• Guaranteed coverage for your samples
  • 100 x for bacteria
  • 70 x for yeast
  • 30 x for all larger genomes (up to 180 Mb)
• Bioinformatics analysis
  • Variant analysis: detection and annotation of single nucleotide polymorphism (SNPs) and insertions and deletions (InDels)
  • De novo genome assembly (only for haploid organisms)
• Available from one sample

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Overview

• Two available options - guaranteed reads or lower price
• Very attractive turnaround times starting from 10 working day
• Standard genomic library using UDI’s (unique dual indexing). This is the most reliable indexing standard available and ensures high quality data.
• Highly sophisticated bioinformatics analysis options
• DNA isolation available
• 24/7 online ordering

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Overview

• Taxonomic profiling of bacteria, archaea and viruses with reduced bias due to independence from taxonomic gene markers such as 16S rRNA
• Simultaneous next-generation sequencing of the genomes of all microorganisms in a given sample
• Next-generation sequencing of whole genomes, rather than only specific loci like 16S rRNA
  

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Overview

• Access to cDNA sequencing capacity on the leading Illumina platform
• Choice of different RNA-seq library types, sequencing read modes
• Applicable to any organism from prokaryotes to eukaryotes
• Individual combinations of different parts of the sequencing workflow: cDNA sequencing, cDNA library preparation, pre-sequencing, including RNA isolation and rRNA depletion and BioIT transcriptome analysis

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Overview

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Overview

• Generation of 1 – 4 amplicons per sample (choose from our target list) using a two-step PCR protocol
• Pooling & normalisation of amplicons
• Sequencing on MiSeq with the 2 x 300 bp paired-end read module
• Delivery of guaranteed 60.000 cluster (read pairs) per amplicon
• Sorting of reads according indices & primer sequence clipping
• Merging of overlapping paired reads
• Data delivery via FTP server upload
• Minimum sample number: 1

In addition: high-throughput option available in plate format (starting from 188 samples)

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Overview

NGSelect RTL offers extraordinary fast and reliable sequencing of ready-to-load libraries. Benefit from extensive expertise in sample handling, proprietary protocols, as well as professional Bio-IT analysis.

• Wide range of sequencing options: choose from different reads modes, read numbers, and delivery time options
• Extensive expertise in sample handling: semi-automated processes and optimised use of Illumina flow cells
• Sequencing workflows with ISO17025 accreditation
• Sorting by Illumina Index read at no additional costs
• Accurate determination of library concentration and appropriate sequencing dilution

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NGSelect Amplicons using 2nd PCR approach

NGSelect Amplicon 2nd PCR starts from PCR samples prepared in your lab with universal adaptors (TruSeq adaptors). Eurofins Genomics performs a 2nd PCR step, the index PCR. This PCR step introduces indexed sequencing adaptors nescessary for sample discrimination for pooled sequencing.

For more details see here.

NGSelect Amplicons using Adaptor ligation

NGSSelect Amplicons using Adaptor ligation start from the PCR products prepared in your lab. With Illumina amplicon sequencing, extremely high coverage suitable for discovery of somatic mutations in complex DNA samples can be achieved. Proprietary protocols and years of NGS expertise help provide high quality data with extraordinary fast delivery times.

For more details see here

Amplicon sequencing using Oxford Nanopore 

To learn more about long amplicon sequencing please visit our ONT information page >>