Oligonucleotides are short strands of DNA or RNA molecules that are widely in research, forensics, and genetic testing. The use of the word oligo has widely replaced the use of 'DNA oligonucleotide'. The term oligonucleotide is derived from the Greek word 'oligo', which means few, and nucleotide, which indicates the building blocks of nucleic acids. These oligos, which can have custom sequences, are chemically manufactured and sold to researchers. While typically oligo is used as a prefix to indicate a few, the limits in case of oligonucleotides have not been precisely defined. Eurofins Genomics has chemically synthesize oligomers of lengths ranging from 5 to 200 nucleotides (nt).

Wide range of scales, purification, and options for any project

We perform MALDI-TOF MS analysis on every custom DNA Oligo; infact we pioneered 100% MS testing in 1998. You can place an order for one or several oligos. For orders with large number of oligos, we recommend that you have the oligos delivered in plates.

Production process

The integration of the ecommerce site with the back-end laboratory information managment systems (LIMS) ensures that each synthesized oligo is tracked through the various steps in the production process. As soon as an oligo order is placed on the ecom site, the automated order confirmation email is sent and the details of the order is transferred to the production lab without any loss in the order data. An unique barcode assigned to each oligo order ensures that the production status of an oligo can be monitored at any given instance.

Custom DNA oligos at Eurofins Genomics are synthesized by coupling the relevant series of β-cyanoethyl phorphoramidites on pre-packed controlled-pore solid supports before being deprotected, de-salted, quantitated and being subject to quality control assessment. Typical coupling efficiencies acheived using our automated synthesis platforms are grater than 99%. The coupling efficiencies, when coupled with the length of the oligo sequence to be synthesized, essentially determine the yield of the oligo. Eurofins Genomics has optimized its synthesis scale and sequence length using its traditional high-throughput synthesis platforms to produce high-quality oligos of different lengths.

*All turnaround time estimates are measured in business days

 Express Oligos

Saltfree, Unmodified Oligonucleotides

Purified, Unmodified Oligonucleotides

Purification options available for oligos in tubes and plates based on the length of the oligo and the synthesis scale:

Please note that some oligos, particularly those containing modifications, are offered only with specific purification options. To see the yield of standard unmodified oligos based on the purification and length of the oligo, please visit the yields and purifications page.