w10.0.13 | c2.0.0.5
PROD | u7.5.14
  Quick Order  0
 

Quick Order

X

EVOcard

Oligonucleotide Synthesis

Gene Synthesis & Molecular Biology

Sanger Sequencing

Next Generation Sequencing

Genotyping & Gene Expression

Header Image
 

 

SeqPrimer - The perfect Sanger sequencing primer

 

 

Optimised and validated to detect single base variants in DNA sequences

 


With our validated SeqPrimer, you can be sure to have the optimum primer quality for best sequencing results.

 

 

 

 

 

 

Highlights of our SeqPrimer:


Reproducible high sequence quality:

  • Accurate base detection & sequence interpretation
  • Zero background sequence
  • Sequences with highest possible trace score

 
Optimised & verified SeqPrimer criteria:

  • Average yields of 25 nmol (5 OD)
  • Primer lengths: 15-35 bases (non-defined ratio wobbles possible)
  • SeqPrimer purities of 90%
  • Unique quality criteria ensured by MALDI-TOF MS

 



Flexible delivery formats and fast TAT:

  • Dried down or liquid at selected concentration
  • Production in tube format in 1 working day
  • Production of up to 5 plates in 3-4 working days


Additional online features free of charge:

 

 

 

 

 

 Related Information

 

Technical Information

SeqPrimer - Validated to detect single base variants in DNA sequences


Just because primers work in PCR does not mean they provide good sequencing results.

Standard end-point PCR is an exponential process. The primer quality is not crucial for amplification, when checking PCR products on an agarose gel.

In contrast, cycle sequencing reactions require a much higher level of primer quality independent of template type.

Mutations are commonly identified by PCR followed by direct sequencing of the PCR product. The primer quality is therefore even more critical when you want to detect or verify single base variants:

  • Genotyping by sequencing
  • Microsatellite analysis
  • Verification of SDM experiments

 


That means PCR primers should already have a very good quality when the same primers are used for sequencing the PCR product!


With our validated SeqPrimer, you can be sure to have the optimum primer quality to fulfil this requirement.

The SeqPrimer was developed in close cooperation with our experienced oligo synthesis and DNA sequencing team. It is specified based on a set of analytical quality criteria that were verified in thousands of sequencing reactions.


Standard primers and custom sequencing primers ordered along with DNA sequencing have the same stringent quality criteria.

 

Documentation

 

Documents are provided in your online account free of charge:

 

  • Oligo synthesis report
  • Quality report incl. MALDI-TOF MS spectra
  • Delivery note

 

 

 

 

Literature

 

 

 

 

 

 

 

 

Solutions you may also like

 

 

 

EVOCard


The prepaid card for more
flexibility in your research


>> READ MORE

 

 

 

PlateSeq Service


Sanger sequencing of all sample types in 96well plates


>> READ MORE

 

 

 

SeqPrimer NXP


The perfect sequencing primer next day at your bench


>> READ MORE

 

 

 

TubeSeq Service


Sanger sequencing in tubes with the extra bit


>> READ MORE

 

 

Quality is important for us

Our products and services are produced and performed under strict quality management and quality assurance systems.

Find certificates here