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    • DNA / RNA Oligos
      • Products & Services
      • DNA / RNA Oligos
        • qPCR application oligos
        • PCR Primers
        • qPCR Probes
        • Ultimate Precision Probes
        • Next Generation Sequencing Oligos
        • NGSgrade Oligos
        • NGSgrade Oligos 2.0
        • NGS UDI Primer Sets
        • Sanger Sequencing Oligos
        • SeqPrimer
        • Standard Primers
        • Cloning applications & CRISPR
        • Cloning Oligo
        • EXTEmers
        • Custom DNA & RNA Oligos
        • Custom DNA Oligos
        • Salt Free Oligos
        • Custom RNA Oligos
        • siMAX siRNA
        • Large Scale Oligos
        • Special Requests
        • Oligo Design Tools
        • Oligo Analysis Tool
        • PCR Primer Design Tool
        • qPCR Assay Design Tool
        • Sequencing Primer Design Tool
        • Helpful Links
        • Oligo Decision Tree
        • Oligo Excel Upload Forms

        • Prepaid Oligo Coupons
          A Unique Prepayment Option for PCR - and Sequencing Primers
          Learn more

           

           

    • Gene Synthesis & Molecular Biology
      • Products & Services
      • Gene Synthesis & Molecular Biology
        • Synthetic genes
        • Standard Genes
        • Express Genes
        • Complex Genes
        • Gene synthesis project
        • Gene Fragments
        • GeneStrands
        • Express GeneStrands
        • Molecular Biology Services
        • Plasmid Preparation
        • Site Directed Mutagenesis
        • DNA Cloning
        • Optimisation tool
        • GENEius
    • Sanger Sequencing
      • Products & Services
      • Sanger Sequencing
        • Sequencing Services
        • Mix2Seq Services
        • LightRun Services
        • TubeSeq Supreme
        • PlateSeq Supreme
        • Direct Colony Sequencing
        • Prepaid Products
        • Mix2Seq Kits
        • LightRun Barcodes
        • Barcode Labels & Coupons
        • PlateSeq Kits
        • Shipping Options
        • Sequencing Accessories
        • Sample Shipment
        • Additional Services
        • Sequencing Primers
        • Free Barcode Labels
        • Special Sequencing Services
        • Sequence Verification and Primer Walking under ISO 17025 & GLP
        • �
          Mix2Seq Kit
          Fastest Sanger sequencing of premixed samples in tubes.

          Order Now

           

           

    • Nanopore Sequencing
      • Products & Services
      • Nanopore Sequencing
        • ONT Lite Portfolio
        • Whole Plasmid Sequencing
        • Linear / Clonal Amplicons
        • Bacterial Genome Sequencing
        • Hybrid Assembly
        • Yeast Genome Sequencing
        • Prepaid ONT Coupons
        • Free barcodes
        • Genome Sequencing
        • Human Whole Genome Sequencing
        • Bacteria Whole Genome Sequencing
        • Whole Genome Sequencing Non-Human
        • Amplicon Sequencing
        • Custom Long-Read Amplicon Sequencing
        • 16S Full-Length Microbiome
        • Prepaid ONT Lite Coupons
          A Unique Prepayment Option for your sequencing with Oxford Nanopore

          Learn more

           

           

    • Next Generation Sequencing
      • Products & Services
      • Next Generation Sequencing
        • Genome Sequencing
        • Human WGS
        • Non-human WGS
        • INVIEW Resequencing
        • Microbiome & Metagenome
        • INVIEW Microbiome
        • INVIEW Metagenome
        • Amplicon Sequencing
        • Amplicon sequencing
        • Amplicon sequencing - incl. amplification
        • Transcriptome
        • INVIEW Transcriptome
        • INVIEW Transcriptome Bacteria
        • NGSelect RNA
        • Exome & Enrichment
        • INVIEW Exome
        • Clinical Research Exome
        • INVIEW Oncoprofiling
        • INVIEW Liquid Biopsy Oncoprofiling
        • Bioinformatic Services
        • RNA-Seq Analysis
        • Variant Analysis Service
        • Metagenome Analysis Service
        • Microbiome Profiling Analysis
        • Prepaid Coupons and free Barcodes
        • Free Barcodes
        • NGS Prepaid Coupons
        • Important information
        • Sample Submission Guidelines
        • NGS Publications
        • Special applications
        • INVIEW CRISPR Check
        • INVIEW Virus
        • Ready-to-Load
        • Prepaid NGS Coupons

           

          A Unique Prepayment Option for our NGS Services

          Learn more

           

           

    • Genotyping & Genexpression
      • Products & Services
      • Genotyping & Genexpression
        • Applied Genomic Services
        • DNA Barcoding
        • Cell Line Authentication
        • Mycoplasmacheck
        • Fragment Length Analysis
        • Genotyping Services
        • SNP Genotyping
        • Copy Number Variation
        • Mikrosatellites/ STR/FLA/ IDAA
        • Gene Expression Services
        • Transcriptome analysis
        • Expression Arrays
        • Target Gene Expression
  • Markets
    • Pharma / Biotech
      • Markets
      • Pharma / Biotech
        • Synthesis Products
        • Industrial-grade NGS Oligos
        • Ultimate Precision Probes
        • Special Oligo Requests
        • Large Scale Oligos
        • Gene Synthesis
        • Gene Fragments
        • Sequencing Services
        • Exome Sequencing
        • Transcriptome Sequencing
        • Genome Sequencing
        • Bioinformatic Solutions for NGS
        • Favorite Content
        • NGS - Disease Diagnostics
        • Cancer Ecology
        • Personalised Cancer Therapy
        • Revolutionising human-like-protein production
        • The Microbiome Of Cancer
        • All about biomarker discovery
    • Agrigenomics
      • Markets
      • Agrigenomics
        • Plant Breeder
        • DNA marker discovery
        • Marker-assisted selection
        • GRAS-Di®
        • Microbiome and metagenomics
        • Animal Breeder
        • DNA Marker discovery
        • Pathogen screening
        • Parentage testing
        • Genomic selection
        • Marker-assisted selection
        • Webshop - GenFarmEval
        • Favorite Content
        • Microarrays Accelerate Blue Biotechnology
        • How To Do NGS 50% Faster
        • NGS Portfolio
        • GenFarmEval.com

          Visit our Webshop for Farmers

          learn more

    • Consumer Genomics
      • Markets
      • Consumer Genomics
        • Sequencing Services
        • Genotyping
        • Epigenome Profiling
        • Microbiome Analysis
        • Shotgun Sequencing
        • Whole Genome Sequencing
        • Additional Services
        • Sample Collection Kits
        • Shipping
        • DNA Extraction
        • Laboratory Service
        • Biobanking
        • Favorite Content
        • Population Genetics
        • The End of Gene Doping
        • Home Genomics Testing
        • IVDR Compliance
    • Food & Environment
      • Markets
      • Food & Environment
        • Food Testing
        • Food Authenticity
        • Meat Traceability
        • Pathogen Traceability
        • Cannabis and Hemp Testing
        • Environmental Testing
        • Non-targeted detection of organisms / species
        • Targeted detection of organisms / species
        • eDNA Tracker
        • Favorite Content
        • Determine the Source of Meat
        • Pine Nuts – Why Testing For Edibility Matters
    • Diagnostic Kit Producer
      • Markets
      • Diagnostic Kit Producer
        • Synthesis Products
        • Large Scale Oligos
        • Special Requests in Tubes
        • Quality Assurance
        • GLP
        • ISO 17025
        • ISO 13485
        • Favorite Content
        • Oligonucleotides For Diagnostics
        • The Future of RNA Applications
    • Research / Biotech
      • Markets
      • Research / Biotech
        • Favorite Services
        • Custom DNA Oligos
        • TubeSeq Service Sanger
        • INVIEW Microbiome NGS
        • NGS Services
        • GeneStrands
        • Express Services
        • Mix2Seq NightXpress
        • Express Genes
        • Express GeneStrands
        • Favorite Content
        • Eurofins Genomics Goes Green
        • GENEius – Codon Usage Optimisation
        • 5 tips to speed up your qPCR
        • Why salt-free oligos are the unsung hero of molecular biology
  • Resources
    • Account
      • Resources
      • Account
        • My profile
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Cloning Service

   Intro & Info

Eurofins Genomics’ cloning services includes TA cloning, cloning of full length cDNA or PCR products, synthetic genes or subcloning of vector inserts.

By default we will classify your project as "standard cloning" service. After submitting your order or quote our team will check your project and if you submitted an order for a complex cloning service (which leads to higher costs) we will contact you immediately.


Settings for our standard cloning service are:
  • Subcloning of a standard insert (200 – 6,000 bp) or PCR product into a high-copy standard E. coli compatible vector (max. 7000 bp)
  • Subcloning via two different sticky end restriction enzymes
  • Biosafety level 1

Please be aware that the following options are not available for mini and midi prep
  • Endotoxin-free preparation
  • Sterile preparation
  • Animal-free preparation
  • Bioburden test
  • LAL test

Service description
  • Quality control of vector(s)
  • Development of the cloning strategy and subcloning
  • Cloning Verification: We analyse up to 6 clones via restriction digest and/or sequencing of the restriction sites (2 runs)
  • Documentation: By quality assurance report, including project specifications, complete plasmid description, restriction map and DNA sequence information
  • Please note that there can be no guarantee for molecular biology projects
  • In case the subcloning fails even after one repetition we would have to charge a set up fee of 30% per subcloning

Confidentiality

It stands to reason that Eurofins Genomics will perform the services outlined above on a “fee for service” basis. This means that Eurofins Genomics has and will claim no rights whatsoever to all or any of the data that will be generated. Eurofins Genomics will take all necessary measures to ensure that none of the data will be disseminated to third parties.

Risk group classification sheet

Please download the risk group classification sheet and send the filled form to genes-eu[at]genomics.eurofinseu.com after placing your order.

Cloning Service Order Form

Synthetic gene order information open / close
Vector name
Antibiotic Resistance
Please provide this antibiotic to Eurofins Genomics
Vector Size [bp]
bp
Copy Number Information
Complete vector sequence
The sequence is valid
Gene sequence had illegal characters and we cleaned it up for you
Set as default
open / close
Scale
Biosafety Level
Please contact our support team at support-eu@genomics.eurofinseu.com or via phone +49 7531 81 60 68 (toll-free phone numbers)
Endotoxin-Free Prep
Sterile
Animal-free Prep
Bioburden Testing
Endotoxin Testing (LAL)
Set as default
Insert Information open / close
Insert name
DNA sequence of the insert
The sequence is valid
Gene sequence had illegal characters and we cleaned it up for you
Upload of data
Upload correct file
xls, xlsx, csv, pdf, txt, doc are the allowed formats
Donor Vector details
Size of the final contruct
Restriction sites (5')
Restriction sites (3')
Set as default
Your Project open / close
Remarks:
Set as default
 
 
Contact Us

TECHNICAL SUPPORT

Phone: +49 7531 816068

E-Mail: support-eu@genomics.eurofinseu.com

HOURS

Mon-Thu: 8 : 00 AM – 6 : 00 PM, ET

Fri: 8 : 00 AM – 6 : 00 PM, ET

TOLL FREE PHONE NUMBER

Direct Line: 00800-200 100 20

E-Mail: support-eu@genomics.eurofinseu.com

HOURS

Mon-Fri: 8 : 00 AM – 3 : 00 PM, ET

QUOTES, PRICING & SPECIAL REQUESTS

Quotes are submitted, reviewed, and accepted through the online quoting tool. Learn more.
Please direct inquires about pricing and special project requests to your sales representative.
General questions: support-eu@genomics.eurofinseu.com

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