For your convenience, please use our Oligo Analysis Tool or apply the formula below:
N [nmol] = OD x 100 / 1.54 x nA + 0.75 x nC + 1.17 x nG + 0.92 x nT
n = number of nucleosides of the type X
Example:
Sequence: GAA ATG AGT GCT CAT CAC TAC TTC CGC (27mer)
nA=7; nC=8; nG=5; nT=7
OD=12 (measured)
N [nmol] = 12 x 100 / 1.54 x 7 + 0.75 x 8 + 1.17 x 5 + 0.92 x 7
= 41.3