DNA & RNA Oligonucleotides FAQs

How do I anneal complementary oligos?

Dissolve the lyophilised oligonucleotide in water or TE. Use the concentrations of your oligonucleotide solutions stated on the oligonucleotide synthesis report.
Add the following components together:

Stock                                             Final Concentration
Oligonucleotide 1                             100 nmole/ml
Oligonucleotide 2                             100 nmole/ml
10x annealing buffer*                      1x
Nuclease-free water                          to appropriate volume

*10x annealing buffer: 100 mM Tris-HCI, pH 7.5,1 M NaCI and 10 mM EDTA.
Heat the oligo solution to a temperature 10°C higher than the calculated melting temperature. Maintain the temperature for 10 minutes. Remove the solution from the heating block/water bath and allow it to cool slowly to room temperature on the bench (approximately 1 hour).
Store the annealed oligos at -20°C as recommended.