The right answers to frequently asked questions
Find the answers to all our products and services by clicking the links below.
Which oligo product fits to my application?
As a matter of course the quality of our oligonucleotides should consistently fulfil the performance criteria of your application and arrive in your lab right on time.
For the most common applications like PCR, qPCR, DNA cloning and DNA sequencing by Sanger we offer pre-defined and optimised primer and probes validated in respective applications.
Please see Optimised Application Oligos overview.
For all applications which are not covered by our Optimised Application Oligos we offer customised DNA & RNA oligos where you can specify your oligo according to your individual requirements.
Please see Customised DNA & RNA Oligo overview.
What does HPSF mean?
HPSF stands for High Purity Salt Free. It refers to a purification method using gel filtration plates in a 96-well format, combined with centrifugation.
This size exclusion technique removes small molecular weight impurities, including salts, protecting groups, and short n–x sequences (typically <10mers).
lease note that longer n–x sequences, such as a 25mer in a 35mer synthesis, may not be fully separated using this method. HPSF purification is available for oligonucleotides up to 120 bases in length.
Which quality controls do you perform?
OD measurement
The final yield of an oligo is determined by measuring the OD (Optical Density) values. One OD260 unit of DNA is the amount of DNA that gives an absorbance reading of 1.0 at a wavelength of 260 nm, for a sample dissolved in 1.0 ml total volume of ddH20 which is read in a 1 cm quartz cuvette. 1 OD260 corresponds to approx. 33 µg/ml of single stranded DNA, depending on the GC content.
MALDI-TOF MS
To ensure the identity and qualitative purity of each and every synthesised oligonucleotide, we use the latest generation of Sequenom MALDI-TOF (Matrix Assisted Laser Desorption Ionisation - Time of Flight) mass spectrometers. The high grade of automation and the use of proprietary software to analyse the spectra ensures that we deliver oligonucleotides of highest quality.
What are EXTREmers?
EXTREmers are suitable for use in several molecular biology and synthetic biology applications that require long oligos with high sequence accuracy.
EXTREmers allow us to provide you with
- Highest coupling efficiency of ≥ 99.5%
- Lowest error rates of typically 0.09 %
- Best cloning results with > 85 % perfect clones
Specifications:
- Sequence length from 60 - 200 bases
- Normalised to 4 nmol dried down
- Wobbles (non-defined ratio) possible
- Production in 5-8 working days
- QC by ESI-MS