The right answers to frequently asked questions
Find the answers to all our products and services by clicking the links below.
How do you optimise synthetic genes?
Using our proprietary software GENEius, the supplied amino acid or DNA sequences will be optimised and adapted e.g. to the codon usage of any organism. Also repeats or hairpin structures are avoided and the GC content is adjusted.
GENEius is also available as a free online tool for our customers.
What lengths of synthetic DNA can you synthesise?
We can synthesise sequences or complete genes from very short fragments up to several thousand base pairs in length. Please contact us for a quote.
What is the difference between a long oligo and a synthetic gene?
Oligos are, due to chemical reasons, limited in length (max. 120-200 bases) and single stranded. The length of a synthetic gene might extend to several thousand base pairs. Synthetic genes are double stranded and generally cloned.
What is the difference between genes delivered cloned into a vector and delivered as linear PCR product?
Although the sequence of the linear gene will have been analysed and sequence congruence will be 100%, the PCR product represents a mixture of correct and incorrect sequences. Therefore, after subcloning not all clones will show the expected sequence. We recommend analysing 6-12 clones for genes of 300-950 bp.
For cloned genes you receive plasmids with the 100% correct sequence.
Do you offer cloning into a vector of my choice?
Yes. We offer additional subcloning of your gene into any vector, for example expression vectors.
Please send your specific vector1 for subcloning together with the printout of the order confirmation to the following address:
Eurofins Genomics
Gene Synthesis Laboratory
Anzinger Str. 7a
85560 Ebersberg
Germany
Which scales of plasmid preparation do you offer?
We offer the following preparation scales:
- Mini scale plasmid preparation: 1-10 µg Plasmid-DNA
- Midi scale plasmid preparation: 15-50 µg Plasmid-DNA
- Maxi scale plasmid preparation: 100-500 µg Plasmid-DNA
- Mega scale plasmid preparation: 500 µg-2.5 mg Plasmid-DNA
- Giga scale plasmid preparation: up to 10 mg Plasmid-DNA
More information about our plasmid preparation service can be found here
What quality standards can I expect?
We verify each synthetic gene via double-stranded DNA sequencing by our in-house sequencing service. We ensure 100% sequence accuracy of every cloned gene.
The high quality standard of the complete synthesis process is permanently assured by our professional quality management.
Gene and GeneStrands Turnaround times (TAT) (including TAT for SARS-CoV-2 genes/GeneStrands)
Turnaround times for Genes
The standard turnaround times (plus one day for the shipment) are:
| Standard Genes (up to 1000 bp) |
6-8 working days |
| Standard Genes < 2000 bp |
15 - 20 working days |
| Standard Genes > 2000 bp |
5 additional working days / kbp |
| Subcloning |
additional 5 - 10 working days |
| Complex Genes |
additional 5 - 10 working days |
The turnaround times for our Express Genes are:
| Express Genes (up to 1000 bp) |
4 working days |
| Express Genes (up to 1500 bp) |
6 working days |
| Express Genes (up to 2000 bp) |
7 working days |
| Express Genes (up to 3000 bp) |
11 working days |
| Express Genes (up to 4000 bp) |
13 working days |
| Express Subcloning |
additional 4 working days |
Turnaround time for GeneStrands
The synthetic linear dsDNA will be delivered as a fragment.
| Gene Strands up to 1 kbp |
5 -7 working days |
| Gene Strands up to 2 kbp |
8 - 10 working days |
| Express GeneStrands up to 1 kbp |
1 -2 working days |
Turnaround times for SARS-CoV-2 Genes
| Standard Genes (up to 1 kbp) |
10 - 15 working days |
| Standard Genes < 2 kbp |
15 - 20 working days |
| Standard Genes 2 - 3 kbp |
20 - 25 working days |
| Standard Genes 3 - 4 kbp |
25 - 30 working days |
| Complex Genes |
additional 5 - 10 working days |
Turnaround times for SARS-CoV-2 GeneStrands
| GeneStrands up to 1 kbp |
6 - 8 working days |
| GeneStrands 1 - 2 kbp |
9 - 11 working days |
Will my data be treated confidentially?
From the synthesis of the oligos, over the gene synthesis itself up to the quality control by DNA sequencing, Eurofins Genomics offers a complete in-house process, to ensure 100% confidentiality.