FAQs Gene Synthesis

Using our proprietary software GENEius, the supplied amino acid or DNA sequences will be optimised and adapted e.g. to the codon usage of any organism. Also repeats or hairpin structures are avoided and the GC content is adjusted.
GENEius is also available as a free online tool for our customers.

We can synthesise sequences or complete genes from very short fragments up to several thousand base pairs in length. Please contact us for a quote.

Oligos are, due to chemical reasons, limited in length (max. 120-200 bases) and single stranded. The length of a synthetic gene might extend to several thousand base pairs. Synthetic genes are double stranded and generally cloned.

Although the sequence of the linear gene will have been analysed and sequence congruence will be 100%, the PCR product represents a mixture of correct and incorrect sequences. Therefore, after subcloning not all clones will show the expected sequence. We recommend analysing 6-12 clones for genes of 300-950 bp.
For cloned genes you receive plasmids with the 100% correct sequence.

Yes. We offer additional subcloning of your gene into any vector, for example expression vectors.

Please send your specific vector¹ for subcloning together with the printout of the order confirmation to the following address:

Eurofins Genomics
Gene Synthesis Laboratory
Anzinger Str. 7a
85560 Ebersberg
Germany

¹ A minimum of 10 µg vector DNA is required - either lyophillised or in solution (c=200 ng/µl).

We offer the following preparation scales:

• Mini scale plasmid preparation: 1-10 µg Plasmid-DNA
• Midi scale plasmid preparation: 15-50 µg Plasmid-DNA
• Maxi scale plasmid preparation: 100-500 µg Plasmid-DNA
• Mega scale plasmid preparation: 500 µg-2.5 mg Plasmid-DNA
• Giga scale plasmid preparation: up to 10 mg Plasmid-DNA

More information about our plasmid preparation service can be found here

We verify each synthetic gene via double-stranded DNA sequencing by our in-house sequencing service. We ensure 100% sequence accuracy of every cloned gene.

The high quality standard of the complete synthesis process is permanently assured by our professional quality management.

Turnaround times for Genes

The standard turnaround times (plus one day for the shipment) are:

The turnaround times for our Express Genes are:

Turnaround time for GeneStrands

The synthetic linear dsDNA will be delivered as a fragment.

Turnaround times for SARS-CoV-2 Genes

Turnaround times for SARS-CoV-2 GeneStrands

From the synthesis of the oligos, over the gene synthesis itself up to the quality control by DNA sequencing, Eurofins Genomics offers a complete in-house process, to ensure 100% confidentiality.