You will be informed if your sample material does not meet our IQC criteria and will be provided with information about available options. In certain cases, processing at your own risk may be offered. In this case, guarantees for the successful creation of the library and sequencing will be void. If the library fails the quality control prior to sequencing, our Customer Care team will also contact you.
When processing sample material beyond the specs, several issues may arise and should be carefully considered:
- Decreased RNA Quality: Sample material that deviates from optimal conditions (e.g., improper storage, extended handling, or delays) may lead to RNA degradation, resulting in poor quality data.
- Lower Input Quantity: Reduced input RNA quantity can lead to insufficient material for accurate analysis, potentially causing biased or incomplete results due to low coverage or poor detection of lowly expressed genes. These samples may also fail to produce sufficient amounts of libraries for subsequent sequencing.
- Bias in Data: Alterations in sample handling or processing can introduce biases in gene expression profiles, such as uneven gene-body coverage, particularly in the 3' or 5' ends.
- Reduced Sensitivity: Handling samples outside of recommended conditions can affect the detection of lowly expressed genes, making the analysis less sensitive.