Site Directed Mutagenesis - For Any Downstream Application

Site-directed mutagenesis solutions

Site-directed mutagenesis - Precise DNA modifications

Site-directed mutagenesis can help reveal the secrets of gene regulation

Site-directed mutagenesis (SDM) enables precise and targeted DNA modifications empowering you to investigate gene regulation, protein function, and structure-function relationships with exceptional accuracy. For projects requiring several or more complex mutations, our Gene Synthesis service is often the faster and more flexible solution. It allows you to introduce multiple sequence changes at once offering maximum flexibility in your design.

If you would like to receive a quote for a SDM project, please provide us with the original sequence along with the desired mutation site(s).

If available, please also send us your final construct.

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Our site-directed mutagenesis service includes:

Project Intake & Assessment
We review all relevant project details — your sequence, mutation sites or regions, vector information, and final construct — to define the optimal approach for your mutagenesis workflow.
Primer & Strategy Design
Using advanced design software, we create high specificity primers and a reliable SDM strategy. This ensures optimal binding efficiency while avoiding dimers and secondary structure interference.
Mutagenesis Reaction & Construct Preparation
Based on your mutation type, we select the most appropriate mutagenesis kit and method to deliver fast, precise, and reproducible results.
Specific Amplification Method
Our workflows use highly specific amplification methods to minimize errors and ensure exceptional sequence quality.

100% Verified Accuracy
Every mutated region undergoes Sanger sequencing, with optional restriction digest analysis available at an additional cost, to ensure complete accuracy and integrity.
Delivery & Documentation
You receive lyophilized plasmid DNA together with a complete quality report, sequencing chromatograms, and all sequence files. Glycerol stocks are available on request.
Guaranteed Confidentiality
All sensitive steps are performed exclusively at our DNA campus: DNA oligonucleotide synthesis, Sanger sequencing, and the SDM process. Your data never leaves our facility.
Long-Term SDM Expertise
Our experienced laboratory team has been performing SDM for many years, continuously refining techniques and ensuring consistent, high quality results.

Possible SDM approaches

Point mutations - a small number of base exchanges at one target site within a gene
Insertions - from a few bases up to long strechtes of additional bases
Deletions - remove unwanted parts of your gene sequence
Large substitutions - a longer stretch with several mutations at one target site
Multi-position exchanges - alterations of the gene throughout many positions or the complete gene sequence