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    Cell Line Authentication

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    • Genotyping & Gene Expression >
    • Applied Genomics Services >
    • Cell Line Authentication
     

    Human Cell Line Authentication


    Achieve an easy workflow with your cell line.

     

    Order now

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    >> Send request

     

    Dear customer, if you send your samples via our dropboxes, please make sure to order the orange stickers below directly with your barcodes. This will help to reduce our carbon footprint. Thank you very much.

     

     


    Prepaid CLA Barcode

    Cell Line Authentication Barcodes


    Convenient sample tracking.

    • Order your barcodes in advance
    • Label your samples and benefit from secure sample assignment
     
     
     
    Quantity:

     
     
    Price:
    109,00 €
     
         

     

     

    Cell line authentication barcodes remain valid for five years from the date of ordering, after which they expire.



    Want to use our Dropboxes to send your samples?

    Please order the stickers below and put them on the bag / box where your samples are shipped in  (within the envelope). This will ensure that your samples are routed savely and fast to our cell line testing laboratory.

    Please find your closest dropbox location here >>

    More information about our dropbox system can be found here>>

     


    Stickers for your Tube Bags

    Sticker to send your CLA samples via our Dropboxes


    Convenient sample shipment.

    • One unit includes 10 shipment stickers
    • You only need one sticker per shipment
     
     
     
    Quantity:

     
     
    Price:
    0,00 €
     
         

     

     

     

     

     

    Why cell line testing?

     

    You want to publish your results with certified cell lines in peer reviewed journals (e.g. Nature, AACR Journals, Cell Biochemistry and Biophysics, etc.)? Eurofins will help you with the mandatory cell line authentication:

    • Save time with our fast and reliable service for cell line authentication.
    • Don't risk working with misidentified or cross-contaminated cell lines.
    • Check for unwanted microorganisms (e.g. Mycoplasma)

     

    Your results in just 5 working days

     

    We will upload your DNA (STR) profile results as a PDF file into your MyOrder section in our webshop within 5 working days. All our certificates are accepted by peer-reviewed journals.

     

     

    Note: for Cell Line Authentication testing (CLA) you need to order barcodes first

     

     
     

     

     

     Product details, sample submission & publications

     

     

    Sample Submission

    How to order & send samples for Eurofins' Cell Line Authentication Services

     

    Barcode Ordering & Sample Submission

    Order up to 100 CLA Basic Service Prepaid Barcodes in advance and benefit from our best price per sample - independent from sample batch size per single order.

     

    How to order CLA with prepaid barcodes:

    1. Click on the 'Order Now' Button on the top.
    2. Follow the steps to register your samples and barcodes.
    3. Send your samples directly to below address or place them into one of your dropboxes (+approx. one day logistics time)

     

    General Shipment Address:

     

    APG Department
    Eurofins Genomics
    Anzinger Str. 7a
    85560 Ebersberg
    Germany

     

    Extracted DNA or cell pellet? Your choice without additional cost. Send your samples as:

     

    Genomic DNA
    • 2 mL reaction tube; volume >20 µL; amount >10 ng/µl
    • Shipped at ambient temperature
    • If >20 samples per batch are shipped, please sort the 2 mL tubes in a box

     

    Cell pellet
    • 2 mL reaction tube (no falcon tubes) with >10.000 cells (visible cell pellet!)
    • Cell culture medium completely removed
    • Cell pellets washed with TE buffer or PBS
    • Shipped at ambient temperature or cooled (no need for dry ice)
    • If >20 samples per batch are shipped, please sort the 2 mL tubes in a box.

     

    Service portfolio

    Basic human cell line authentication service

    • Cell Line Authenticity Basic Service achieves genotyping according to ANSI/ATCC standard ASN-0002. For this service, please send your cell pellets or DNA extracts to test 16 DNA markers using the Applied BiosystemsTM AmpFLSTRTM IdentifilerTM Plus PCR Amplification Kit system.

     Additional cell line authentication services

    • DNA extraction of standard cell line samples like cell pellets (dried or frozen)
    • Profile search and comparison to published profiles using public and recognized databases (DSMZ, Cello)
    • Provision of certificates for proof of testing results (e.g. for publication)
    • Comparison to patient reference samples

       As part of a custom project we also offer:

    • DNA extraction of non-standard cell line samples like biopsy samples, blood, tissue, etc.
    • Species identification to determine contamination of cell lines by other species (pig, human, hamster, bovine, ovine, mouse, etc.)
    • Genome Instability Monitoring and CNV testing

     

    For additional services please send us a request >>

     

    Publications

    Benefit from best practice

     

    With more than 20 years of experience in preparing human and animal DNA/STR profiles, for many years now Eurofins works closely with national and international police forces and public authorities. You can rely on our results. Please don't hesitate to contact us and to trust us with your cell lines.

     

    We recommend cell line authentication at regular intervals as a standard quality assurance to ultimately save time and money.

     

    Find here a list of publications, where customers were using our CLA services to confirm the authenticity of their cell lines.

     

    2022: 

    Cell Line Authentication: Heppt et. al., HDAC2 Is Involved in the Regulation of BRN3A in Melanocytes and Melanoma

    Cell Line Authentication: Sabat-Pośpiech et. al., Aggressive uveal melanoma displays a high degree of centrosome amplification, opening the door to therapeutic intervention

     

    2021

    Cell Line Authentication: Nguyen et. al., Impact of DNA repair and reactive oxygen species levels on radioresistance in pancreatic cancer

    Cell Line Authentication: Inzunza et. al., Generation of nonviral integration-free human iPS cell line KISCOi001-A from normal human fibroblasts, under defined xeno-free and feeder-free conditions

    Cell Line Authentication: Graffmann et. al., Generation of a Crigler-Najjar Syndrome Type I patient-derived induced pluripotent stem cell line CNS705 (HHUUKDi005-A)

    Cell Line Authentication: Boehnke et. al., CRISPR/Cas9 mediated CXCL4 knockout in human iPS cells of polycythemia vera patient with JAK2 V617F mutation

    Cell Line Authentication: Koch et. al., Three dimensional cultivation increases chemo- and radioresistance of colorectal cancer cell lines

    Cell Line Authentication: Grote et. al., Adapter Chimeric Antigen Receptor (AdCAR)-Engineered NK-92 Cells for the Multiplex Targeting of Bone Metastases

    Cell Line Authentication: Baath et. al., MET Expression and Cancer Stem Cell Networks Impact Outcome in High-Grade Serous Ovarian Cancer

    Cell Line Authentication: Mygland et. al., Identification of response signatures for tankyrase inhibitor treatment in tumor cell lines

    Cell Line Authentication: Kirstein et. al., Lomeguatrib Increases the Radiosensitivity of MGMT Unmethylated Human Glioblastoma Multiforme Cell Lines

    Cell Line Authentication: Austermeier et. al., Albumin Neutralizes Hydrophobic Toxins and Modulates Candida albicans Pathogenicity

    Cell Line Authentication: Balgoma et. al., Anthracyclins Increase PUFAs: Potential Implications in ER Stress and Cell Death

     

    2020

    Cell Line Authentication: Natoli et. al., Human ovarian cancer intrinsic mechanisms regulate lymphocyte activation in response to immune checkpoint blockade

    Cell Line Authentication: Munir et. al., Abundance, fatty acid composition and saturation index of neutral lipids are significantly different between isogenic primary and metastatic colon cancer cell lines

    Cell Line Authentication: Stroomberg et. al., Novel functions of the luteinizing hormone/chorionic gonadotropin receptor in prostate cancer cells and patients

    Cell Line Authentication: Zaheed et. al., A bioinformatics approach to identify novel long, non-coding RNAs in breast cancer cell lines from an existing RNA-sequencing dataset

    Cell Line Authentication: Crawford et. al., The E3 ligase HUWE1 inhibition as a therapeutic strategy to target MYC in multiple myeloma

     

    2019

    Cell Line Authentication: Navarro et. al., Generation of induced pluripotent stem cell lines from two Neuroblastoma patients carrying a germline ALK R1275Q mutation

    Cell Line Authentication: Giatromanolaki et. al., CYP17A1 and Androgen-Receptor Expression in Prostate Carcinoma Tissues and Cancer Cell Lines

    Cell Line Authentication: Yudhistiara et. al., Carbon ion and proton beam irradiation of a normal human TK6 lymphoblastoid cell line within a magnetic field of 1.0 tesla

    Cell Line Authentication: Yudhistiara et. al., The influence of a magnetic field on photon beam radiotherapy in a normal human TK6 lymphoblastoid cell line

    Cell Line Authentication: Mattern et. al., ADAM15 mediates upregulation of Claudin-1 expression in breast cancer cells

    Cell Line Authentication: Ehrenhöfer-Wölfer et. al., SMARCA2-deficiency confers sensitivity to targeted inhibition of SMARCA4 in esophageal squamous cell carcinoma cell lines 

    Cell Line Authentication: Hood et. Ral., Isoform-specific Ras signaling is growth factor dependent 

    Cell Line Authentication: Song et. al., Transcriptomic analysis reveals cell apoptotic signature modified by heparanase in melanoma cells

     

    2018

    Cell Line Authentication: Rogers et. al., Limitations of current in vitro models for testing the clinical potential of epigenetic inhibitors for treatment of pediatric ependymoma

    2017

    Cell Line Authentication: Uhlin et. al., Derivation of human iPS cell lines from monozygotic twins in defined and xeno free conditions

    Cell Line Authentication: Østevold et. al., Septin remodeling is essential for the formation of cell membrane protrusions (microtentacles) in detached tumor cells

     

    2016

    Cell Line Authentication: Hantel et. al., Targeting heterogeneity of adrenocortical carcinoma: Evaluation and extension of preclinical tumor models to improve clinical translation

     

     

     

     

     Literature

     

    Sample Submission Guide

    ICLAC: International Cell Line Authentication Comittee 

    ICLAC: Register of misidentified cell lines

     

     

     

     

     

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