NGS Applications

Olink Explore - 3072 targets using NGS

Protein Biomarker Detection using the Olink^® Antibody Technology.

Welcome to Eurofins Genomics, your trusted partner in genomic services. We are thrilled to introduce our latest product offering: Protein expression measurement using NGS and Olink^® 3072 Explore.

>> contact us

>> request a quote

Application

Protein expression analysis is a crucial component in the study of gene function, cellular signaling, and disease mechanisms.

Traditional methods for measuring protein expression, such as western blotting and ELISA, are often limited in their scope and throughput.

That's where our new product comes in - using Next-Generation Sequencing (NGS) and Olink^® 3072 Explore technology, we can now measure protein expression on a larger scale and with higher accuracy.

How does it work?

>> assay list

Product Specifications & Ordering

Starting material

Starting material: Plasma or Serum

Volume: at least 50 µl

Concentration: 0.5 - 1 µg/µl total protein conc.

Available Olink®; Panels for Olink®; Explore 3072

There are 8 different panels that sum up to the 3072 proteins to be analysed.

You can either order all 8 panels, 4 panels, 2 or 1 panel.

Sequencing workflow

Protein Enrichment with OLINK® Explore Panels
Illumina sequencing
- Run type: Paired end
- Read length: 2 x 50 bp
- 1 channel per panel
Deliverables: CSV file (NPX value), quality report (pdf)

Minimum number of samples: 88

Deliverables

Data in Normalized Protein Expression (NPX) format for relative quantification
Certificate of Analysis showing quality details – internal and external controls are included throughout the workflow to ensure data quality

Delivery time

Depending on the number of samples and number of panels.

Olink^® Target

In addition to Olink^® Explore 3072, we are pleased to provide access to the comprehensive Olink^® Target portfolio and Olink^® Flex. We pride ourselves on being your preferred partner, ready to assist with any requirements you may have. Please don't hesitate to reach out to us, and we will be more than happy to assist you with your needs.