Optimized sensitivity and specificity with our (q)PCR Primers
Developed, tested and verified by our real-time PCR specialists.
With our PCR Primers, you can be assured to receive the best suitable primer for every kind of standard and real-time PCR assay:
- Quantitative real-time PCR (qPCR) with SYBR Green / EvaGreen
- Probe based qPCR (single- and multiplex assays)
- Single- and multiplex end-point PCR
- Reverse-transcriptase PCR (rt-PCR)
- Nested PCR & other standard PCR protocols
The optimised and validated primers are available as
Read more what you can expect from our PCR Primer.
The optimal production parameters are predefined on the order pages. All you need to do is enter the name and sequence.
We recommend our SeqPrimer when the same primers are used for PCR and subsequently for sequencing the PCR products.
12PP
What you can expect from our PCR Primer
High PCR efficiency & specificity:
- Accurate and reliable primer quantification
Variabilities of maximum +/- 10% are guaranteed
- Stable primer Tm due to constant low salt concentration
Salts and cleaved protecting groups are efficiently removed during the manufacturing process
- Reproducible and reliable high purity of > 80%
Verified by UPLC* and controlled by MALDI-TOF MS
Higher sensitivity in assays with low DNA concentrations:
All relevant documents are provided online free of charge:
- Oligo synthesis report
- Quality report incl. MALDI-TOF MS spectra
- Delivery note
Additional online features free of charge:
- Selectable label layouts for your oligo tubes
- Complementary sequence calculator with direct ordering
- Online tracking of order process and delivery
- Personal order history
- PCR Primer Design Tool
- Oligo Analysis Tool
With our ISO 13485 certified production process, the PCR Primer can be used in in-vitro diagnostic (IVD) products and applications.
Reliable & predefined product specifications
(q)PCR Primer in Tubes and Plates:
- Sequence length from 15 - 35 bases
- Average synthesis yields of 25 nmol (5 OD)
- PCR Primer in tubes are delivered lyophilized
- PCR Primer in plates are delivered liquid at selected concentration
- Production in tube format in 1 working day
- Production of up to 5 plates in 3-4 working days
LocNA Primers:
- Sequence length from 10 - 35 bases
- Up to 8 LocNA nts per primer are possible, but maximum 4 LocNA nts are recommended
- Only DNA bases are allowed at the 3' end
- Average synthesis yields of 4.5 OD resp. 20 nmol
- Delivered lyophilized or concentration adjusted in tubes
- Production takes 3 working days
(q)PCR Primer NightXpress
- Sequence length from 15 to 35 bases
- Guaranteed synthesis yield of 1 OD
- Delivered lyophilized in tubes the next working day